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Decellularized Avian Cartilage, a Promising Alternative for Human Cartilage Tissue Regeneration

Articular cartilage defects, and subsequent degeneration, are prevalent and account for the poor quality of life of most elderly persons; they are also one of the main predisposing factors to osteoarthritis. Articular cartilage is an avascular tissue and, thus, has limited capacity for healing and s...

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Autores principales: Ayariga, Joseph Atia, Huang, Hanxiao, Dean, Derrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911734/
https://www.ncbi.nlm.nih.gov/pubmed/35269204
http://dx.doi.org/10.3390/ma15051974
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author Ayariga, Joseph Atia
Huang, Hanxiao
Dean, Derrick
author_facet Ayariga, Joseph Atia
Huang, Hanxiao
Dean, Derrick
author_sort Ayariga, Joseph Atia
collection PubMed
description Articular cartilage defects, and subsequent degeneration, are prevalent and account for the poor quality of life of most elderly persons; they are also one of the main predisposing factors to osteoarthritis. Articular cartilage is an avascular tissue and, thus, has limited capacity for healing and self-repair. Damage to the articular cartilage by trauma or pathological causes is irreversible. Many approaches to repair cartilage have been attempted with some potential; however, there is no consensus on any ideal therapy. Tissue engineering holds promise as an approach to regenerate damaged cartilage. Since cell adhesion is a critical step in tissue engineering, providing a 3D microenvironment that recapitulates the cartilage tissue is vital to inducing cartilage regeneration. Decellularized materials have emerged as promising scaffolds for tissue engineering, since this procedure produces scaffolds from native tissues that possess structural and chemical natures that are mimetic of the extracellular matrix (ECM) of the native tissue. In this work, we present, for the first time, a study of decellularized scaffolds, produced from avian articular cartilage (extracted from Gallus Gallus domesticus), reseeded with human chondrocytes, and we demonstrate for the first time that human chondrocytes survived, proliferated and interacted with the scaffolds. Morphological studies of the decellularized scaffolds revealed an interconnected, porous architecture, ideal for cell growth. Mechanical characterization showed that the decellularized scaffolds registered stiffness comparable to the native cartilage tissues. Cell growth inhibition and immunocytochemical analyses showed that the decellularized scaffolds are suitable for cartilage regeneration.
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spelling pubmed-89117342022-03-11 Decellularized Avian Cartilage, a Promising Alternative for Human Cartilage Tissue Regeneration Ayariga, Joseph Atia Huang, Hanxiao Dean, Derrick Materials (Basel) Article Articular cartilage defects, and subsequent degeneration, are prevalent and account for the poor quality of life of most elderly persons; they are also one of the main predisposing factors to osteoarthritis. Articular cartilage is an avascular tissue and, thus, has limited capacity for healing and self-repair. Damage to the articular cartilage by trauma or pathological causes is irreversible. Many approaches to repair cartilage have been attempted with some potential; however, there is no consensus on any ideal therapy. Tissue engineering holds promise as an approach to regenerate damaged cartilage. Since cell adhesion is a critical step in tissue engineering, providing a 3D microenvironment that recapitulates the cartilage tissue is vital to inducing cartilage regeneration. Decellularized materials have emerged as promising scaffolds for tissue engineering, since this procedure produces scaffolds from native tissues that possess structural and chemical natures that are mimetic of the extracellular matrix (ECM) of the native tissue. In this work, we present, for the first time, a study of decellularized scaffolds, produced from avian articular cartilage (extracted from Gallus Gallus domesticus), reseeded with human chondrocytes, and we demonstrate for the first time that human chondrocytes survived, proliferated and interacted with the scaffolds. Morphological studies of the decellularized scaffolds revealed an interconnected, porous architecture, ideal for cell growth. Mechanical characterization showed that the decellularized scaffolds registered stiffness comparable to the native cartilage tissues. Cell growth inhibition and immunocytochemical analyses showed that the decellularized scaffolds are suitable for cartilage regeneration. MDPI 2022-03-07 /pmc/articles/PMC8911734/ /pubmed/35269204 http://dx.doi.org/10.3390/ma15051974 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ayariga, Joseph Atia
Huang, Hanxiao
Dean, Derrick
Decellularized Avian Cartilage, a Promising Alternative for Human Cartilage Tissue Regeneration
title Decellularized Avian Cartilage, a Promising Alternative for Human Cartilage Tissue Regeneration
title_full Decellularized Avian Cartilage, a Promising Alternative for Human Cartilage Tissue Regeneration
title_fullStr Decellularized Avian Cartilage, a Promising Alternative for Human Cartilage Tissue Regeneration
title_full_unstemmed Decellularized Avian Cartilage, a Promising Alternative for Human Cartilage Tissue Regeneration
title_short Decellularized Avian Cartilage, a Promising Alternative for Human Cartilage Tissue Regeneration
title_sort decellularized avian cartilage, a promising alternative for human cartilage tissue regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911734/
https://www.ncbi.nlm.nih.gov/pubmed/35269204
http://dx.doi.org/10.3390/ma15051974
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