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Identification of the Regulators of Epidermis Development under Drought- and Salt-Stressed Conditions by Single-Cell RNA-Seq

As sessile organisms, plants constantly face challenges from the external environment. In order to meet these challenges and survive, plants have evolved a set of sophisticated adaptation strategies, including changes in leaf morphology and epidermal cell development. These developmental patterns ar...

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Autores principales: Liu, Zhixin, Guo, Chenxi, Wu, Rui, Wang, Jiajing, Zhou, Yaping, Yu, Xiaole, Zhang, Yixin, Zhao, Zihao, Liu, Hao, Sun, Susu, Hu, Mengke, Qin, Aizhi, Liu, Yumeng, Yang, Jincheng, Bawa, George, Sun, Xuwu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911155/
https://www.ncbi.nlm.nih.gov/pubmed/35269904
http://dx.doi.org/10.3390/ijms23052759
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author Liu, Zhixin
Guo, Chenxi
Wu, Rui
Wang, Jiajing
Zhou, Yaping
Yu, Xiaole
Zhang, Yixin
Zhao, Zihao
Liu, Hao
Sun, Susu
Hu, Mengke
Qin, Aizhi
Liu, Yumeng
Yang, Jincheng
Bawa, George
Sun, Xuwu
author_facet Liu, Zhixin
Guo, Chenxi
Wu, Rui
Wang, Jiajing
Zhou, Yaping
Yu, Xiaole
Zhang, Yixin
Zhao, Zihao
Liu, Hao
Sun, Susu
Hu, Mengke
Qin, Aizhi
Liu, Yumeng
Yang, Jincheng
Bawa, George
Sun, Xuwu
author_sort Liu, Zhixin
collection PubMed
description As sessile organisms, plants constantly face challenges from the external environment. In order to meet these challenges and survive, plants have evolved a set of sophisticated adaptation strategies, including changes in leaf morphology and epidermal cell development. These developmental patterns are regulated by both light and hormonal signaling pathways. However, our mechanistic understanding of the role of these signaling pathways in regulating plant response to environmental stress is still very limited. By applying single-cell RNA-Seq, we determined the expression pattern of PHYTOCHROME INTERACTING FACTOR (PIF) 1, PIF3, PIF4, and PIF5 genes in leaf epidermal pavement cells (PCs) and guard cells (GCs). PCs and GCs are very sensitive to environmental stress, and our previous research suggests that these PIFs may be involved in regulating the development of PCs, GCs, and leaf morphology under environmental stress. Growth analysis showed that pif1/3/4/5 quadruple mutant maintained tolerance to drought and salt stress, and the length to width ratio of leaves and petiole length under normal growth conditions were similar to those of wild-type (WT) plants under drought and salt treatment. Analysis of the developmental patterns of PCs and GCs, and whole leaf morphology, further confirmed that these PIFs may be involved in mediating the development of epidermal cells under drought and salt stress, likely by regulating the expression of MUTE and TOO MANY MOUTHS (TMM) genes. These results provide new insights into the molecular mechanism of plant adaptation to adverse growth environments.
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spelling pubmed-89111552022-03-11 Identification of the Regulators of Epidermis Development under Drought- and Salt-Stressed Conditions by Single-Cell RNA-Seq Liu, Zhixin Guo, Chenxi Wu, Rui Wang, Jiajing Zhou, Yaping Yu, Xiaole Zhang, Yixin Zhao, Zihao Liu, Hao Sun, Susu Hu, Mengke Qin, Aizhi Liu, Yumeng Yang, Jincheng Bawa, George Sun, Xuwu Int J Mol Sci Article As sessile organisms, plants constantly face challenges from the external environment. In order to meet these challenges and survive, plants have evolved a set of sophisticated adaptation strategies, including changes in leaf morphology and epidermal cell development. These developmental patterns are regulated by both light and hormonal signaling pathways. However, our mechanistic understanding of the role of these signaling pathways in regulating plant response to environmental stress is still very limited. By applying single-cell RNA-Seq, we determined the expression pattern of PHYTOCHROME INTERACTING FACTOR (PIF) 1, PIF3, PIF4, and PIF5 genes in leaf epidermal pavement cells (PCs) and guard cells (GCs). PCs and GCs are very sensitive to environmental stress, and our previous research suggests that these PIFs may be involved in regulating the development of PCs, GCs, and leaf morphology under environmental stress. Growth analysis showed that pif1/3/4/5 quadruple mutant maintained tolerance to drought and salt stress, and the length to width ratio of leaves and petiole length under normal growth conditions were similar to those of wild-type (WT) plants under drought and salt treatment. Analysis of the developmental patterns of PCs and GCs, and whole leaf morphology, further confirmed that these PIFs may be involved in mediating the development of epidermal cells under drought and salt stress, likely by regulating the expression of MUTE and TOO MANY MOUTHS (TMM) genes. These results provide new insights into the molecular mechanism of plant adaptation to adverse growth environments. MDPI 2022-03-02 /pmc/articles/PMC8911155/ /pubmed/35269904 http://dx.doi.org/10.3390/ijms23052759 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Zhixin
Guo, Chenxi
Wu, Rui
Wang, Jiajing
Zhou, Yaping
Yu, Xiaole
Zhang, Yixin
Zhao, Zihao
Liu, Hao
Sun, Susu
Hu, Mengke
Qin, Aizhi
Liu, Yumeng
Yang, Jincheng
Bawa, George
Sun, Xuwu
Identification of the Regulators of Epidermis Development under Drought- and Salt-Stressed Conditions by Single-Cell RNA-Seq
title Identification of the Regulators of Epidermis Development under Drought- and Salt-Stressed Conditions by Single-Cell RNA-Seq
title_full Identification of the Regulators of Epidermis Development under Drought- and Salt-Stressed Conditions by Single-Cell RNA-Seq
title_fullStr Identification of the Regulators of Epidermis Development under Drought- and Salt-Stressed Conditions by Single-Cell RNA-Seq
title_full_unstemmed Identification of the Regulators of Epidermis Development under Drought- and Salt-Stressed Conditions by Single-Cell RNA-Seq
title_short Identification of the Regulators of Epidermis Development under Drought- and Salt-Stressed Conditions by Single-Cell RNA-Seq
title_sort identification of the regulators of epidermis development under drought- and salt-stressed conditions by single-cell rna-seq
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911155/
https://www.ncbi.nlm.nih.gov/pubmed/35269904
http://dx.doi.org/10.3390/ijms23052759
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