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New Red-Shifted 4-Styrylcoumarin Derivatives as Potential Fluorescent Labels for Biomolecules
Important scientific areas, such as cellular biology, medicine, pharmacy, and environmental sciences, are dependent on very sensitive analytical techniques to track and detect biomolecules. In this work, we develop a simple, low-cost and effective synthetic strategy to produce new red-shifted 4-styr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912076/ https://www.ncbi.nlm.nih.gov/pubmed/35268562 http://dx.doi.org/10.3390/molecules27051461 |
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author | Eustáquio, Raquel Ramalho, João P. Prates Caldeira, Ana T. Pereira, António |
author_facet | Eustáquio, Raquel Ramalho, João P. Prates Caldeira, Ana T. Pereira, António |
author_sort | Eustáquio, Raquel |
collection | PubMed |
description | Important scientific areas, such as cellular biology, medicine, pharmacy, and environmental sciences, are dependent on very sensitive analytical techniques to track and detect biomolecules. In this work, we develop a simple, low-cost and effective synthetic strategy to produce new red-shifted 4-styrylcoumarin derivatives as promising inexpensive fluorescent labels for biomolecules. The extension of the delocalized π-electron system results in bathochromic shifts in these new coumarin derivatives, which also present large Stokes shifts. In addition, density functional theory and time-dependent density functional theory calculations helped to rationalize the photophysical properties observed by the experimental results. |
format | Online Article Text |
id | pubmed-8912076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89120762022-03-11 New Red-Shifted 4-Styrylcoumarin Derivatives as Potential Fluorescent Labels for Biomolecules Eustáquio, Raquel Ramalho, João P. Prates Caldeira, Ana T. Pereira, António Molecules Article Important scientific areas, such as cellular biology, medicine, pharmacy, and environmental sciences, are dependent on very sensitive analytical techniques to track and detect biomolecules. In this work, we develop a simple, low-cost and effective synthetic strategy to produce new red-shifted 4-styrylcoumarin derivatives as promising inexpensive fluorescent labels for biomolecules. The extension of the delocalized π-electron system results in bathochromic shifts in these new coumarin derivatives, which also present large Stokes shifts. In addition, density functional theory and time-dependent density functional theory calculations helped to rationalize the photophysical properties observed by the experimental results. MDPI 2022-02-22 /pmc/articles/PMC8912076/ /pubmed/35268562 http://dx.doi.org/10.3390/molecules27051461 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Eustáquio, Raquel Ramalho, João P. Prates Caldeira, Ana T. Pereira, António New Red-Shifted 4-Styrylcoumarin Derivatives as Potential Fluorescent Labels for Biomolecules |
title | New Red-Shifted 4-Styrylcoumarin Derivatives as Potential Fluorescent Labels for Biomolecules |
title_full | New Red-Shifted 4-Styrylcoumarin Derivatives as Potential Fluorescent Labels for Biomolecules |
title_fullStr | New Red-Shifted 4-Styrylcoumarin Derivatives as Potential Fluorescent Labels for Biomolecules |
title_full_unstemmed | New Red-Shifted 4-Styrylcoumarin Derivatives as Potential Fluorescent Labels for Biomolecules |
title_short | New Red-Shifted 4-Styrylcoumarin Derivatives as Potential Fluorescent Labels for Biomolecules |
title_sort | new red-shifted 4-styrylcoumarin derivatives as potential fluorescent labels for biomolecules |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912076/ https://www.ncbi.nlm.nih.gov/pubmed/35268562 http://dx.doi.org/10.3390/molecules27051461 |
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