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Characterization and Identification of a Ripening-Related Gene AaPG18 in Actinidia arguta

Actinidia arguta (A. arguta) is a kind of climacteric fruit that quickly softens and limits fruit shelf-life and commercial value. Therefore, it is of great significance to develop kiwifruit genotypes with an extended shelf-life of fruit. However, the ripening and softening mechanisms remain unclear...

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Autores principales: Li, Yukuo, Huang, Hailei, Abid, Muhammad, Gu, Hong, Fang, Jinbao, Cheng, Zhongping, Qi, Xiujuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910643/
https://www.ncbi.nlm.nih.gov/pubmed/35269737
http://dx.doi.org/10.3390/ijms23052597
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author Li, Yukuo
Huang, Hailei
Abid, Muhammad
Gu, Hong
Fang, Jinbao
Cheng, Zhongping
Qi, Xiujuan
author_facet Li, Yukuo
Huang, Hailei
Abid, Muhammad
Gu, Hong
Fang, Jinbao
Cheng, Zhongping
Qi, Xiujuan
author_sort Li, Yukuo
collection PubMed
description Actinidia arguta (A. arguta) is a kind of climacteric fruit that quickly softens and limits fruit shelf-life and commercial value. Therefore, it is of great significance to develop kiwifruit genotypes with an extended shelf-life of fruit. However, the ripening and softening mechanisms remain unclear in A. arguta. Here, we demonstrated that a key polygalacturonase (PG)-encoding gene AaPG18 was involved in A. arguta ripening through the degradation of the cell wall. Fruits were harvested at three developmental stages (S1, S2, and S3) for high-throughput transcriptome sequencing, based on which two candidate transcripts c109562_g1 and c111961_g1 were screened. The genome-wide identification of the PG gene family assigned c109562_g1 and c111961_g1 to correspond to AaPG4 and AaPG18, respectively. The expression profiles of candidate genes at six preharvest stages of fruit showed significantly higher expression levels of AaPG18 than AaPG4, indicating AaPG18 might be a key gene during fruit ripening processes. The subcellular localization displayed AaPG18 was located at the cytoplasmic membrane. The transient overexpression of AaPG18 in strawberry and the following morphological observation suggested AaPG18 played a key role in maintaining the stability of cell morphology. The homologous transient transformation in A. arguta “RB-4” proved the crucial function of AaPG18 in fruit ripening processes by causing the rapid redness of the fruit, which was an indicator of fruit maturity. All in all, our results identified AaPG18 as a key candidate gene involved in cell wall degeneration, which provides a basis for the subsequent exploration of the molecular mechanisms underlying the ripening and softening of A. arguta fruit.
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spelling pubmed-89106432022-03-11 Characterization and Identification of a Ripening-Related Gene AaPG18 in Actinidia arguta Li, Yukuo Huang, Hailei Abid, Muhammad Gu, Hong Fang, Jinbao Cheng, Zhongping Qi, Xiujuan Int J Mol Sci Article Actinidia arguta (A. arguta) is a kind of climacteric fruit that quickly softens and limits fruit shelf-life and commercial value. Therefore, it is of great significance to develop kiwifruit genotypes with an extended shelf-life of fruit. However, the ripening and softening mechanisms remain unclear in A. arguta. Here, we demonstrated that a key polygalacturonase (PG)-encoding gene AaPG18 was involved in A. arguta ripening through the degradation of the cell wall. Fruits were harvested at three developmental stages (S1, S2, and S3) for high-throughput transcriptome sequencing, based on which two candidate transcripts c109562_g1 and c111961_g1 were screened. The genome-wide identification of the PG gene family assigned c109562_g1 and c111961_g1 to correspond to AaPG4 and AaPG18, respectively. The expression profiles of candidate genes at six preharvest stages of fruit showed significantly higher expression levels of AaPG18 than AaPG4, indicating AaPG18 might be a key gene during fruit ripening processes. The subcellular localization displayed AaPG18 was located at the cytoplasmic membrane. The transient overexpression of AaPG18 in strawberry and the following morphological observation suggested AaPG18 played a key role in maintaining the stability of cell morphology. The homologous transient transformation in A. arguta “RB-4” proved the crucial function of AaPG18 in fruit ripening processes by causing the rapid redness of the fruit, which was an indicator of fruit maturity. All in all, our results identified AaPG18 as a key candidate gene involved in cell wall degeneration, which provides a basis for the subsequent exploration of the molecular mechanisms underlying the ripening and softening of A. arguta fruit. MDPI 2022-02-26 /pmc/articles/PMC8910643/ /pubmed/35269737 http://dx.doi.org/10.3390/ijms23052597 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Yukuo
Huang, Hailei
Abid, Muhammad
Gu, Hong
Fang, Jinbao
Cheng, Zhongping
Qi, Xiujuan
Characterization and Identification of a Ripening-Related Gene AaPG18 in Actinidia arguta
title Characterization and Identification of a Ripening-Related Gene AaPG18 in Actinidia arguta
title_full Characterization and Identification of a Ripening-Related Gene AaPG18 in Actinidia arguta
title_fullStr Characterization and Identification of a Ripening-Related Gene AaPG18 in Actinidia arguta
title_full_unstemmed Characterization and Identification of a Ripening-Related Gene AaPG18 in Actinidia arguta
title_short Characterization and Identification of a Ripening-Related Gene AaPG18 in Actinidia arguta
title_sort characterization and identification of a ripening-related gene aapg18 in actinidia arguta
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910643/
https://www.ncbi.nlm.nih.gov/pubmed/35269737
http://dx.doi.org/10.3390/ijms23052597
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