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CPR Gene Contributes to Integument Function and Ovary Development in a Rice Planthopper

Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) is an essential enzyme that transfers electrons from NADPH to cytochrome P450 monooxygenases. CPR is involved in cuticular hydrocarbon (CHC) synthesis in insects and is vital for insect development and survival. Here...

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Autores principales: Wang, Zhe-Chao, Tao, Shuai, Cheng, Xu, Li, Dan-Ting, Zhang, Chuan-Xi, Bao, Yan-Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910901/
https://www.ncbi.nlm.nih.gov/pubmed/35270018
http://dx.doi.org/10.3390/ijms23052875
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author Wang, Zhe-Chao
Tao, Shuai
Cheng, Xu
Li, Dan-Ting
Zhang, Chuan-Xi
Bao, Yan-Yuan
author_facet Wang, Zhe-Chao
Tao, Shuai
Cheng, Xu
Li, Dan-Ting
Zhang, Chuan-Xi
Bao, Yan-Yuan
author_sort Wang, Zhe-Chao
collection PubMed
description Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) is an essential enzyme that transfers electrons from NADPH to cytochrome P450 monooxygenases. CPR is involved in cuticular hydrocarbon (CHC) synthesis in insects and is vital for insect development and survival. Here, we clarify the physiological function of a CPR gene in Nilaparvata lugens, an important rice pest, by using RNA interference. CPR gene knockdown leads to the functional loss of waterproofing and water retention in the integument of female adults, which causes significantly reduced body weight and a lethal phenotype. Scanning electron microscopy shows that the lipid layer on the outermost surface of the abdominal cuticle becomes thin in dsCPR-injected adults. Furthermore, CHC profile analysis reveals that CPR knockdown significantly decreases the contents of CHCs with a carbon chain length ≥ C27 in adult females. Moreover, we find that CPR knockdown generates a deficient phenotype in ovaries with deformed oocytes and a complete failure of egg-laying. These findings suggest that CPR plays multiple functional roles in CHC biosynthesis and embryo development in insects.
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spelling pubmed-89109012022-03-11 CPR Gene Contributes to Integument Function and Ovary Development in a Rice Planthopper Wang, Zhe-Chao Tao, Shuai Cheng, Xu Li, Dan-Ting Zhang, Chuan-Xi Bao, Yan-Yuan Int J Mol Sci Article Nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P450 reductase (CPR) is an essential enzyme that transfers electrons from NADPH to cytochrome P450 monooxygenases. CPR is involved in cuticular hydrocarbon (CHC) synthesis in insects and is vital for insect development and survival. Here, we clarify the physiological function of a CPR gene in Nilaparvata lugens, an important rice pest, by using RNA interference. CPR gene knockdown leads to the functional loss of waterproofing and water retention in the integument of female adults, which causes significantly reduced body weight and a lethal phenotype. Scanning electron microscopy shows that the lipid layer on the outermost surface of the abdominal cuticle becomes thin in dsCPR-injected adults. Furthermore, CHC profile analysis reveals that CPR knockdown significantly decreases the contents of CHCs with a carbon chain length ≥ C27 in adult females. Moreover, we find that CPR knockdown generates a deficient phenotype in ovaries with deformed oocytes and a complete failure of egg-laying. These findings suggest that CPR plays multiple functional roles in CHC biosynthesis and embryo development in insects. MDPI 2022-03-06 /pmc/articles/PMC8910901/ /pubmed/35270018 http://dx.doi.org/10.3390/ijms23052875 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Zhe-Chao
Tao, Shuai
Cheng, Xu
Li, Dan-Ting
Zhang, Chuan-Xi
Bao, Yan-Yuan
CPR Gene Contributes to Integument Function and Ovary Development in a Rice Planthopper
title CPR Gene Contributes to Integument Function and Ovary Development in a Rice Planthopper
title_full CPR Gene Contributes to Integument Function and Ovary Development in a Rice Planthopper
title_fullStr CPR Gene Contributes to Integument Function and Ovary Development in a Rice Planthopper
title_full_unstemmed CPR Gene Contributes to Integument Function and Ovary Development in a Rice Planthopper
title_short CPR Gene Contributes to Integument Function and Ovary Development in a Rice Planthopper
title_sort cpr gene contributes to integument function and ovary development in a rice planthopper
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8910901/
https://www.ncbi.nlm.nih.gov/pubmed/35270018
http://dx.doi.org/10.3390/ijms23052875
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