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Comparisons of Anatomical Characteristics and Transcriptomic Differences between Heterografts and Homografts in Pyrus L.

Pear (Pyrus L.) is an important temperate fruit worldwide, and grafting is widely used in pear vegetative propagation. However, the mechanisms of graft healing or incompatibility remain poorly understood in Pyrus. To study the differences in graft healing in Pyrus, the homograft “Qingzhen D1/Qingzhe...

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Autores principales: Ji, Piyu, Liang, Chenglin, Yang, Yingjie, Wang, Ran, Wang, Yue, Yuan, Meitong, Qiu, Zhiyun, Cheng, Yuanyuan, Liu, Jianlong, Li, Dingli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912356/
https://www.ncbi.nlm.nih.gov/pubmed/35270050
http://dx.doi.org/10.3390/plants11050580
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author Ji, Piyu
Liang, Chenglin
Yang, Yingjie
Wang, Ran
Wang, Yue
Yuan, Meitong
Qiu, Zhiyun
Cheng, Yuanyuan
Liu, Jianlong
Li, Dingli
author_facet Ji, Piyu
Liang, Chenglin
Yang, Yingjie
Wang, Ran
Wang, Yue
Yuan, Meitong
Qiu, Zhiyun
Cheng, Yuanyuan
Liu, Jianlong
Li, Dingli
author_sort Ji, Piyu
collection PubMed
description Pear (Pyrus L.) is an important temperate fruit worldwide, and grafting is widely used in pear vegetative propagation. However, the mechanisms of graft healing or incompatibility remain poorly understood in Pyrus. To study the differences in graft healing in Pyrus, the homograft “Qingzhen D1/Qingzhen D1” and the heterograft “QAUP-1/Qingzhen D1” as compatibility and incompatibility combinations were compared. Anatomical differences indicated the healing process was faster in homografts than in heterografts. During the healing process, four critical stages in graft union formation were identified in the two types of grafts. The expression of the genes associated with hormone signaling (auxin and cytokinins), and lignin biosynthesis was delayed in the healing process of heterografts. In addition, the PbBglu13 gene, encoded β-glucosidase, was more highly up-regulated in heterografts than in homografts to promote healing. Meanwhile, the most of DEGs related starch and sucrose metabolism were found to be up-regulated in heterografts; those results indicated that cellulose and sugar signals were also involved in graft healing. The results of this study improved the understanding of the differences in the mechanisms of graft healing between homografts and heterografts.
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spelling pubmed-89123562022-03-11 Comparisons of Anatomical Characteristics and Transcriptomic Differences between Heterografts and Homografts in Pyrus L. Ji, Piyu Liang, Chenglin Yang, Yingjie Wang, Ran Wang, Yue Yuan, Meitong Qiu, Zhiyun Cheng, Yuanyuan Liu, Jianlong Li, Dingli Plants (Basel) Article Pear (Pyrus L.) is an important temperate fruit worldwide, and grafting is widely used in pear vegetative propagation. However, the mechanisms of graft healing or incompatibility remain poorly understood in Pyrus. To study the differences in graft healing in Pyrus, the homograft “Qingzhen D1/Qingzhen D1” and the heterograft “QAUP-1/Qingzhen D1” as compatibility and incompatibility combinations were compared. Anatomical differences indicated the healing process was faster in homografts than in heterografts. During the healing process, four critical stages in graft union formation were identified in the two types of grafts. The expression of the genes associated with hormone signaling (auxin and cytokinins), and lignin biosynthesis was delayed in the healing process of heterografts. In addition, the PbBglu13 gene, encoded β-glucosidase, was more highly up-regulated in heterografts than in homografts to promote healing. Meanwhile, the most of DEGs related starch and sucrose metabolism were found to be up-regulated in heterografts; those results indicated that cellulose and sugar signals were also involved in graft healing. The results of this study improved the understanding of the differences in the mechanisms of graft healing between homografts and heterografts. MDPI 2022-02-22 /pmc/articles/PMC8912356/ /pubmed/35270050 http://dx.doi.org/10.3390/plants11050580 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ji, Piyu
Liang, Chenglin
Yang, Yingjie
Wang, Ran
Wang, Yue
Yuan, Meitong
Qiu, Zhiyun
Cheng, Yuanyuan
Liu, Jianlong
Li, Dingli
Comparisons of Anatomical Characteristics and Transcriptomic Differences between Heterografts and Homografts in Pyrus L.
title Comparisons of Anatomical Characteristics and Transcriptomic Differences between Heterografts and Homografts in Pyrus L.
title_full Comparisons of Anatomical Characteristics and Transcriptomic Differences between Heterografts and Homografts in Pyrus L.
title_fullStr Comparisons of Anatomical Characteristics and Transcriptomic Differences between Heterografts and Homografts in Pyrus L.
title_full_unstemmed Comparisons of Anatomical Characteristics and Transcriptomic Differences between Heterografts and Homografts in Pyrus L.
title_short Comparisons of Anatomical Characteristics and Transcriptomic Differences between Heterografts and Homografts in Pyrus L.
title_sort comparisons of anatomical characteristics and transcriptomic differences between heterografts and homografts in pyrus l.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912356/
https://www.ncbi.nlm.nih.gov/pubmed/35270050
http://dx.doi.org/10.3390/plants11050580
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