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Anti-Adhesion and Antibiofilm Activity of Eruca sativa Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach

Bacterial cells have the ability to form biofilm onto the surfaces of food matrixes and on food processing equipment, leading to a source of food contamination posing serious health implications. Therefore, our study aimed to determine the effect of Eruca sativa Miller (E. sativa) crude extract agai...

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Autores principales: Awadelkareem, Amir Mahgoub, Al-Shammari, Eyad, Elkhalifa, AbdElmoneim O., Adnan, Mohd, Siddiqui, Arif Jamal, Mahmood, Danish, Azad, Z. R. Azaz Ahmad, Patel, Mitesh, Mehmood, Khalid, Danciu, Corina, Ashraf, Syed Amir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912376/
https://www.ncbi.nlm.nih.gov/pubmed/35270080
http://dx.doi.org/10.3390/plants11050610
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author Awadelkareem, Amir Mahgoub
Al-Shammari, Eyad
Elkhalifa, AbdElmoneim O.
Adnan, Mohd
Siddiqui, Arif Jamal
Mahmood, Danish
Azad, Z. R. Azaz Ahmad
Patel, Mitesh
Mehmood, Khalid
Danciu, Corina
Ashraf, Syed Amir
author_facet Awadelkareem, Amir Mahgoub
Al-Shammari, Eyad
Elkhalifa, AbdElmoneim O.
Adnan, Mohd
Siddiqui, Arif Jamal
Mahmood, Danish
Azad, Z. R. Azaz Ahmad
Patel, Mitesh
Mehmood, Khalid
Danciu, Corina
Ashraf, Syed Amir
author_sort Awadelkareem, Amir Mahgoub
collection PubMed
description Bacterial cells have the ability to form biofilm onto the surfaces of food matrixes and on food processing equipment, leading to a source of food contamination posing serious health implications. Therefore, our study aimed to determine the effect of Eruca sativa Miller (E. sativa) crude extract against biofilms of food-borne bacteria along with in silico approaches to investigate adhesion proteins responsible for biofilm activity against the identified phytochemicals. The antibacterial potential of crude extract was evaluated using agar well diffusion technique and combinations of light and scanning electron microscopy to assess the efficacy of crude extract against the developed biofilms. Our results showed that crude extract of E. sativa was active against all tested food-borne bacteria, exhibiting a rapid kinetics of killing bacteria in a time-dependent manner. MIC and MBC values of E. sativa crude extract were found to be ranging from 125 to 500 µg/mL and 250 to 1000 µg/mL respectively. Furthermore, inhibition of developed biofilm by E sativa was found to be ranging from 58.68% to 73.45% for all the tested strains. The crude extract also reduced the viability of bacterial cells within biofilms and amount of EPS (ranging 59.73–82.77%) in the biofilm matrix. Additionally, the microscopic images also revealed significant disruption in the structure of biofilms. A molecular docking analysis of E. sativa phytochemicals showed interaction with active site of adhesion proteins Sortase A, EspA, OprD, and type IV b pilin of S. aureus, E. coli, P. aeruginosa, and S. enterica ser. typhi, respectively. Thus, our findings represent the first demonstration of E. sativa crude extract’s bioactivity and potency against food-borne bacteria in their planktonic forms, as well as against the developed biofilms. Therefore, a possible mechanistic approach for inhibition of biofilm via targeting adhesion proteins can be explored further to target biofilm producing food-borne bacterial pathogens.
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spelling pubmed-89123762022-03-11 Anti-Adhesion and Antibiofilm Activity of Eruca sativa Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach Awadelkareem, Amir Mahgoub Al-Shammari, Eyad Elkhalifa, AbdElmoneim O. Adnan, Mohd Siddiqui, Arif Jamal Mahmood, Danish Azad, Z. R. Azaz Ahmad Patel, Mitesh Mehmood, Khalid Danciu, Corina Ashraf, Syed Amir Plants (Basel) Article Bacterial cells have the ability to form biofilm onto the surfaces of food matrixes and on food processing equipment, leading to a source of food contamination posing serious health implications. Therefore, our study aimed to determine the effect of Eruca sativa Miller (E. sativa) crude extract against biofilms of food-borne bacteria along with in silico approaches to investigate adhesion proteins responsible for biofilm activity against the identified phytochemicals. The antibacterial potential of crude extract was evaluated using agar well diffusion technique and combinations of light and scanning electron microscopy to assess the efficacy of crude extract against the developed biofilms. Our results showed that crude extract of E. sativa was active against all tested food-borne bacteria, exhibiting a rapid kinetics of killing bacteria in a time-dependent manner. MIC and MBC values of E. sativa crude extract were found to be ranging from 125 to 500 µg/mL and 250 to 1000 µg/mL respectively. Furthermore, inhibition of developed biofilm by E sativa was found to be ranging from 58.68% to 73.45% for all the tested strains. The crude extract also reduced the viability of bacterial cells within biofilms and amount of EPS (ranging 59.73–82.77%) in the biofilm matrix. Additionally, the microscopic images also revealed significant disruption in the structure of biofilms. A molecular docking analysis of E. sativa phytochemicals showed interaction with active site of adhesion proteins Sortase A, EspA, OprD, and type IV b pilin of S. aureus, E. coli, P. aeruginosa, and S. enterica ser. typhi, respectively. Thus, our findings represent the first demonstration of E. sativa crude extract’s bioactivity and potency against food-borne bacteria in their planktonic forms, as well as against the developed biofilms. Therefore, a possible mechanistic approach for inhibition of biofilm via targeting adhesion proteins can be explored further to target biofilm producing food-borne bacterial pathogens. MDPI 2022-02-24 /pmc/articles/PMC8912376/ /pubmed/35270080 http://dx.doi.org/10.3390/plants11050610 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Awadelkareem, Amir Mahgoub
Al-Shammari, Eyad
Elkhalifa, AbdElmoneim O.
Adnan, Mohd
Siddiqui, Arif Jamal
Mahmood, Danish
Azad, Z. R. Azaz Ahmad
Patel, Mitesh
Mehmood, Khalid
Danciu, Corina
Ashraf, Syed Amir
Anti-Adhesion and Antibiofilm Activity of Eruca sativa Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach
title Anti-Adhesion and Antibiofilm Activity of Eruca sativa Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach
title_full Anti-Adhesion and Antibiofilm Activity of Eruca sativa Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach
title_fullStr Anti-Adhesion and Antibiofilm Activity of Eruca sativa Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach
title_full_unstemmed Anti-Adhesion and Antibiofilm Activity of Eruca sativa Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach
title_short Anti-Adhesion and Antibiofilm Activity of Eruca sativa Miller Extract Targeting Cell Adhesion Proteins of Food-Borne Bacteria as a Potential Mechanism: Combined In Vitro-In Silico Approach
title_sort anti-adhesion and antibiofilm activity of eruca sativa miller extract targeting cell adhesion proteins of food-borne bacteria as a potential mechanism: combined in vitro-in silico approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912376/
https://www.ncbi.nlm.nih.gov/pubmed/35270080
http://dx.doi.org/10.3390/plants11050610
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