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Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize
Tassel branch number (TBN) is one of the important agronomic traits that contribute to the efficiency of seed production and has been selected strongly during the modern maize breeding process. However, the genetic mechanisms of TBN in maize are not entirely clear. In this study, we used a B73 × CML...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910302/ https://www.ncbi.nlm.nih.gov/pubmed/35269730 http://dx.doi.org/10.3390/ijms23052586 |
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author | Guan, Honghui Chen, Xiaojing Wang, Kailiang Liu, Xuyang Zhang, Dengfeng Li, Yongxiang Song, Yanchun Shi, Yunsu Wang, Tianyu Li, Chunhui Li, Yu |
author_facet | Guan, Honghui Chen, Xiaojing Wang, Kailiang Liu, Xuyang Zhang, Dengfeng Li, Yongxiang Song, Yanchun Shi, Yunsu Wang, Tianyu Li, Chunhui Li, Yu |
author_sort | Guan, Honghui |
collection | PubMed |
description | Tassel branch number (TBN) is one of the important agronomic traits that contribute to the efficiency of seed production and has been selected strongly during the modern maize breeding process. However, the genetic mechanisms of TBN in maize are not entirely clear. In this study, we used a B73 × CML247 recombination inbred lines (RILs) population to detect quantitative trait loci (QTLs) for TBN. A total of four QTLs (qTBN2a, qTBN2b, qTBN4, and qTBN6) and six candidate genes were identified through expression analysis. Further, one of the candidates (GRMZM2G010011, ZmPAT7) encoding an S-acyltransferase was selected to validate its function by CRISPR-Cas9 technology, and its loss-of-function lines showed a significant increase in TBN. A key SNP(−101) variation in the promoter of ZmPAT7 was significantly associated with TBN. A total of 17 distant eQTLs associated with the expression of ZmPAT7 were identified in expression quantitative trait loci (eQTL) analysis, and ZmNAC3 may be a major factor involved in regulating ZmPAT7. These findings of the present study promote our understanding of the genetic basis of tassel architecture and provide new gene resources for maize breeding improvement. |
format | Online Article Text |
id | pubmed-8910302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89103022022-03-11 Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize Guan, Honghui Chen, Xiaojing Wang, Kailiang Liu, Xuyang Zhang, Dengfeng Li, Yongxiang Song, Yanchun Shi, Yunsu Wang, Tianyu Li, Chunhui Li, Yu Int J Mol Sci Article Tassel branch number (TBN) is one of the important agronomic traits that contribute to the efficiency of seed production and has been selected strongly during the modern maize breeding process. However, the genetic mechanisms of TBN in maize are not entirely clear. In this study, we used a B73 × CML247 recombination inbred lines (RILs) population to detect quantitative trait loci (QTLs) for TBN. A total of four QTLs (qTBN2a, qTBN2b, qTBN4, and qTBN6) and six candidate genes were identified through expression analysis. Further, one of the candidates (GRMZM2G010011, ZmPAT7) encoding an S-acyltransferase was selected to validate its function by CRISPR-Cas9 technology, and its loss-of-function lines showed a significant increase in TBN. A key SNP(−101) variation in the promoter of ZmPAT7 was significantly associated with TBN. A total of 17 distant eQTLs associated with the expression of ZmPAT7 were identified in expression quantitative trait loci (eQTL) analysis, and ZmNAC3 may be a major factor involved in regulating ZmPAT7. These findings of the present study promote our understanding of the genetic basis of tassel architecture and provide new gene resources for maize breeding improvement. MDPI 2022-02-26 /pmc/articles/PMC8910302/ /pubmed/35269730 http://dx.doi.org/10.3390/ijms23052586 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guan, Honghui Chen, Xiaojing Wang, Kailiang Liu, Xuyang Zhang, Dengfeng Li, Yongxiang Song, Yanchun Shi, Yunsu Wang, Tianyu Li, Chunhui Li, Yu Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize |
title | Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize |
title_full | Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize |
title_fullStr | Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize |
title_full_unstemmed | Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize |
title_short | Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize |
title_sort | genetic variation in zmpat7 contributes to tassel branch number in maize |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910302/ https://www.ncbi.nlm.nih.gov/pubmed/35269730 http://dx.doi.org/10.3390/ijms23052586 |
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