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Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize

Tassel branch number (TBN) is one of the important agronomic traits that contribute to the efficiency of seed production and has been selected strongly during the modern maize breeding process. However, the genetic mechanisms of TBN in maize are not entirely clear. In this study, we used a B73 × CML...

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Autores principales: Guan, Honghui, Chen, Xiaojing, Wang, Kailiang, Liu, Xuyang, Zhang, Dengfeng, Li, Yongxiang, Song, Yanchun, Shi, Yunsu, Wang, Tianyu, Li, Chunhui, Li, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910302/
https://www.ncbi.nlm.nih.gov/pubmed/35269730
http://dx.doi.org/10.3390/ijms23052586
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author Guan, Honghui
Chen, Xiaojing
Wang, Kailiang
Liu, Xuyang
Zhang, Dengfeng
Li, Yongxiang
Song, Yanchun
Shi, Yunsu
Wang, Tianyu
Li, Chunhui
Li, Yu
author_facet Guan, Honghui
Chen, Xiaojing
Wang, Kailiang
Liu, Xuyang
Zhang, Dengfeng
Li, Yongxiang
Song, Yanchun
Shi, Yunsu
Wang, Tianyu
Li, Chunhui
Li, Yu
author_sort Guan, Honghui
collection PubMed
description Tassel branch number (TBN) is one of the important agronomic traits that contribute to the efficiency of seed production and has been selected strongly during the modern maize breeding process. However, the genetic mechanisms of TBN in maize are not entirely clear. In this study, we used a B73 × CML247 recombination inbred lines (RILs) population to detect quantitative trait loci (QTLs) for TBN. A total of four QTLs (qTBN2a, qTBN2b, qTBN4, and qTBN6) and six candidate genes were identified through expression analysis. Further, one of the candidates (GRMZM2G010011, ZmPAT7) encoding an S-acyltransferase was selected to validate its function by CRISPR-Cas9 technology, and its loss-of-function lines showed a significant increase in TBN. A key SNP(−101) variation in the promoter of ZmPAT7 was significantly associated with TBN. A total of 17 distant eQTLs associated with the expression of ZmPAT7 were identified in expression quantitative trait loci (eQTL) analysis, and ZmNAC3 may be a major factor involved in regulating ZmPAT7. These findings of the present study promote our understanding of the genetic basis of tassel architecture and provide new gene resources for maize breeding improvement.
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spelling pubmed-89103022022-03-11 Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize Guan, Honghui Chen, Xiaojing Wang, Kailiang Liu, Xuyang Zhang, Dengfeng Li, Yongxiang Song, Yanchun Shi, Yunsu Wang, Tianyu Li, Chunhui Li, Yu Int J Mol Sci Article Tassel branch number (TBN) is one of the important agronomic traits that contribute to the efficiency of seed production and has been selected strongly during the modern maize breeding process. However, the genetic mechanisms of TBN in maize are not entirely clear. In this study, we used a B73 × CML247 recombination inbred lines (RILs) population to detect quantitative trait loci (QTLs) for TBN. A total of four QTLs (qTBN2a, qTBN2b, qTBN4, and qTBN6) and six candidate genes were identified through expression analysis. Further, one of the candidates (GRMZM2G010011, ZmPAT7) encoding an S-acyltransferase was selected to validate its function by CRISPR-Cas9 technology, and its loss-of-function lines showed a significant increase in TBN. A key SNP(−101) variation in the promoter of ZmPAT7 was significantly associated with TBN. A total of 17 distant eQTLs associated with the expression of ZmPAT7 were identified in expression quantitative trait loci (eQTL) analysis, and ZmNAC3 may be a major factor involved in regulating ZmPAT7. These findings of the present study promote our understanding of the genetic basis of tassel architecture and provide new gene resources for maize breeding improvement. MDPI 2022-02-26 /pmc/articles/PMC8910302/ /pubmed/35269730 http://dx.doi.org/10.3390/ijms23052586 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guan, Honghui
Chen, Xiaojing
Wang, Kailiang
Liu, Xuyang
Zhang, Dengfeng
Li, Yongxiang
Song, Yanchun
Shi, Yunsu
Wang, Tianyu
Li, Chunhui
Li, Yu
Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize
title Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize
title_full Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize
title_fullStr Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize
title_full_unstemmed Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize
title_short Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize
title_sort genetic variation in zmpat7 contributes to tassel branch number in maize
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910302/
https://www.ncbi.nlm.nih.gov/pubmed/35269730
http://dx.doi.org/10.3390/ijms23052586
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