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Plant Peroxisome-Targeting Effector MoPtep1 Is Required for the Virulence of Magnaporthe oryzae

Rice blast caused by Magnaporthe oryzae is one of the most serious fungous diseases in rice. In the past decades, studies have reported that numerous M. oryzae effectors were secreted into plant cells to facilitate inoculation. Effectors target host proteins to assist the virulence of pathogens via...

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Autores principales: Ning, Na, Xie, Xin, Yu, Haiyue, Mei, Jie, Li, Qianqian, Zuo, Shimin, Wu, Hanxiang, Liu, Wende, Li, Zhiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910473/
https://www.ncbi.nlm.nih.gov/pubmed/35269662
http://dx.doi.org/10.3390/ijms23052515
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author Ning, Na
Xie, Xin
Yu, Haiyue
Mei, Jie
Li, Qianqian
Zuo, Shimin
Wu, Hanxiang
Liu, Wende
Li, Zhiqiang
author_facet Ning, Na
Xie, Xin
Yu, Haiyue
Mei, Jie
Li, Qianqian
Zuo, Shimin
Wu, Hanxiang
Liu, Wende
Li, Zhiqiang
author_sort Ning, Na
collection PubMed
description Rice blast caused by Magnaporthe oryzae is one of the most serious fungous diseases in rice. In the past decades, studies have reported that numerous M. oryzae effectors were secreted into plant cells to facilitate inoculation. Effectors target host proteins to assist the virulence of pathogens via the localization of specific organelles, such as the nucleus, endoplasmic reticulum, chloroplast, etc. However, studies on the pathogenesis of peroxisome-targeting effectors are still limited. In our previous study, we analyzed the subcellular localization of candidate effectors from M. oryzae using the agrobacterium-mediated transient expression system in tobacco and found that MoPtep1 (peroxisomes-targeted effector protein 1) localized in plant peroxisomes. Here, we proved that MoPtep1 was induced in the early stage of the M. oryzae infection and positively regulated the pathogenicity, while it did not affect the vegetative growth of mycelia. Subcellular localization results showed that MoPtep1 was localized in the plant peroxisomes with a signal peptide and a cupredoxin domain. Sequence analysis indicated that the homologous protein of MoPtep1 in plant-pathogenic fungi was evolutionarily conserved. Furthermore, MoPtep1 could suppress INF1-induced cell death in tobacco, and the targeting host proteins were identified using the Y2H system. Our results suggested that MoPtep1 is an important pathogenic effector in rice blast.
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spelling pubmed-89104732022-03-11 Plant Peroxisome-Targeting Effector MoPtep1 Is Required for the Virulence of Magnaporthe oryzae Ning, Na Xie, Xin Yu, Haiyue Mei, Jie Li, Qianqian Zuo, Shimin Wu, Hanxiang Liu, Wende Li, Zhiqiang Int J Mol Sci Article Rice blast caused by Magnaporthe oryzae is one of the most serious fungous diseases in rice. In the past decades, studies have reported that numerous M. oryzae effectors were secreted into plant cells to facilitate inoculation. Effectors target host proteins to assist the virulence of pathogens via the localization of specific organelles, such as the nucleus, endoplasmic reticulum, chloroplast, etc. However, studies on the pathogenesis of peroxisome-targeting effectors are still limited. In our previous study, we analyzed the subcellular localization of candidate effectors from M. oryzae using the agrobacterium-mediated transient expression system in tobacco and found that MoPtep1 (peroxisomes-targeted effector protein 1) localized in plant peroxisomes. Here, we proved that MoPtep1 was induced in the early stage of the M. oryzae infection and positively regulated the pathogenicity, while it did not affect the vegetative growth of mycelia. Subcellular localization results showed that MoPtep1 was localized in the plant peroxisomes with a signal peptide and a cupredoxin domain. Sequence analysis indicated that the homologous protein of MoPtep1 in plant-pathogenic fungi was evolutionarily conserved. Furthermore, MoPtep1 could suppress INF1-induced cell death in tobacco, and the targeting host proteins were identified using the Y2H system. Our results suggested that MoPtep1 is an important pathogenic effector in rice blast. MDPI 2022-02-24 /pmc/articles/PMC8910473/ /pubmed/35269662 http://dx.doi.org/10.3390/ijms23052515 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ning, Na
Xie, Xin
Yu, Haiyue
Mei, Jie
Li, Qianqian
Zuo, Shimin
Wu, Hanxiang
Liu, Wende
Li, Zhiqiang
Plant Peroxisome-Targeting Effector MoPtep1 Is Required for the Virulence of Magnaporthe oryzae
title Plant Peroxisome-Targeting Effector MoPtep1 Is Required for the Virulence of Magnaporthe oryzae
title_full Plant Peroxisome-Targeting Effector MoPtep1 Is Required for the Virulence of Magnaporthe oryzae
title_fullStr Plant Peroxisome-Targeting Effector MoPtep1 Is Required for the Virulence of Magnaporthe oryzae
title_full_unstemmed Plant Peroxisome-Targeting Effector MoPtep1 Is Required for the Virulence of Magnaporthe oryzae
title_short Plant Peroxisome-Targeting Effector MoPtep1 Is Required for the Virulence of Magnaporthe oryzae
title_sort plant peroxisome-targeting effector moptep1 is required for the virulence of magnaporthe oryzae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910473/
https://www.ncbi.nlm.nih.gov/pubmed/35269662
http://dx.doi.org/10.3390/ijms23052515
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