The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis
There is a lack of in vitro models able to plausibly represent the inflammation microenvironment of knee osteoarthritis (OA). We analyzed the molecules released from OA tissues (synovial membrane, cartilage, infrapatellar fat pad) and investigated whether the stimulation of human synovial fibroblast...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910122/ https://www.ncbi.nlm.nih.gov/pubmed/35269618 http://dx.doi.org/10.3390/ijms23052475 |
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author | Harvanova, Denisa Matejova, Jana Slovinska, Lucia Lacko, Marek Gulova, Slavomira Fecskeova, Livia Kolesar Janockova, Jana Spakova, Timea Rosocha, Jan |
author_facet | Harvanova, Denisa Matejova, Jana Slovinska, Lucia Lacko, Marek Gulova, Slavomira Fecskeova, Livia Kolesar Janockova, Jana Spakova, Timea Rosocha, Jan |
author_sort | Harvanova, Denisa |
collection | PubMed |
description | There is a lack of in vitro models able to plausibly represent the inflammation microenvironment of knee osteoarthritis (OA). We analyzed the molecules released from OA tissues (synovial membrane, cartilage, infrapatellar fat pad) and investigated whether the stimulation of human synovial fibroblasts (SFs), with synthetic cytokines (IL-1β and TNF-α or IFN-γ) or conditioned media (CM) from OA tissues, influence the SFs’ response, in the sense of pro-inflammatory cytokines, chemokines, growth factors, and degradative enzymes modulation. Human SFs were obtained from OA synovial membranes. SFs and their CM were analyzed for biomarkers, proliferation rate, protein profile and gene expression, before and after stimulation. Real-time PCR and multiplex assays quantified OA-related gene expression and biomolecule production. Unlike other activators, CM from OA synovial membrane (CM-SM), significantly up-regulated all genes of interest (IL-6, IL-8, MMP-1, MMP-3, RANTES, MCP-1, TSG-6, YKL-40) in SFs. Multiplex immunoassay analysis showed that levels of OA-related cytokines (IL-6, IL-8, MCP 1, IL-1Ra), chemokine (RANTES) and growth factor (VEGF), produced by CM-SM stimulated SFs, increased significantly compared to non-stimulated SFs. Molecules released from the SM from OA patients induces OA-like changes in vitro, in specific OA synovial populations (SFs). These findings promote the use and establish a compelling in vitro model that simulates the versatility and complexity of the OA disease. This model, in the future, will allow us to study new cell therapies or test drugs by reducing or avoiding animal models. |
format | Online Article Text |
id | pubmed-8910122 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89101222022-03-11 The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis Harvanova, Denisa Matejova, Jana Slovinska, Lucia Lacko, Marek Gulova, Slavomira Fecskeova, Livia Kolesar Janockova, Jana Spakova, Timea Rosocha, Jan Int J Mol Sci Article There is a lack of in vitro models able to plausibly represent the inflammation microenvironment of knee osteoarthritis (OA). We analyzed the molecules released from OA tissues (synovial membrane, cartilage, infrapatellar fat pad) and investigated whether the stimulation of human synovial fibroblasts (SFs), with synthetic cytokines (IL-1β and TNF-α or IFN-γ) or conditioned media (CM) from OA tissues, influence the SFs’ response, in the sense of pro-inflammatory cytokines, chemokines, growth factors, and degradative enzymes modulation. Human SFs were obtained from OA synovial membranes. SFs and their CM were analyzed for biomarkers, proliferation rate, protein profile and gene expression, before and after stimulation. Real-time PCR and multiplex assays quantified OA-related gene expression and biomolecule production. Unlike other activators, CM from OA synovial membrane (CM-SM), significantly up-regulated all genes of interest (IL-6, IL-8, MMP-1, MMP-3, RANTES, MCP-1, TSG-6, YKL-40) in SFs. Multiplex immunoassay analysis showed that levels of OA-related cytokines (IL-6, IL-8, MCP 1, IL-1Ra), chemokine (RANTES) and growth factor (VEGF), produced by CM-SM stimulated SFs, increased significantly compared to non-stimulated SFs. Molecules released from the SM from OA patients induces OA-like changes in vitro, in specific OA synovial populations (SFs). These findings promote the use and establish a compelling in vitro model that simulates the versatility and complexity of the OA disease. This model, in the future, will allow us to study new cell therapies or test drugs by reducing or avoiding animal models. MDPI 2022-02-24 /pmc/articles/PMC8910122/ /pubmed/35269618 http://dx.doi.org/10.3390/ijms23052475 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Harvanova, Denisa Matejova, Jana Slovinska, Lucia Lacko, Marek Gulova, Slavomira Fecskeova, Livia Kolesar Janockova, Jana Spakova, Timea Rosocha, Jan The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis |
title | The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis |
title_full | The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis |
title_fullStr | The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis |
title_full_unstemmed | The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis |
title_short | The Role of Synovial Membrane in the Development of a Potential In Vitro Model of Osteoarthritis |
title_sort | role of synovial membrane in the development of a potential in vitro model of osteoarthritis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910122/ https://www.ncbi.nlm.nih.gov/pubmed/35269618 http://dx.doi.org/10.3390/ijms23052475 |
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