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Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting
In patients with comorbidities, a large number of wounds become chronic, representing an overwhelming economic burden for healthcare systems. Engineering the microenvironment is a paramount trend to activate cells and burst-healing mechanisms. The extrusion bioprinting of advanced dressings was perf...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911091/ https://www.ncbi.nlm.nih.gov/pubmed/35269978 http://dx.doi.org/10.3390/ijms23052836 |
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author | Del Amo, Cristina Fernández-San Argimiro, Xabier Cascajo-Castresana, María Perez-Valle, Arantza Madarieta, Iratxe Olalde, Beatriz Andia, Isabel |
author_facet | Del Amo, Cristina Fernández-San Argimiro, Xabier Cascajo-Castresana, María Perez-Valle, Arantza Madarieta, Iratxe Olalde, Beatriz Andia, Isabel |
author_sort | Del Amo, Cristina |
collection | PubMed |
description | In patients with comorbidities, a large number of wounds become chronic, representing an overwhelming economic burden for healthcare systems. Engineering the microenvironment is a paramount trend to activate cells and burst-healing mechanisms. The extrusion bioprinting of advanced dressings was performed with novel composite bioinks made by blending adipose decellularized extracellular matrix with plasma and human dermal fibroblasts. Rheological and microstructural assessments of the composite hydrogels supported post-printing cell viability and proliferation over time. Embedded fibroblasts expressed steady concentrations of extracellular matrix proteins, including type 1, 3 and 4 collagens and fibronectin. ELISA assessments, multiplex protein arrays and ensuing bioinformatic analyses revealed paracrine activities corresponding to wound-healing activation through the modulation of inflammation and angiogenesis. The two modalities of advanced dressings, differing in platelet number, showed differences in the release of inflammatory and angiogenic cytokines, including interleukin 8 (IL-8), monocyte chemotactic protein 1 (MCP-1), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF). The conditioned media stimulated human-dermal-cell proliferation over time. Our findings open the door to engineering the microenvironment as a strategy to enhance healing. |
format | Online Article Text |
id | pubmed-8911091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89110912022-03-11 Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting Del Amo, Cristina Fernández-San Argimiro, Xabier Cascajo-Castresana, María Perez-Valle, Arantza Madarieta, Iratxe Olalde, Beatriz Andia, Isabel Int J Mol Sci Article In patients with comorbidities, a large number of wounds become chronic, representing an overwhelming economic burden for healthcare systems. Engineering the microenvironment is a paramount trend to activate cells and burst-healing mechanisms. The extrusion bioprinting of advanced dressings was performed with novel composite bioinks made by blending adipose decellularized extracellular matrix with plasma and human dermal fibroblasts. Rheological and microstructural assessments of the composite hydrogels supported post-printing cell viability and proliferation over time. Embedded fibroblasts expressed steady concentrations of extracellular matrix proteins, including type 1, 3 and 4 collagens and fibronectin. ELISA assessments, multiplex protein arrays and ensuing bioinformatic analyses revealed paracrine activities corresponding to wound-healing activation through the modulation of inflammation and angiogenesis. The two modalities of advanced dressings, differing in platelet number, showed differences in the release of inflammatory and angiogenic cytokines, including interleukin 8 (IL-8), monocyte chemotactic protein 1 (MCP-1), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF). The conditioned media stimulated human-dermal-cell proliferation over time. Our findings open the door to engineering the microenvironment as a strategy to enhance healing. MDPI 2022-03-04 /pmc/articles/PMC8911091/ /pubmed/35269978 http://dx.doi.org/10.3390/ijms23052836 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Del Amo, Cristina Fernández-San Argimiro, Xabier Cascajo-Castresana, María Perez-Valle, Arantza Madarieta, Iratxe Olalde, Beatriz Andia, Isabel Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting |
title | Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting |
title_full | Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting |
title_fullStr | Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting |
title_full_unstemmed | Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting |
title_short | Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting |
title_sort | wound-microenvironment engineering through advanced-dressing bioprinting |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911091/ https://www.ncbi.nlm.nih.gov/pubmed/35269978 http://dx.doi.org/10.3390/ijms23052836 |
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