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Genome-Wide Investigation and Functional Verification of the ZIP Family Transporters in Wild Emmer Wheat

The zinc/iron-regulated transporter-like protein (ZIP) family has a crucial role in Zn homeostasis of plants. Although the ZIP genes have been systematically studied in many plant species, the significance of this family in wild emmer wheat (Triticum turgidum ssp. dicoccoides) is not yet well unders...

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Autores principales: Gong, Fangyi, Qi, Tiangang, Hu, Yanling, Jin, Yarong, Liu, Jia, Wang, Wenyang, He, Jingshu, Tu, Bin, Zhang, Tao, Jiang, Bo, Wang, Yi, Zhang, Lianquan, Zheng, Youliang, Liu, Dengcai, Huang, Lin, Wu, Bihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911026/
https://www.ncbi.nlm.nih.gov/pubmed/35270007
http://dx.doi.org/10.3390/ijms23052866
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author Gong, Fangyi
Qi, Tiangang
Hu, Yanling
Jin, Yarong
Liu, Jia
Wang, Wenyang
He, Jingshu
Tu, Bin
Zhang, Tao
Jiang, Bo
Wang, Yi
Zhang, Lianquan
Zheng, Youliang
Liu, Dengcai
Huang, Lin
Wu, Bihua
author_facet Gong, Fangyi
Qi, Tiangang
Hu, Yanling
Jin, Yarong
Liu, Jia
Wang, Wenyang
He, Jingshu
Tu, Bin
Zhang, Tao
Jiang, Bo
Wang, Yi
Zhang, Lianquan
Zheng, Youliang
Liu, Dengcai
Huang, Lin
Wu, Bihua
author_sort Gong, Fangyi
collection PubMed
description The zinc/iron-regulated transporter-like protein (ZIP) family has a crucial role in Zn homeostasis of plants. Although the ZIP genes have been systematically studied in many plant species, the significance of this family in wild emmer wheat (Triticum turgidum ssp. dicoccoides) is not yet well understood. In this study, a genome-wide investigation of ZIPs genes based on the wild emmer reference genome was conducted, and 33 TdZIP genes were identified. Protein structure analysis revealed that TdZIP proteins had 1 to 13 transmembrane (TM) domains and most of them were predicted to be located on the plasma membrane. These TdZIPs can be classified into three clades in a phylogenetic tree. They were annotated as being involved in inorganic ion transport and metabolism. Cis-acting analysis showed that several elements were involved in hormone, stresses, grain-filling, and plant development. Expression pattern analysis indicated that TdZIP genes were highly expressed in different tissues. TdZIP genes showed different expression patterns in response to Zn deficiency and that 11 genes were significantly induced in either roots or both roots and shoots of Zn-deficient plants. Yeast complementation analysis showed that TdZIP1A-3, TdZIP6B-1, TdZIP6B-2, TdZIP7A-3, and TdZIP7B-2 have the capacity to transport Zn. Overexpression of TdZIP6B-1 in rice showed increased Zn concentration in roots compared with wild-type plants. The expression levels of TdZIP6B-1 in transgenic rice were upregulated in normal Zn concentration compared to that of no Zn. This work provides a comprehensive understanding of the ZIP gene family in wild emmer wheat and paves the way for future functional analysis and genetic improvement of Zn deficiency tolerance in wheat.
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spelling pubmed-89110262022-03-11 Genome-Wide Investigation and Functional Verification of the ZIP Family Transporters in Wild Emmer Wheat Gong, Fangyi Qi, Tiangang Hu, Yanling Jin, Yarong Liu, Jia Wang, Wenyang He, Jingshu Tu, Bin Zhang, Tao Jiang, Bo Wang, Yi Zhang, Lianquan Zheng, Youliang Liu, Dengcai Huang, Lin Wu, Bihua Int J Mol Sci Article The zinc/iron-regulated transporter-like protein (ZIP) family has a crucial role in Zn homeostasis of plants. Although the ZIP genes have been systematically studied in many plant species, the significance of this family in wild emmer wheat (Triticum turgidum ssp. dicoccoides) is not yet well understood. In this study, a genome-wide investigation of ZIPs genes based on the wild emmer reference genome was conducted, and 33 TdZIP genes were identified. Protein structure analysis revealed that TdZIP proteins had 1 to 13 transmembrane (TM) domains and most of them were predicted to be located on the plasma membrane. These TdZIPs can be classified into three clades in a phylogenetic tree. They were annotated as being involved in inorganic ion transport and metabolism. Cis-acting analysis showed that several elements were involved in hormone, stresses, grain-filling, and plant development. Expression pattern analysis indicated that TdZIP genes were highly expressed in different tissues. TdZIP genes showed different expression patterns in response to Zn deficiency and that 11 genes were significantly induced in either roots or both roots and shoots of Zn-deficient plants. Yeast complementation analysis showed that TdZIP1A-3, TdZIP6B-1, TdZIP6B-2, TdZIP7A-3, and TdZIP7B-2 have the capacity to transport Zn. Overexpression of TdZIP6B-1 in rice showed increased Zn concentration in roots compared with wild-type plants. The expression levels of TdZIP6B-1 in transgenic rice were upregulated in normal Zn concentration compared to that of no Zn. This work provides a comprehensive understanding of the ZIP gene family in wild emmer wheat and paves the way for future functional analysis and genetic improvement of Zn deficiency tolerance in wheat. MDPI 2022-03-05 /pmc/articles/PMC8911026/ /pubmed/35270007 http://dx.doi.org/10.3390/ijms23052866 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gong, Fangyi
Qi, Tiangang
Hu, Yanling
Jin, Yarong
Liu, Jia
Wang, Wenyang
He, Jingshu
Tu, Bin
Zhang, Tao
Jiang, Bo
Wang, Yi
Zhang, Lianquan
Zheng, Youliang
Liu, Dengcai
Huang, Lin
Wu, Bihua
Genome-Wide Investigation and Functional Verification of the ZIP Family Transporters in Wild Emmer Wheat
title Genome-Wide Investigation and Functional Verification of the ZIP Family Transporters in Wild Emmer Wheat
title_full Genome-Wide Investigation and Functional Verification of the ZIP Family Transporters in Wild Emmer Wheat
title_fullStr Genome-Wide Investigation and Functional Verification of the ZIP Family Transporters in Wild Emmer Wheat
title_full_unstemmed Genome-Wide Investigation and Functional Verification of the ZIP Family Transporters in Wild Emmer Wheat
title_short Genome-Wide Investigation and Functional Verification of the ZIP Family Transporters in Wild Emmer Wheat
title_sort genome-wide investigation and functional verification of the zip family transporters in wild emmer wheat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911026/
https://www.ncbi.nlm.nih.gov/pubmed/35270007
http://dx.doi.org/10.3390/ijms23052866
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