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The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity
A large number of aerospace practices have confirmed that the aerospace microgravity environment can lead to cognitive function decline. Mitochondria are the most important energy metabolism organelles, and some studies demonstrate that the areospace microgravity environment can cause mitochondrial...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912132/ https://www.ncbi.nlm.nih.gov/pubmed/35271667 http://dx.doi.org/10.1371/journal.pone.0265108 |
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author | Ji, Guohua Chang, Hui Yang, Mingsi Chen, Hailong Wang, Tingmei Liu, Xu Lv, Ke Li, Yinghui Song, Bo Qu, Lina |
author_facet | Ji, Guohua Chang, Hui Yang, Mingsi Chen, Hailong Wang, Tingmei Liu, Xu Lv, Ke Li, Yinghui Song, Bo Qu, Lina |
author_sort | Ji, Guohua |
collection | PubMed |
description | A large number of aerospace practices have confirmed that the aerospace microgravity environment can lead to cognitive function decline. Mitochondria are the most important energy metabolism organelles, and some studies demonstrate that the areospace microgravity environment can cause mitochondrial dysfunction. However, the relationships between cognitive function decline and mitochondrial dysfunction in the microgravity environment have not been elucidated. In this study, we simulated the microgravity environment in the Sprague-Dawley (SD) rats by -30° tail suspension for 28 days. We then investigated the changes of mitochondrial morphology and proteomics in the hippocampus. The electron microscopy results showed that the 28-day tail suspension increased the mitochondria number and size of rat hippocampal neuronal soma. Using TMT-based proteomics analysis, we identified 163 differentially expressed proteins (DEPs) between tail suspension and control samples, and among them, 128 proteins were upregulated and 35 proteins were downregulated. Functional and network analyses of the DEPs indicated that several of mitochondrial metabolic processes including the tricarboxylic acid (TCA) cycle were altered by simulating microgravity (SM). We verified 3 upregulated proteins, aconitate hydratase (ACO2), dihydrolipoamide S-succinyltransferase (DLST), and citrate synthase (CS), in the TCA cycle process by western blotting and confirmed their differential expressions between tail suspension and control samples. Taken together, our results demonstrate that 28-day tail suspension can cause changes in the morphology and metabolic function of hippocampus mitochondria, which might represent a mechanism of cognitive disorder caused by aerospace microgravity. |
format | Online Article Text |
id | pubmed-8912132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-89121322022-03-11 The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity Ji, Guohua Chang, Hui Yang, Mingsi Chen, Hailong Wang, Tingmei Liu, Xu Lv, Ke Li, Yinghui Song, Bo Qu, Lina PLoS One Research Article A large number of aerospace practices have confirmed that the aerospace microgravity environment can lead to cognitive function decline. Mitochondria are the most important energy metabolism organelles, and some studies demonstrate that the areospace microgravity environment can cause mitochondrial dysfunction. However, the relationships between cognitive function decline and mitochondrial dysfunction in the microgravity environment have not been elucidated. In this study, we simulated the microgravity environment in the Sprague-Dawley (SD) rats by -30° tail suspension for 28 days. We then investigated the changes of mitochondrial morphology and proteomics in the hippocampus. The electron microscopy results showed that the 28-day tail suspension increased the mitochondria number and size of rat hippocampal neuronal soma. Using TMT-based proteomics analysis, we identified 163 differentially expressed proteins (DEPs) between tail suspension and control samples, and among them, 128 proteins were upregulated and 35 proteins were downregulated. Functional and network analyses of the DEPs indicated that several of mitochondrial metabolic processes including the tricarboxylic acid (TCA) cycle were altered by simulating microgravity (SM). We verified 3 upregulated proteins, aconitate hydratase (ACO2), dihydrolipoamide S-succinyltransferase (DLST), and citrate synthase (CS), in the TCA cycle process by western blotting and confirmed their differential expressions between tail suspension and control samples. Taken together, our results demonstrate that 28-day tail suspension can cause changes in the morphology and metabolic function of hippocampus mitochondria, which might represent a mechanism of cognitive disorder caused by aerospace microgravity. Public Library of Science 2022-03-10 /pmc/articles/PMC8912132/ /pubmed/35271667 http://dx.doi.org/10.1371/journal.pone.0265108 Text en © 2022 Ji et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ji, Guohua Chang, Hui Yang, Mingsi Chen, Hailong Wang, Tingmei Liu, Xu Lv, Ke Li, Yinghui Song, Bo Qu, Lina The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity |
title | The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity |
title_full | The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity |
title_fullStr | The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity |
title_full_unstemmed | The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity |
title_short | The mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity |
title_sort | mitochondrial proteomic changes of rat hippocampus induced by 28-day simulated microgravity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912132/ https://www.ncbi.nlm.nih.gov/pubmed/35271667 http://dx.doi.org/10.1371/journal.pone.0265108 |
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