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Distinct Firing Activities of the Hypothalamic Arcuate Nucleus Neurons to Appetite Hormones

The hypothalamic arcuate nucleus (Arc) is a central unit that controls the appetite through the integration of metabolic, hormonal, and neuronal afferent inputs. Agouti-related protein (AgRP), proopiomelanocortin (POMC), and dopaminergic neurons in the Arc differentially regulate feeding behaviors i...

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Autores principales: Na, Junewoo, Park, Byong Seo, Jang, Doohyeong, Kim, Donggue, Tu, Thai Hien, Ryu, Youngjae, Ha, Chang Man, Koch, Marco, Yang, Sungchil, Kim, Jae Geun, Yang, Sunggu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910626/
https://www.ncbi.nlm.nih.gov/pubmed/35269751
http://dx.doi.org/10.3390/ijms23052609
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author Na, Junewoo
Park, Byong Seo
Jang, Doohyeong
Kim, Donggue
Tu, Thai Hien
Ryu, Youngjae
Ha, Chang Man
Koch, Marco
Yang, Sungchil
Kim, Jae Geun
Yang, Sunggu
author_facet Na, Junewoo
Park, Byong Seo
Jang, Doohyeong
Kim, Donggue
Tu, Thai Hien
Ryu, Youngjae
Ha, Chang Man
Koch, Marco
Yang, Sungchil
Kim, Jae Geun
Yang, Sunggu
author_sort Na, Junewoo
collection PubMed
description The hypothalamic arcuate nucleus (Arc) is a central unit that controls the appetite through the integration of metabolic, hormonal, and neuronal afferent inputs. Agouti-related protein (AgRP), proopiomelanocortin (POMC), and dopaminergic neurons in the Arc differentially regulate feeding behaviors in response to hunger, satiety, and appetite, respectively. At the time of writing, the anatomical and electrophysiological characterization of these three neurons has not yet been intensively explored. Here, we interrogated the overall characterization of AgRP, POMC, and dopaminergic neurons using genetic mouse models, immunohistochemistry, and whole-cell patch recordings. We identified the distinct geographical location and intrinsic properties of each neuron in the Arc with the transgenic lines labelled with cell-specific reporter proteins. Moreover, AgRP, POMC, and dopaminergic neurons had different firing activities to ghrelin and leptin treatments. Ghrelin led to the increased firing rate of dopaminergic and AgRP neurons, and the decreased firing rate of POMC. In sharp contrast, leptin resulted in the decreased firing rate of AgRP neurons and the increased firing rate of POMC neurons, while it did not change the firing rate of dopaminergic neurons in Arc. These findings demonstrate the anatomical and physiological uniqueness of three hypothalamic Arc neurons to appetite control.
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spelling pubmed-89106262022-03-11 Distinct Firing Activities of the Hypothalamic Arcuate Nucleus Neurons to Appetite Hormones Na, Junewoo Park, Byong Seo Jang, Doohyeong Kim, Donggue Tu, Thai Hien Ryu, Youngjae Ha, Chang Man Koch, Marco Yang, Sungchil Kim, Jae Geun Yang, Sunggu Int J Mol Sci Article The hypothalamic arcuate nucleus (Arc) is a central unit that controls the appetite through the integration of metabolic, hormonal, and neuronal afferent inputs. Agouti-related protein (AgRP), proopiomelanocortin (POMC), and dopaminergic neurons in the Arc differentially regulate feeding behaviors in response to hunger, satiety, and appetite, respectively. At the time of writing, the anatomical and electrophysiological characterization of these three neurons has not yet been intensively explored. Here, we interrogated the overall characterization of AgRP, POMC, and dopaminergic neurons using genetic mouse models, immunohistochemistry, and whole-cell patch recordings. We identified the distinct geographical location and intrinsic properties of each neuron in the Arc with the transgenic lines labelled with cell-specific reporter proteins. Moreover, AgRP, POMC, and dopaminergic neurons had different firing activities to ghrelin and leptin treatments. Ghrelin led to the increased firing rate of dopaminergic and AgRP neurons, and the decreased firing rate of POMC. In sharp contrast, leptin resulted in the decreased firing rate of AgRP neurons and the increased firing rate of POMC neurons, while it did not change the firing rate of dopaminergic neurons in Arc. These findings demonstrate the anatomical and physiological uniqueness of three hypothalamic Arc neurons to appetite control. MDPI 2022-02-26 /pmc/articles/PMC8910626/ /pubmed/35269751 http://dx.doi.org/10.3390/ijms23052609 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Na, Junewoo
Park, Byong Seo
Jang, Doohyeong
Kim, Donggue
Tu, Thai Hien
Ryu, Youngjae
Ha, Chang Man
Koch, Marco
Yang, Sungchil
Kim, Jae Geun
Yang, Sunggu
Distinct Firing Activities of the Hypothalamic Arcuate Nucleus Neurons to Appetite Hormones
title Distinct Firing Activities of the Hypothalamic Arcuate Nucleus Neurons to Appetite Hormones
title_full Distinct Firing Activities of the Hypothalamic Arcuate Nucleus Neurons to Appetite Hormones
title_fullStr Distinct Firing Activities of the Hypothalamic Arcuate Nucleus Neurons to Appetite Hormones
title_full_unstemmed Distinct Firing Activities of the Hypothalamic Arcuate Nucleus Neurons to Appetite Hormones
title_short Distinct Firing Activities of the Hypothalamic Arcuate Nucleus Neurons to Appetite Hormones
title_sort distinct firing activities of the hypothalamic arcuate nucleus neurons to appetite hormones
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910626/
https://www.ncbi.nlm.nih.gov/pubmed/35269751
http://dx.doi.org/10.3390/ijms23052609
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