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Association of the Unstimulated Whole Salivary Cytokine IL-1β Levels with Initial, Moderate and Severe Periodontitis. A Case Control Study

Periodontitis (P) is a highly prevalent inflammatory disease of the oral cavity. The objective of the study was to evaluate the stages of pro-inflammatory cytokine IL-1β in initial, moderate and severe periodontitis. One hundred and twenty two patients were included in the study. Periodontitis subje...

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Autores principales: Majeed, Muhammad M., Ahmed, Imtiaz, Roome, Talat, Alali, Yasser, Al-Aali, Khulud A., Ahmed, Naseer, Saleem, Zohra, Alhumaidan, Abdulkareem A., Farooqui, Waqas A., Ahmed, Saeeda, Vohra, Fahim, Abduljabbar, Tariq
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910701/
https://www.ncbi.nlm.nih.gov/pubmed/35270581
http://dx.doi.org/10.3390/ijerph19052889
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author Majeed, Muhammad M.
Ahmed, Imtiaz
Roome, Talat
Alali, Yasser
Al-Aali, Khulud A.
Ahmed, Naseer
Saleem, Zohra
Alhumaidan, Abdulkareem A.
Farooqui, Waqas A.
Ahmed, Saeeda
Vohra, Fahim
Abduljabbar, Tariq
author_facet Majeed, Muhammad M.
Ahmed, Imtiaz
Roome, Talat
Alali, Yasser
Al-Aali, Khulud A.
Ahmed, Naseer
Saleem, Zohra
Alhumaidan, Abdulkareem A.
Farooqui, Waqas A.
Ahmed, Saeeda
Vohra, Fahim
Abduljabbar, Tariq
author_sort Majeed, Muhammad M.
collection PubMed
description Periodontitis (P) is a highly prevalent inflammatory disease of the oral cavity. The objective of the study was to evaluate the stages of pro-inflammatory cytokine IL-1β in initial, moderate and severe periodontitis. One hundred and twenty two patients were included in the study. Periodontitis subjects had at least 20 natural teeth and ≥8 sites with pocket depths of >4 mm and clinical attachment loss (CAL). A questionnaire was used with respect to the socio demographic parameters which included age, gender, ethnicity, education, marital, residence and occupation. To categorize the severity of the disease, teeth were assessed for, Plaque index (PI), Bleeding on probing (BOP), CAL, missing tooth, tooth mobility and bone loss. Unstimulated whole saliva (UWS) was collected and Interleukin-1β (IL-1β) cytokine levels were analyzed using enzyme linked immunosorbent assay with microplate reader at 450 nm. Clinical parameters and salivary cytokine concentrations were assessed using one-way analysis of variance, whereas a correlation of cases with gender and severity of periodontitis was evaluated using chi-square test. Fifty-nine patients were healthy controls and 63 were periodontitis patients Thirty two percent (n = 20) had initial periodontitis, 40% (n = 25) suffered from moderate and 29% (n = 18) had severe periodontitis. Periodontitis subgroups were significantly different with regards to age and gender (p < 0.001). The mean PPD and CAL among the periodontitis patients (PPD, 3.52 ± 1.25 mm; CAL, 4.04 ± 1.64 mm) were significantly compromised (p < 0.05) compared to healthy controls (PPD, 1.52 ± 0.73 mm; CAL, 0.08 ± 0.28 mm). Increased levels of IL-1β were associated with high CAL and PPD findings. UWS IL-1β levels were higher in periodontitis patients compared to healthy individuals. In addition, cases of severe periodontitis showed significantly higher UWS IL-1β levels compared to initial and moderate periodontitis patients. Comparative levels of salivary IL-1β can be potentially used as a diagnostic tool for periodontitis identification and disease progression along with clinical parameters.
