The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis

There is a lack of in vitro models able to plausibly represent the inflammation microenvironment of knee osteoarthritis (OA). We analyzed the molecules released from OA tissues (synovial membrane, cartilage, infrapatellar fat pad) and investigated whether the stimulation of human synovial fibroblast...

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Autores principales: Harvanova, Denisa, Matejova, Jana, Slovinska, Lucia, Lacko, Marek, Gulova, Slavomira, Fecskeova, Livia Kolesar, Janockova, Jana, Spakova, Timea, Rosocha, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910122/
https://www.ncbi.nlm.nih.gov/pubmed/35269618
http://dx.doi.org/10.3390/ijms23052475
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author Harvanova, Denisa
Matejova, Jana
Slovinska, Lucia
Lacko, Marek
Gulova, Slavomira
Fecskeova, Livia Kolesar
Janockova, Jana
Spakova, Timea
Rosocha, Jan
author_facet Harvanova, Denisa
Matejova, Jana
Slovinska, Lucia
Lacko, Marek
Gulova, Slavomira
Fecskeova, Livia Kolesar
Janockova, Jana
Spakova, Timea
Rosocha, Jan
author_sort Harvanova, Denisa
collection PubMed
description There is a lack of in vitro models able to plausibly represent the inflammation microenvironment of knee osteoarthritis (OA). We analyzed the molecules released from OA tissues (synovial membrane, cartilage, infrapatellar fat pad) and investigated whether the stimulation of human synovial fibroblasts (SFs), with synthetic cytokines (IL-1β and TNF-α or IFN-γ) or conditioned media (CM) from OA tissues, influence the SFs’ response, in the sense of pro-inflammatory cytokines, chemokines, growth factors, and degradative enzymes modulation. Human SFs were obtained from OA synovial membranes. SFs and their CM were analyzed for biomarkers, proliferation rate, protein profile and gene expression, before and after stimulation. Real-time PCR and multiplex assays quantified OA-related gene expression and biomolecule production. Unlike other activators, CM from OA synovial membrane (CM-SM), significantly up-regulated all genes of interest (IL-6, IL-8, MMP-1, MMP-3, RANTES, MCP-1, TSG-6, YKL-40) in SFs. Multiplex immunoassay analysis showed that levels of OA-related cytokines (IL-6, IL-8, MCP 1, IL-1Ra), chemokine (RANTES) and growth factor (VEGF), produced by CM-SM stimulated SFs, increased significantly compared to non-stimulated SFs. Molecules released from the SM from OA patients induces OA-like changes in vitro, in specific OA synovial populations (SFs). These findings promote the use and establish a compelling in vitro model that simulates the versatility and complexity of the OA disease. This model, in the future, will allow us to study new cell therapies or test drugs by reducing or avoiding animal models.
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spelling pubmed-89101222022-03-11 The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis Harvanova, Denisa Matejova, Jana Slovinska, Lucia Lacko, Marek Gulova, Slavomira Fecskeova, Livia Kolesar Janockova, Jana Spakova, Timea Rosocha, Jan Int J Mol Sci Article There is a lack of in vitro models able to plausibly represent the inflammation microenvironment of knee osteoarthritis (OA). We analyzed the molecules released from OA tissues (synovial membrane, cartilage, infrapatellar fat pad) and investigated whether the stimulation of human synovial fibroblasts (SFs), with synthetic cytokines (IL-1β and TNF-α or IFN-γ) or conditioned media (CM) from OA tissues, influence the SFs’ response, in the sense of pro-inflammatory cytokines, chemokines, growth factors, and degradative enzymes modulation. Human SFs were obtained from OA synovial membranes. SFs and their CM were analyzed for biomarkers, proliferation rate, protein profile and gene expression, before and after stimulation. Real-time PCR and multiplex assays quantified OA-related gene expression and biomolecule production. Unlike other activators, CM from OA synovial membrane (CM-SM), significantly up-regulated all genes of interest (IL-6, IL-8, MMP-1, MMP-3, RANTES, MCP-1, TSG-6, YKL-40) in SFs. Multiplex immunoassay analysis showed that levels of OA-related cytokines (IL-6, IL-8, MCP 1, IL-1Ra), chemokine (RANTES) and growth factor (VEGF), produced by CM-SM stimulated SFs, increased significantly compared to non-stimulated SFs. Molecules released from the SM from OA patients induces OA-like changes in vitro, in specific OA synovial populations (SFs). These findings promote the use and establish a compelling in vitro model that simulates the versatility and complexity of the OA disease. This model, in the future, will allow us to study new cell therapies or test drugs by reducing or avoiding animal models. MDPI 2022-02-24 /pmc/articles/PMC8910122/ /pubmed/35269618 http://dx.doi.org/10.3390/ijms23052475 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Harvanova, Denisa
Matejova, Jana
Slovinska, Lucia
Lacko, Marek
Gulova, Slavomira
Fecskeova, Livia Kolesar
Janockova, Jana
Spakova, Timea
Rosocha, Jan
The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis
title The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis
title_full The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis
title_fullStr The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis
title_full_unstemmed The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis
title_short The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis
title_sort role of synovial membrane in the development of a potential in vitro model of osteoarthritis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910122/
https://www.ncbi.nlm.nih.gov/pubmed/35269618
http://dx.doi.org/10.3390/ijms23052475
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