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Genome-Wide Analysis of the Banana WRKY Transcription Factor Gene Family Closely Related to Fruit Ripening and Stress

WRKY transcription factors (TFs) play an important role in plant responses to biotic and abiotic stress as well as in plant growth and development. In the present study, bioinformatics methods were used to identify members of the WRKY transcription factor family in the Musa acuminata (DH-Pahang) gen...

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Autores principales: Jia, Caihong, Wang, Zhuo, Wang, Jingyi, Miao, Hongxia, Zhang, Jianbin, Xu, Biyu, Liu, Juhua, Jin, Zhiqiang, Liu, Jihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912484/
https://www.ncbi.nlm.nih.gov/pubmed/35270130
http://dx.doi.org/10.3390/plants11050662
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author Jia, Caihong
Wang, Zhuo
Wang, Jingyi
Miao, Hongxia
Zhang, Jianbin
Xu, Biyu
Liu, Juhua
Jin, Zhiqiang
Liu, Jihong
author_facet Jia, Caihong
Wang, Zhuo
Wang, Jingyi
Miao, Hongxia
Zhang, Jianbin
Xu, Biyu
Liu, Juhua
Jin, Zhiqiang
Liu, Jihong
author_sort Jia, Caihong
collection PubMed
description WRKY transcription factors (TFs) play an important role in plant responses to biotic and abiotic stress as well as in plant growth and development. In the present study, bioinformatics methods were used to identify members of the WRKY transcription factor family in the Musa acuminata (DH-Pahang) genome (version 2). A total of 164 MaWRKYs were identified and phylogenetic analysis showed that MaWRKYs could be categorized into three subfamilies. Overall, the 162 MaWRKYs were distributed on 11 chromosomes, and 2 genes were not located on the chromosome. There were 31 collinear genes from segmental duplication and 7 pairs of genes from tandem duplication. RNA-sequencing was used to analyze the expression profiles of MaWRKYs in different fruit development, ripening stages, under various abiotic and biotic stressors. Most of the MaWRKYs showed a variety of expression patterns in the banana fruit development and ripening stages. Some MaWRKYs responded to abiotic stress, such as low temperature, drought, and salt stress. Most differentially expressed MaWRKYs were downregulated during banana’s response to Foc TR4 infection, which plays an important role in physiological regulation to stress. Our findings indicate that MaWRKY21 directly binds to the W-box of the MaICS promoter to decrease MaICS transcription and then reduce the enzyme activity. These studies have improved our understanding of the molecular basis for the development and stress resistance of an important banana variety.
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spelling pubmed-89124842022-03-11 Genome-Wide Analysis of the Banana WRKY Transcription Factor Gene Family Closely Related to Fruit Ripening and Stress Jia, Caihong Wang, Zhuo Wang, Jingyi Miao, Hongxia Zhang, Jianbin Xu, Biyu Liu, Juhua Jin, Zhiqiang Liu, Jihong Plants (Basel) Article WRKY transcription factors (TFs) play an important role in plant responses to biotic and abiotic stress as well as in plant growth and development. In the present study, bioinformatics methods were used to identify members of the WRKY transcription factor family in the Musa acuminata (DH-Pahang) genome (version 2). A total of 164 MaWRKYs were identified and phylogenetic analysis showed that MaWRKYs could be categorized into three subfamilies. Overall, the 162 MaWRKYs were distributed on 11 chromosomes, and 2 genes were not located on the chromosome. There were 31 collinear genes from segmental duplication and 7 pairs of genes from tandem duplication. RNA-sequencing was used to analyze the expression profiles of MaWRKYs in different fruit development, ripening stages, under various abiotic and biotic stressors. Most of the MaWRKYs showed a variety of expression patterns in the banana fruit development and ripening stages. Some MaWRKYs responded to abiotic stress, such as low temperature, drought, and salt stress. Most differentially expressed MaWRKYs were downregulated during banana’s response to Foc TR4 infection, which plays an important role in physiological regulation to stress. Our findings indicate that MaWRKY21 directly binds to the W-box of the MaICS promoter to decrease MaICS transcription and then reduce the enzyme activity. These studies have improved our understanding of the molecular basis for the development and stress resistance of an important banana variety. MDPI 2022-02-28 /pmc/articles/PMC8912484/ /pubmed/35270130 http://dx.doi.org/10.3390/plants11050662 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jia, Caihong
Wang, Zhuo
Wang, Jingyi
Miao, Hongxia
Zhang, Jianbin
Xu, Biyu
Liu, Juhua
Jin, Zhiqiang
Liu, Jihong
Genome-Wide Analysis of the Banana WRKY Transcription Factor Gene Family Closely Related to Fruit Ripening and Stress
title Genome-Wide Analysis of the Banana WRKY Transcription Factor Gene Family Closely Related to Fruit Ripening and Stress
title_full Genome-Wide Analysis of the Banana WRKY Transcription Factor Gene Family Closely Related to Fruit Ripening and Stress
title_fullStr Genome-Wide Analysis of the Banana WRKY Transcription Factor Gene Family Closely Related to Fruit Ripening and Stress
title_full_unstemmed Genome-Wide Analysis of the Banana WRKY Transcription Factor Gene Family Closely Related to Fruit Ripening and Stress
title_short Genome-Wide Analysis of the Banana WRKY Transcription Factor Gene Family Closely Related to Fruit Ripening and Stress
title_sort genome-wide analysis of the banana wrky transcription factor gene family closely related to fruit ripening and stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912484/
https://www.ncbi.nlm.nih.gov/pubmed/35270130
http://dx.doi.org/10.3390/plants11050662
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