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spelling pubmed-89107012022-03-11 Association of the Unstimulated Whole Salivary Cytokine IL-1β Levels with Initial, Moderate and Severe Periodontitis. A Case Control Study Majeed, Muhammad M. Ahmed, Imtiaz Roome, Talat Alali, Yasser Al-Aali, Khulud A. Ahmed, Naseer Saleem, Zohra Alhumaidan, Abdulkareem A. Farooqui, Waqas A. Ahmed, Saeeda Vohra, Fahim Abduljabbar, Tariq Int J Environ Res Public Health Article Periodontitis (P) is a highly prevalent inflammatory disease of the oral cavity. The objective of the study was to evaluate the stages of pro-inflammatory cytokine IL-1β in initial, moderate and severe periodontitis. One hundred and twenty two patients were included in the study. Periodontitis subjects had at least 20 natural teeth and ≥8 sites with pocket depths of >4 mm and clinical attachment loss (CAL). A questionnaire was used with respect to the socio demographic parameters which included age, gender, ethnicity, education, marital, residence and occupation. To categorize the severity of the disease, teeth were assessed for, Plaque index (PI), Bleeding on probing (BOP), CAL, missing tooth, tooth mobility and bone loss. Unstimulated whole saliva (UWS) was collected and Interleukin-1β (IL-1β) cytokine levels were analyzed using enzyme linked immunosorbent assay with microplate reader at 450 nm. Clinical parameters and salivary cytokine concentrations were assessed using one-way analysis of variance, whereas a correlation of cases with gender and severity of periodontitis was evaluated using chi-square test. Fifty-nine patients were healthy controls and 63 were periodontitis patients Thirty two percent (n = 20) had initial periodontitis, 40% (n = 25) suffered from moderate and 29% (n = 18) had severe periodontitis. Periodontitis subgroups were significantly different with regards to age and gender (p < 0.001). The mean PPD and CAL among the periodontitis patients (PPD, 3.52 ± 1.25 mm; CAL, 4.04 ± 1.64 mm) were significantly compromised (p < 0.05) compared to healthy controls (PPD, 1.52 ± 0.73 mm; CAL, 0.08 ± 0.28 mm). Increased levels of IL-1β were associated with high CAL and PPD findings. UWS IL-1β levels were higher in periodontitis patients compared to healthy individuals. In addition, cases of severe periodontitis showed significantly higher UWS IL-1β levels compared to initial and moderate periodontitis patients. Comparative levels of salivary IL-1β can be potentially used as a diagnostic tool for periodontitis identification and disease progression along with clinical parameters. MDPI 2022-03-02 /pmc/articles/PMC8910701/ /pubmed/35270581 http://dx.doi.org/10.3390/ijerph19052889 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Majeed, Muhammad M.
Ahmed, Imtiaz
Roome, Talat
Alali, Yasser
Al-Aali, Khulud A.
Ahmed, Naseer
Saleem, Zohra
Alhumaidan, Abdulkareem A.
Farooqui, Waqas A.
Ahmed, Saeeda
Vohra, Fahim
Abduljabbar, Tariq
Association of the Unstimulated Whole Salivary Cytokine IL-1β Levels with Initial, Moderate and Severe Periodontitis. A Case Control Study
title Association of the Unstimulated Whole Salivary Cytokine IL-1β Levels with Initial, Moderate and Severe Periodontitis. A Case Control Study
title_full Association of the Unstimulated Whole Salivary Cytokine IL-1β Levels with Initial, Moderate and Severe Periodontitis. A Case Control Study
title_fullStr Association of the Unstimulated Whole Salivary Cytokine IL-1β Levels with Initial, Moderate and Severe Periodontitis. A Case Control Study
title_full_unstemmed Association of the Unstimulated Whole Salivary Cytokine IL-1β Levels with Initial, Moderate and Severe Periodontitis. A Case Control Study
title_short Association of the Unstimulated Whole Salivary Cytokine IL-1β Levels with Initial, Moderate and Severe Periodontitis. A Case Control Study
title_sort association of the unstimulated whole salivary cytokine il-1β levels with initial, moderate and severe periodontitis. a case control study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910701/
https://www.ncbi.nlm.nih.gov/pubmed/35270581
http://dx.doi.org/10.3390/ijerph19052889
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