Application of β-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol

Resveratrol, an ingredient of traditional Chinese medicine, has beneficial effects on human health and huge potential for application in modern medicine. Polydatin is extracted from plants and then deglycosylated into resveratrol; enzymatic methods are preferred for this reaction. In this study, a β...

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Autores principales: Zhou, Jie, Liang, Meng, Lin, Yu, Pang, Hao, Wei, Yutuo, Huang, Ribo, Du, Liqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911618/
https://www.ncbi.nlm.nih.gov/pubmed/35268615
http://dx.doi.org/10.3390/molecules27051514
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author Zhou, Jie
Liang, Meng
Lin, Yu
Pang, Hao
Wei, Yutuo
Huang, Ribo
Du, Liqin
author_facet Zhou, Jie
Liang, Meng
Lin, Yu
Pang, Hao
Wei, Yutuo
Huang, Ribo
Du, Liqin
author_sort Zhou, Jie
collection PubMed
description Resveratrol, an ingredient of traditional Chinese medicine, has beneficial effects on human health and huge potential for application in modern medicine. Polydatin is extracted from plants and then deglycosylated into resveratrol; enzymatic methods are preferred for this reaction. In this study, a β-D-glucosidase from Sphingomonas showed high efficiency in transforming polydatin into resveratrol and was tolerant toward organic solvents. Applying this enzyme in a biphasic transformation system resulted in 95.3% conversion of 20% concentration crude polydatin to resveratrol in 4 h. We thus report a new method for high-efficiency, clean production of resveratrol.
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spelling pubmed-89116182022-03-11 Application of β-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol Zhou, Jie Liang, Meng Lin, Yu Pang, Hao Wei, Yutuo Huang, Ribo Du, Liqin Molecules Article Resveratrol, an ingredient of traditional Chinese medicine, has beneficial effects on human health and huge potential for application in modern medicine. Polydatin is extracted from plants and then deglycosylated into resveratrol; enzymatic methods are preferred for this reaction. In this study, a β-D-glucosidase from Sphingomonas showed high efficiency in transforming polydatin into resveratrol and was tolerant toward organic solvents. Applying this enzyme in a biphasic transformation system resulted in 95.3% conversion of 20% concentration crude polydatin to resveratrol in 4 h. We thus report a new method for high-efficiency, clean production of resveratrol. MDPI 2022-02-23 /pmc/articles/PMC8911618/ /pubmed/35268615 http://dx.doi.org/10.3390/molecules27051514 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Jie
Liang, Meng
Lin, Yu
Pang, Hao
Wei, Yutuo
Huang, Ribo
Du, Liqin
Application of β-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol
title Application of β-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol
title_full Application of β-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol
title_fullStr Application of β-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol
title_full_unstemmed Application of β-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol
title_short Application of β-Glucosidase in a Biphasic System for the Efficient Conversion of Polydatin to Resveratrol
title_sort application of β-glucosidase in a biphasic system for the efficient conversion of polydatin to resveratrol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911618/
https://www.ncbi.nlm.nih.gov/pubmed/35268615
http://dx.doi.org/10.3390/molecules27051514
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