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Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and rhAmpSeq Genetic Map
Increased map density and transferability of markers are essential for the genetic analysis of fruit quality and stress tolerance in interspecific grapevine populations. We used 1449 GBS and 2000 rhAmpSeq markers to develop a dense map for an interspecific F(2) population (VRS-F(2)) that was derived...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912348/ https://www.ncbi.nlm.nih.gov/pubmed/35270166 http://dx.doi.org/10.3390/plants11050696 |
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author | Alahakoon, Dilmini Fennell, Anne Helget, Zachary Bates, Terry Karn, Avinash Manns, David Mansfield, Anna Katharine Reisch, Bruce I. Sacks, Gavin Sun, Qi Zou, Cheng Cadle-Davidson, Lance Londo, Jason P. |
author_facet | Alahakoon, Dilmini Fennell, Anne Helget, Zachary Bates, Terry Karn, Avinash Manns, David Mansfield, Anna Katharine Reisch, Bruce I. Sacks, Gavin Sun, Qi Zou, Cheng Cadle-Davidson, Lance Londo, Jason P. |
author_sort | Alahakoon, Dilmini |
collection | PubMed |
description | Increased map density and transferability of markers are essential for the genetic analysis of fruit quality and stress tolerance in interspecific grapevine populations. We used 1449 GBS and 2000 rhAmpSeq markers to develop a dense map for an interspecific F(2) population (VRS-F(2)) that was derived by selfing a single F(1) from a Vitis riparia x ‘Seyval blanc’ cross. The resultant map contained 2519 markers spanning 1131.3 cM and was highly collinear with the Vitis vinifera ‘PN40024’ genome. Quantitative trait loci (QTL) for berry skin color and flower type were used to validate the map. Four rhAmpSeq transferable markers were identified that can be used in pairs (one pistillate and one hermaphroditic) to predict pistillate and hermaphrodite flower type with ≥99.7% accuracy. Total and individual anthocyanin diglucoside QTL mapped to chromosome 9 near a 5-O-GLUCOSYLTRANSFERASE candidate gene. Malic acid QTL were observed on chromosome 1 and 6 with two MALATE DEHYRDROGENASE CYTOPLASMIC 1 and ALUMINUM-ACTIVATED MALATE TRANSPORTER 2-LIKE (ALMT) candidate genes, respectively. Modeling malic acid identified a potential QTL on chromosome 8 with peak position in proximity of another ALMT. A first-ever reported QTL for the grassy smelling volatile (E)-2-hexenal was found on chromosome 2 with a PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE candidate gene near peak markers. |
format | Online Article Text |
id | pubmed-8912348 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89123482022-03-11 Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and rhAmpSeq Genetic Map Alahakoon, Dilmini Fennell, Anne Helget, Zachary Bates, Terry Karn, Avinash Manns, David Mansfield, Anna Katharine Reisch, Bruce I. Sacks, Gavin Sun, Qi Zou, Cheng Cadle-Davidson, Lance Londo, Jason P. Plants (Basel) Article Increased map density and transferability of markers are essential for the genetic analysis of fruit quality and stress tolerance in interspecific grapevine populations. We used 1449 GBS and 2000 rhAmpSeq markers to develop a dense map for an interspecific F(2) population (VRS-F(2)) that was derived by selfing a single F(1) from a Vitis riparia x ‘Seyval blanc’ cross. The resultant map contained 2519 markers spanning 1131.3 cM and was highly collinear with the Vitis vinifera ‘PN40024’ genome. Quantitative trait loci (QTL) for berry skin color and flower type were used to validate the map. Four rhAmpSeq transferable markers were identified that can be used in pairs (one pistillate and one hermaphroditic) to predict pistillate and hermaphrodite flower type with ≥99.7% accuracy. Total and individual anthocyanin diglucoside QTL mapped to chromosome 9 near a 5-O-GLUCOSYLTRANSFERASE candidate gene. Malic acid QTL were observed on chromosome 1 and 6 with two MALATE DEHYRDROGENASE CYTOPLASMIC 1 and ALUMINUM-ACTIVATED MALATE TRANSPORTER 2-LIKE (ALMT) candidate genes, respectively. Modeling malic acid identified a potential QTL on chromosome 8 with peak position in proximity of another ALMT. A first-ever reported QTL for the grassy smelling volatile (E)-2-hexenal was found on chromosome 2 with a PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE candidate gene near peak markers. MDPI 2022-03-04 /pmc/articles/PMC8912348/ /pubmed/35270166 http://dx.doi.org/10.3390/plants11050696 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Alahakoon, Dilmini Fennell, Anne Helget, Zachary Bates, Terry Karn, Avinash Manns, David Mansfield, Anna Katharine Reisch, Bruce I. Sacks, Gavin Sun, Qi Zou, Cheng Cadle-Davidson, Lance Londo, Jason P. Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and rhAmpSeq Genetic Map |
title | Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and rhAmpSeq Genetic Map |
title_full | Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and rhAmpSeq Genetic Map |
title_fullStr | Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and rhAmpSeq Genetic Map |
title_full_unstemmed | Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and rhAmpSeq Genetic Map |
title_short | Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and rhAmpSeq Genetic Map |
title_sort | berry anthocyanin, acid, and volatile trait analyses in a grapevine-interspecific f2 population using an integrated gbs and rhampseq genetic map |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912348/ https://www.ncbi.nlm.nih.gov/pubmed/35270166 http://dx.doi.org/10.3390/plants11050696 |
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