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The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity

A large number of aerospace practices have confirmed that the aerospace microgravity environment can lead to cognitive function decline. Mitochondria are the most important energy metabolism organelles, and some studies demonstrate that the areospace microgravity environment can cause mitochondrial...

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Autores principales: Ji, Guohua, Chang, Hui, Yang, Mingsi, Chen, Hailong, Wang, Tingmei, Liu, Xu, Lv, Ke, Li, Yinghui, Song, Bo, Qu, Lina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912132/
https://www.ncbi.nlm.nih.gov/pubmed/35271667
http://dx.doi.org/10.1371/journal.pone.0265108
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author Ji, Guohua
Chang, Hui
Yang, Mingsi
Chen, Hailong
Wang, Tingmei
Liu, Xu
Lv, Ke
Li, Yinghui
Song, Bo
Qu, Lina
author_facet Ji, Guohua
Chang, Hui
Yang, Mingsi
Chen, Hailong
Wang, Tingmei
Liu, Xu
Lv, Ke
Li, Yinghui
Song, Bo
Qu, Lina
author_sort Ji, Guohua
collection PubMed
description A large number of aerospace practices have confirmed that the aerospace microgravity environment can lead to cognitive function decline. Mitochondria are the most important energy metabolism organelles, and some studies demonstrate that the areospace microgravity environment can cause mitochondrial dysfunction. However, the relationships between cognitive function decline and mitochondrial dysfunction in the microgravity environment have not been elucidated. In this study, we simulated the microgravity environment in the Sprague-Dawley (SD) rats by -30° tail suspension for 28 days. We then investigated the changes of mitochondrial morphology and proteomics in the hippocampus. The electron microscopy results showed that the 28-day tail suspension increased the mitochondria number and size of rat hippocampal neuronal soma. Using TMT-based proteomics analysis, we identified 163 differentially expressed proteins (DEPs) between tail suspension and control samples, and among them, 128 proteins were upregulated and 35 proteins were downregulated. Functional and network analyses of the DEPs indicated that several of mitochondrial metabolic processes including the tricarboxylic acid (TCA) cycle were altered by simulating microgravity (SM). We verified 3 upregulated proteins, aconitate hydratase (ACO2), dihydrolipoamide S-succinyltransferase (DLST), and citrate synthase (CS), in the TCA cycle process by western blotting and confirmed their differential expressions between tail suspension and control samples. Taken together, our results demonstrate that 28-day tail suspension can cause changes in the morphology and metabolic function of hippocampus mitochondria, which might represent a mechanism of cognitive disorder caused by aerospace microgravity.
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spelling pubmed-89121322022-03-11 The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity Ji, Guohua Chang, Hui Yang, Mingsi Chen, Hailong Wang, Tingmei Liu, Xu Lv, Ke Li, Yinghui Song, Bo Qu, Lina PLoS One Research Article A large number of aerospace practices have confirmed that the aerospace microgravity environment can lead to cognitive function decline. Mitochondria are the most important energy metabolism organelles, and some studies demonstrate that the areospace microgravity environment can cause mitochondrial dysfunction. However, the relationships between cognitive function decline and mitochondrial dysfunction in the microgravity environment have not been elucidated. In this study, we simulated the microgravity environment in the Sprague-Dawley (SD) rats by -30° tail suspension for 28 days. We then investigated the changes of mitochondrial morphology and proteomics in the hippocampus. The electron microscopy results showed that the 28-day tail suspension increased the mitochondria number and size of rat hippocampal neuronal soma. Using TMT-based proteomics analysis, we identified 163 differentially expressed proteins (DEPs) between tail suspension and control samples, and among them, 128 proteins were upregulated and 35 proteins were downregulated. Functional and network analyses of the DEPs indicated that several of mitochondrial metabolic processes including the tricarboxylic acid (TCA) cycle were altered by simulating microgravity (SM). We verified 3 upregulated proteins, aconitate hydratase (ACO2), dihydrolipoamide S-succinyltransferase (DLST), and citrate synthase (CS), in the TCA cycle process by western blotting and confirmed their differential expressions between tail suspension and control samples. Taken together, our results demonstrate that 28-day tail suspension can cause changes in the morphology and metabolic function of hippocampus mitochondria, which might represent a mechanism of cognitive disorder caused by aerospace microgravity. Public Library of Science 2022-03-10 /pmc/articles/PMC8912132/ /pubmed/35271667 http://dx.doi.org/10.1371/journal.pone.0265108 Text en © 2022 Ji et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ji, Guohua
Chang, Hui
Yang, Mingsi
Chen, Hailong
Wang, Tingmei
Liu, Xu
Lv, Ke
Li, Yinghui
Song, Bo
Qu, Lina
The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity
title The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity
title_full The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity
title_fullStr The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity
title_full_unstemmed The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity
title_short The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity
title_sort mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912132/
https://www.ncbi.nlm.nih.gov/pubmed/35271667
http://dx.doi.org/10.1371/journal.pone.0265108
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