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Manganese-Labeled Alginate Hydrogels for Image-Guided Cell Transplantation

Cell transplantation has been studied extensively as a therapeutic strategy for neurological disorders. However, to date, its effectiveness remains unsatisfactory due to low precision and efficacy of cell delivery; poor survival of transplanted cells; and inadequate monitoring of their fate in vivo....

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Autores principales: Araszkiewicz, Antonina M., Oliveira, Eduarda P., Svendsen, Terje, Drela, Katarzyna, Rogujski, Piotr, Malysz-Cymborska, Izabela, Fiedorowicz, Michal, Reis, Rui L., Oliveira, Joaquim Miguel, Walczak, Piotr, Janowski, Miroslaw, Lukomska, Barbara, Stanaszek, Luiza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910205/
https://www.ncbi.nlm.nih.gov/pubmed/35269609
http://dx.doi.org/10.3390/ijms23052465
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author Araszkiewicz, Antonina M.
Oliveira, Eduarda P.
Svendsen, Terje
Drela, Katarzyna
Rogujski, Piotr
Malysz-Cymborska, Izabela
Fiedorowicz, Michal
Reis, Rui L.
Oliveira, Joaquim Miguel
Walczak, Piotr
Janowski, Miroslaw
Lukomska, Barbara
Stanaszek, Luiza
author_facet Araszkiewicz, Antonina M.
Oliveira, Eduarda P.
Svendsen, Terje
Drela, Katarzyna
Rogujski, Piotr
Malysz-Cymborska, Izabela
Fiedorowicz, Michal
Reis, Rui L.
Oliveira, Joaquim Miguel
Walczak, Piotr
Janowski, Miroslaw
Lukomska, Barbara
Stanaszek, Luiza
author_sort Araszkiewicz, Antonina M.
collection PubMed
description Cell transplantation has been studied extensively as a therapeutic strategy for neurological disorders. However, to date, its effectiveness remains unsatisfactory due to low precision and efficacy of cell delivery; poor survival of transplanted cells; and inadequate monitoring of their fate in vivo. Fortunately, different bio-scaffolds have been proposed as cell carriers to improve the accuracy of cell delivery, survival, differentiation, and controlled release of embedded stem cells. The goal of our study was to establish hydrogel scaffolds suitable for stem cell delivery that also allow non-invasive magnetic resonance imaging (MRI). We focused on alginate-based hydrogels due to their natural origin, biocompatibility, resemblance to the extracellular matrix, and easy manipulation of gelation processes. We optimized the properties of alginate-based hydrogels, turning them into suitable carriers for transplanted cells. Human adipose-derived stem cells embedded in these hydrogels survived for at least 14 days in vitro. Alginate-based hydrogels were also modified successfully to allow their injectability via a needle. Finally, supplementing alginate hydrogels with Mn ions or Mn nanoparticles allowed for their visualization in vivo using manganese-enhanced MRI. We demonstrated that modified alginate-based hydrogels can support therapeutic cells as MRI-detectable matrices.
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spelling pubmed-89102052022-03-11 Manganese-Labeled Alginate Hydrogels for Image-Guided Cell Transplantation Araszkiewicz, Antonina M. Oliveira, Eduarda P. Svendsen, Terje Drela, Katarzyna Rogujski, Piotr Malysz-Cymborska, Izabela Fiedorowicz, Michal Reis, Rui L. Oliveira, Joaquim Miguel Walczak, Piotr Janowski, Miroslaw Lukomska, Barbara Stanaszek, Luiza Int J Mol Sci Article Cell transplantation has been studied extensively as a therapeutic strategy for neurological disorders. However, to date, its effectiveness remains unsatisfactory due to low precision and efficacy of cell delivery; poor survival of transplanted cells; and inadequate monitoring of their fate in vivo. Fortunately, different bio-scaffolds have been proposed as cell carriers to improve the accuracy of cell delivery, survival, differentiation, and controlled release of embedded stem cells. The goal of our study was to establish hydrogel scaffolds suitable for stem cell delivery that also allow non-invasive magnetic resonance imaging (MRI). We focused on alginate-based hydrogels due to their natural origin, biocompatibility, resemblance to the extracellular matrix, and easy manipulation of gelation processes. We optimized the properties of alginate-based hydrogels, turning them into suitable carriers for transplanted cells. Human adipose-derived stem cells embedded in these hydrogels survived for at least 14 days in vitro. Alginate-based hydrogels were also modified successfully to allow their injectability via a needle. Finally, supplementing alginate hydrogels with Mn ions or Mn nanoparticles allowed for their visualization in vivo using manganese-enhanced MRI. We demonstrated that modified alginate-based hydrogels can support therapeutic cells as MRI-detectable matrices. MDPI 2022-02-23 /pmc/articles/PMC8910205/ /pubmed/35269609 http://dx.doi.org/10.3390/ijms23052465 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Araszkiewicz, Antonina M.
Oliveira, Eduarda P.
Svendsen, Terje
Drela, Katarzyna
Rogujski, Piotr
Malysz-Cymborska, Izabela
Fiedorowicz, Michal
Reis, Rui L.
Oliveira, Joaquim Miguel
Walczak, Piotr
Janowski, Miroslaw
Lukomska, Barbara
Stanaszek, Luiza
Manganese-Labeled Alginate Hydrogels for Image-Guided Cell Transplantation
title Manganese-Labeled Alginate Hydrogels for Image-Guided Cell Transplantation
title_full Manganese-Labeled Alginate Hydrogels for Image-Guided Cell Transplantation
title_fullStr Manganese-Labeled Alginate Hydrogels for Image-Guided Cell Transplantation
title_full_unstemmed Manganese-Labeled Alginate Hydrogels for Image-Guided Cell Transplantation
title_short Manganese-Labeled Alginate Hydrogels for Image-Guided Cell Transplantation
title_sort manganese-labeled alginate hydrogels for image-guided cell transplantation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910205/
https://www.ncbi.nlm.nih.gov/pubmed/35269609
http://dx.doi.org/10.3390/ijms23052465
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