Oxytocin-Selective Nanogel Antibody Mimics

Oxytocin imprinted polymer nanoparticles were synthesized by glass bead supported solid phase synthesis, with NMR and molecular dynamics studies used to investigate monomer–template interactions. The nanoparticles were characterized by dynamic light scattering, scanning- and transmission electron mi...

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Detalles Bibliográficos
Autores principales: Mahajan, Rashmi, Suriyanarayanan, Subramanian, Olsson, Gustaf D., Wiklander, Jesper G., Aastrup, Teodor, Sellergren, Börje, Nicholls, Ian A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8909970/
https://www.ncbi.nlm.nih.gov/pubmed/35269677
http://dx.doi.org/10.3390/ijms23052534
Descripción
Sumario:Oxytocin imprinted polymer nanoparticles were synthesized by glass bead supported solid phase synthesis, with NMR and molecular dynamics studies used to investigate monomer–template interactions. The nanoparticles were characterized by dynamic light scattering, scanning- and transmission electron microscopy and X-ray photoelectron spectroscopy. Investigation of nanoparticle-template recognition using quartz crystal microbalance-based studies revealed sub-nanomolar affinity, k(d) ≈ 0.3 ± 0.02 nM (standard error of the mean), comparable to that of commercial polyclonal antibodies, k(d) ≈ 0.02–0.2 nM.

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