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Insight into Bacillus cereus Associated with Infant Foods in Beijing

This study was undertaken to investigate the prevalence, antimicrobial resistance, and virulence gene profiles of Bacillus cereus in different brands of infant formula in Beijing supermarkets. Eighty-eight Bacillus cereus isolates were recovered in sixty-eight infant formulas of five domestic brands...

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Autores principales: Wang, Ping, Zhao, Xiaomei, Qu, Tianming, Liang, Lijiao, Ji, Qinglong, Chen, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909854/
https://www.ncbi.nlm.nih.gov/pubmed/35267350
http://dx.doi.org/10.3390/foods11050719
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author Wang, Ping
Zhao, Xiaomei
Qu, Tianming
Liang, Lijiao
Ji, Qinglong
Chen, Ying
author_facet Wang, Ping
Zhao, Xiaomei
Qu, Tianming
Liang, Lijiao
Ji, Qinglong
Chen, Ying
author_sort Wang, Ping
collection PubMed
description This study was undertaken to investigate the prevalence, antimicrobial resistance, and virulence gene profiles of Bacillus cereus in different brands of infant formula in Beijing supermarkets. Eighty-eight Bacillus cereus isolates were recovered in sixty-eight infant formulas of five domestic brands and fourteen imported brands. The prevalence rate in domestic and imported samples were 70.6% and 52.9%, respectively. Lower mean prevalence level was found in domestic samples (1.17 MPN/g) compared with the imported samples (3.52 MPN/g). Twenty-four virulence gene profiles were found, and most strains carried at least one virulence gene. The prevalence of nheA, nheB, nheC, cytK, bceT, and entFM in domestic and imported brand samples was similar. The occurrence of enterotoxin genes hblA, hblC, and hblD in domestic samples were 22.2%, 27.8%, and 22.2%, respectively, which was significantly higher than imported samples. Antimicrobial drugs-susceptibility analysis showed that all isolates were susceptible to gentamincin, amikacin, and ciprofloxacin; 38%, 7%, and 2.3% were resistant to rifampin, tetracycline, and chloramphenicol, respectively; and only one isolate was resistant to trimethoprim-sulfamethoxazole. Moreover, the cell numbers of Bacillus cereus in prepared infant formula increased rapidly at room temperature. Thus, monitoring guidelines are needed for accepted levels of Bacillus cereus in infant formula.
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spelling pubmed-89098542022-03-11 Insight into Bacillus cereus Associated with Infant Foods in Beijing Wang, Ping Zhao, Xiaomei Qu, Tianming Liang, Lijiao Ji, Qinglong Chen, Ying Foods Article This study was undertaken to investigate the prevalence, antimicrobial resistance, and virulence gene profiles of Bacillus cereus in different brands of infant formula in Beijing supermarkets. Eighty-eight Bacillus cereus isolates were recovered in sixty-eight infant formulas of five domestic brands and fourteen imported brands. The prevalence rate in domestic and imported samples were 70.6% and 52.9%, respectively. Lower mean prevalence level was found in domestic samples (1.17 MPN/g) compared with the imported samples (3.52 MPN/g). Twenty-four virulence gene profiles were found, and most strains carried at least one virulence gene. The prevalence of nheA, nheB, nheC, cytK, bceT, and entFM in domestic and imported brand samples was similar. The occurrence of enterotoxin genes hblA, hblC, and hblD in domestic samples were 22.2%, 27.8%, and 22.2%, respectively, which was significantly higher than imported samples. Antimicrobial drugs-susceptibility analysis showed that all isolates were susceptible to gentamincin, amikacin, and ciprofloxacin; 38%, 7%, and 2.3% were resistant to rifampin, tetracycline, and chloramphenicol, respectively; and only one isolate was resistant to trimethoprim-sulfamethoxazole. Moreover, the cell numbers of Bacillus cereus in prepared infant formula increased rapidly at room temperature. Thus, monitoring guidelines are needed for accepted levels of Bacillus cereus in infant formula. MDPI 2022-02-28 /pmc/articles/PMC8909854/ /pubmed/35267350 http://dx.doi.org/10.3390/foods11050719 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Ping
Zhao, Xiaomei
Qu, Tianming
Liang, Lijiao
Ji, Qinglong
Chen, Ying
Insight into Bacillus cereus Associated with Infant Foods in Beijing
title Insight into Bacillus cereus Associated with Infant Foods in Beijing
title_full Insight into Bacillus cereus Associated with Infant Foods in Beijing
title_fullStr Insight into Bacillus cereus Associated with Infant Foods in Beijing
title_full_unstemmed Insight into Bacillus cereus Associated with Infant Foods in Beijing
title_short Insight into Bacillus cereus Associated with Infant Foods in Beijing
title_sort insight into bacillus cereus associated with infant foods in beijing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909854/
https://www.ncbi.nlm.nih.gov/pubmed/35267350
http://dx.doi.org/10.3390/foods11050719
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