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Construction of Orthogonal Modular Proteinaceous Nanovaccine Delivery Vectors Based on mSA-Biotin Binding

Proteinaceous nanovaccine delivery systems have significantly promoted the development of various high-efficiency vaccines. However, the widely used method of coupling the expression of scaffolds and antigens may result in their structural interference with each other. Monovalent streptavidin (mSA)...

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Autores principales: Shi, Yixin, Pan, Chao, Wang, Kangfeng, Liu, Yan, Sun, Yange, Guo, Yan, Sun, Peng, Wu, Jun, Lu, Ying, Zhu, Li, Wang, Hengliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911943/
https://www.ncbi.nlm.nih.gov/pubmed/35269221
http://dx.doi.org/10.3390/nano12050734
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author Shi, Yixin
Pan, Chao
Wang, Kangfeng
Liu, Yan
Sun, Yange
Guo, Yan
Sun, Peng
Wu, Jun
Lu, Ying
Zhu, Li
Wang, Hengliang
author_facet Shi, Yixin
Pan, Chao
Wang, Kangfeng
Liu, Yan
Sun, Yange
Guo, Yan
Sun, Peng
Wu, Jun
Lu, Ying
Zhu, Li
Wang, Hengliang
author_sort Shi, Yixin
collection PubMed
description Proteinaceous nanovaccine delivery systems have significantly promoted the development of various high-efficiency vaccines. However, the widely used method of coupling the expression of scaffolds and antigens may result in their structural interference with each other. Monovalent streptavidin (mSA) is a short monomer sequence, which has a strong affinity for biotin. Here, we discuss an orthogonal, modular, and highly versatile self-assembled proteinaceous nanoparticle chassis that facilitates combinations with various antigen cargos by using mSA and biotin to produce nanovaccines. We first improved the yield of these nanoparticles by appending a short sugar chain on their surfaces in a constructed host strain. After confirming the strong ability to induce both Th1- and Th2-mediated immune responses based on the plasma cytokine spectrum from immunized mice, we further verified the binding ability of biotinylated nanoparticles to mSA-antigens. These results demonstrate that our biotinylated nanoparticle chassis could load both protein and polysaccharide antigens containing mSA at a high affinity. Our approach thus offers an attractive technology for combining nanoparticles and antigen cargos to generate various high-performance nanovaccines. In particular, the designed mSA connector (mSA containing glycosylation modification sequences) could couple with polysaccharide antigens, providing a new attractive strategy to prepare nanoscale conjugate vaccines.
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spelling pubmed-89119432022-03-11 Construction of Orthogonal Modular Proteinaceous Nanovaccine Delivery Vectors Based on mSA-Biotin Binding Shi, Yixin Pan, Chao Wang, Kangfeng Liu, Yan Sun, Yange Guo, Yan Sun, Peng Wu, Jun Lu, Ying Zhu, Li Wang, Hengliang Nanomaterials (Basel) Article Proteinaceous nanovaccine delivery systems have significantly promoted the development of various high-efficiency vaccines. However, the widely used method of coupling the expression of scaffolds and antigens may result in their structural interference with each other. Monovalent streptavidin (mSA) is a short monomer sequence, which has a strong affinity for biotin. Here, we discuss an orthogonal, modular, and highly versatile self-assembled proteinaceous nanoparticle chassis that facilitates combinations with various antigen cargos by using mSA and biotin to produce nanovaccines. We first improved the yield of these nanoparticles by appending a short sugar chain on their surfaces in a constructed host strain. After confirming the strong ability to induce both Th1- and Th2-mediated immune responses based on the plasma cytokine spectrum from immunized mice, we further verified the binding ability of biotinylated nanoparticles to mSA-antigens. These results demonstrate that our biotinylated nanoparticle chassis could load both protein and polysaccharide antigens containing mSA at a high affinity. Our approach thus offers an attractive technology for combining nanoparticles and antigen cargos to generate various high-performance nanovaccines. In particular, the designed mSA connector (mSA containing glycosylation modification sequences) could couple with polysaccharide antigens, providing a new attractive strategy to prepare nanoscale conjugate vaccines. MDPI 2022-02-22 /pmc/articles/PMC8911943/ /pubmed/35269221 http://dx.doi.org/10.3390/nano12050734 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shi, Yixin
Pan, Chao
Wang, Kangfeng
Liu, Yan
Sun, Yange
Guo, Yan
Sun, Peng
Wu, Jun
Lu, Ying
Zhu, Li
Wang, Hengliang
Construction of Orthogonal Modular Proteinaceous Nanovaccine Delivery Vectors Based on mSA-Biotin Binding
title Construction of Orthogonal Modular Proteinaceous Nanovaccine Delivery Vectors Based on mSA-Biotin Binding
title_full Construction of Orthogonal Modular Proteinaceous Nanovaccine Delivery Vectors Based on mSA-Biotin Binding
title_fullStr Construction of Orthogonal Modular Proteinaceous Nanovaccine Delivery Vectors Based on mSA-Biotin Binding
title_full_unstemmed Construction of Orthogonal Modular Proteinaceous Nanovaccine Delivery Vectors Based on mSA-Biotin Binding
title_short Construction of Orthogonal Modular Proteinaceous Nanovaccine Delivery Vectors Based on mSA-Biotin Binding
title_sort construction of orthogonal modular proteinaceous nanovaccine delivery vectors based on msa-biotin binding
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8911943/
https://www.ncbi.nlm.nih.gov/pubmed/35269221
http://dx.doi.org/10.3390/nano12050734
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