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Antioxidative and Anti-Inflammatory Activities of Chrysin and Naringenin in a Drug-Induced Bone Loss Model in Rats

Oxidative stress (OS) mediators, together with the inflammatory processes, are considered as threatening factors for bone health. The aim of this study was to investigate effects of flavonoids naringenin and chrysin on OS, inflammation, and bone degradation in retinoic acid (13cRA)-induced secondary...

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Autores principales: Oršolić, Nada, Nemrava, Johann, Jeleč, Željko, Kukolj, Marina, Odeh, Dyana, Jakopović, Boris, Jazvinšćak Jembrek, Maja, Bagatin, Tomica, Fureš, Rajko, Bagatin, Dinko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911302/
https://www.ncbi.nlm.nih.gov/pubmed/35270014
http://dx.doi.org/10.3390/ijms23052872
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author Oršolić, Nada
Nemrava, Johann
Jeleč, Željko
Kukolj, Marina
Odeh, Dyana
Jakopović, Boris
Jazvinšćak Jembrek, Maja
Bagatin, Tomica
Fureš, Rajko
Bagatin, Dinko
author_facet Oršolić, Nada
Nemrava, Johann
Jeleč, Željko
Kukolj, Marina
Odeh, Dyana
Jakopović, Boris
Jazvinšćak Jembrek, Maja
Bagatin, Tomica
Fureš, Rajko
Bagatin, Dinko
author_sort Oršolić, Nada
collection PubMed
description Oxidative stress (OS) mediators, together with the inflammatory processes, are considered as threatening factors for bone health. The aim of this study was to investigate effects of flavonoids naringenin and chrysin on OS, inflammation, and bone degradation in retinoic acid (13cRA)-induced secondary osteoporosis (OP) in rats. We analysed changes in body and uterine weight, biochemical bone parameters (bone mineral density (BMD), bone mineral content (BMC), markers of bone turnover), bone geometry parameters, bone histology, OS parameters, biochemical and haematological parameters, and levels of inflammatory cytokines. Osteoporotic rats had reduced bone Ca and P levels, BMD, BMC, and expression of markers of bone turnover, and increased values of serum enzymes alkaline phosphatase (ALP) and lactate dehydrogenase (LDH). Malondialdehyde (MDA) production in liver, kidney, and ovary was increased, while the glutathione (GSH) content and activities of antioxidant enzymes were reduced and accompanied with the enhanced release of inflammatory mediators TNF-α, IL-1β, IL-6, and RANTES chemokine (regulated on activation normal T cell expressed and secreted) in serum. Treatment with chrysin or naringenin improved bone quality, reduced bone resorption, and bone mineral deposition, although with a lower efficacy compared with alendronate. However, flavonoids exhibited more pronounced antioxidative, anti-inflammatory and phytoestrogenic activities, indicating their great potential in attenuating bone loss and prevention of OP.
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spelling pubmed-89113022022-03-11 Antioxidative and Anti-Inflammatory Activities of Chrysin and Naringenin in a Drug-Induced Bone Loss Model in Rats Oršolić, Nada Nemrava, Johann Jeleč, Željko Kukolj, Marina Odeh, Dyana Jakopović, Boris Jazvinšćak Jembrek, Maja Bagatin, Tomica Fureš, Rajko Bagatin, Dinko Int J Mol Sci Article Oxidative stress (OS) mediators, together with the inflammatory processes, are considered as threatening factors for bone health. The aim of this study was to investigate effects of flavonoids naringenin and chrysin on OS, inflammation, and bone degradation in retinoic acid (13cRA)-induced secondary osteoporosis (OP) in rats. We analysed changes in body and uterine weight, biochemical bone parameters (bone mineral density (BMD), bone mineral content (BMC), markers of bone turnover), bone geometry parameters, bone histology, OS parameters, biochemical and haematological parameters, and levels of inflammatory cytokines. Osteoporotic rats had reduced bone Ca and P levels, BMD, BMC, and expression of markers of bone turnover, and increased values of serum enzymes alkaline phosphatase (ALP) and lactate dehydrogenase (LDH). Malondialdehyde (MDA) production in liver, kidney, and ovary was increased, while the glutathione (GSH) content and activities of antioxidant enzymes were reduced and accompanied with the enhanced release of inflammatory mediators TNF-α, IL-1β, IL-6, and RANTES chemokine (regulated on activation normal T cell expressed and secreted) in serum. Treatment with chrysin or naringenin improved bone quality, reduced bone resorption, and bone mineral deposition, although with a lower efficacy compared with alendronate. However, flavonoids exhibited more pronounced antioxidative, anti-inflammatory and phytoestrogenic activities, indicating their great potential in attenuating bone loss and prevention of OP. MDPI 2022-03-06 /pmc/articles/PMC8911302/ /pubmed/35270014 http://dx.doi.org/10.3390/ijms23052872 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oršolić, Nada
Nemrava, Johann
Jeleč, Željko
Kukolj, Marina
Odeh, Dyana
Jakopović, Boris
Jazvinšćak Jembrek, Maja
Bagatin, Tomica
Fureš, Rajko
Bagatin, Dinko
Antioxidative and Anti-Inflammatory Activities of Chrysin and Naringenin in a Drug-Induced Bone Loss Model in Rats
title Antioxidative and Anti-Inflammatory Activities of Chrysin and Naringenin in a Drug-Induced Bone Loss Model in Rats
title_full Antioxidative and Anti-Inflammatory Activities of Chrysin and Naringenin in a Drug-Induced Bone Loss Model in Rats
title_fullStr Antioxidative and Anti-Inflammatory Activities of Chrysin and Naringenin in a Drug-Induced Bone Loss Model in Rats
title_full_unstemmed Antioxidative and Anti-Inflammatory Activities of Chrysin and Naringenin in a Drug-Induced Bone Loss Model in Rats
title_short Antioxidative and Anti-Inflammatory Activities of Chrysin and Naringenin in a Drug-Induced Bone Loss Model in Rats
title_sort antioxidative and anti-inflammatory activities of chrysin and naringenin in a drug-induced bone loss model in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911302/
https://www.ncbi.nlm.nih.gov/pubmed/35270014
http://dx.doi.org/10.3390/ijms23052872
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