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Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Solanum tuberosum L.) Plants in Response to Potato Virus Y Infection

Plant-virus interactions are greatly influenced by environmental factors such as temperatures. In virus-infected plants, enhanced temperature is frequently associated with more severe symptoms and higher virus content. However, the mechanisms involved in controlling the temperature regulation of pla...

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Autores principales: Glushkevich, Anna, Spechenkova, Nadezhda, Fesenko, Igor, Knyazev, Andrey, Samarskaya, Viktoriya, Kalinina, Natalia O., Taliansky, Michael, Love, Andrew J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912425/
https://www.ncbi.nlm.nih.gov/pubmed/35270104
http://dx.doi.org/10.3390/plants11050635
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author Glushkevich, Anna
Spechenkova, Nadezhda
Fesenko, Igor
Knyazev, Andrey
Samarskaya, Viktoriya
Kalinina, Natalia O.
Taliansky, Michael
Love, Andrew J.
author_facet Glushkevich, Anna
Spechenkova, Nadezhda
Fesenko, Igor
Knyazev, Andrey
Samarskaya, Viktoriya
Kalinina, Natalia O.
Taliansky, Michael
Love, Andrew J.
author_sort Glushkevich, Anna
collection PubMed
description Plant-virus interactions are greatly influenced by environmental factors such as temperatures. In virus-infected plants, enhanced temperature is frequently associated with more severe symptoms and higher virus content. However, the mechanisms involved in controlling the temperature regulation of plant-virus interactions are poorly characterised. To elucidate these further, we analysed the responses of potato plants cv Chicago to infection by potato virus Y (PVY) at normal (22 °C) and elevated temperature (28 °C), the latter of which is known to significantly increase plant susceptibility to PVY. Using RNAseq analysis, we showed that single and combined PVY and heat-stress treatments caused dramatic changes in gene expression, affecting the transcription of both protein-coding and non-coding RNAs. Among the newly identified genes responsive to PVY infection, we found genes encoding enzymes involved in the catalysis of polyamine formation and poly ADP-ribosylation. We also identified a range of novel non-coding RNAs which were differentially produced in response to single or combined PVY and heat stress, that consisted of antisense RNAs and RNAs with miRNA binding sites. Finally, to gain more insights into the potential role of alternative splicing and epitranscriptomic RNA methylation during combined stress conditions, direct RNA nanopore sequencing was performed. Our findings offer insights for future studies of functional links between virus infections and transcriptome reprogramming, RNA methylation and alternative splicing.
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spelling pubmed-89124252022-03-11 Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Solanum tuberosum L.) Plants in Response to Potato Virus Y Infection Glushkevich, Anna Spechenkova, Nadezhda Fesenko, Igor Knyazev, Andrey Samarskaya, Viktoriya Kalinina, Natalia O. Taliansky, Michael Love, Andrew J. Plants (Basel) Article Plant-virus interactions are greatly influenced by environmental factors such as temperatures. In virus-infected plants, enhanced temperature is frequently associated with more severe symptoms and higher virus content. However, the mechanisms involved in controlling the temperature regulation of plant-virus interactions are poorly characterised. To elucidate these further, we analysed the responses of potato plants cv Chicago to infection by potato virus Y (PVY) at normal (22 °C) and elevated temperature (28 °C), the latter of which is known to significantly increase plant susceptibility to PVY. Using RNAseq analysis, we showed that single and combined PVY and heat-stress treatments caused dramatic changes in gene expression, affecting the transcription of both protein-coding and non-coding RNAs. Among the newly identified genes responsive to PVY infection, we found genes encoding enzymes involved in the catalysis of polyamine formation and poly ADP-ribosylation. We also identified a range of novel non-coding RNAs which were differentially produced in response to single or combined PVY and heat stress, that consisted of antisense RNAs and RNAs with miRNA binding sites. Finally, to gain more insights into the potential role of alternative splicing and epitranscriptomic RNA methylation during combined stress conditions, direct RNA nanopore sequencing was performed. Our findings offer insights for future studies of functional links between virus infections and transcriptome reprogramming, RNA methylation and alternative splicing. MDPI 2022-02-25 /pmc/articles/PMC8912425/ /pubmed/35270104 http://dx.doi.org/10.3390/plants11050635 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Glushkevich, Anna
Spechenkova, Nadezhda
Fesenko, Igor
Knyazev, Andrey
Samarskaya, Viktoriya
Kalinina, Natalia O.
Taliansky, Michael
Love, Andrew J.
Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Solanum tuberosum L.) Plants in Response to Potato Virus Y Infection
title Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Solanum tuberosum L.) Plants in Response to Potato Virus Y Infection
title_full Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Solanum tuberosum L.) Plants in Response to Potato Virus Y Infection
title_fullStr Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Solanum tuberosum L.) Plants in Response to Potato Virus Y Infection
title_full_unstemmed Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Solanum tuberosum L.) Plants in Response to Potato Virus Y Infection
title_short Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Solanum tuberosum L.) Plants in Response to Potato Virus Y Infection
title_sort transcriptomic reprogramming, alternative splicing and rna methylation in potato (solanum tuberosum l.) plants in response to potato virus y infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912425/
https://www.ncbi.nlm.nih.gov/pubmed/35270104
http://dx.doi.org/10.3390/plants11050635
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