Cargando…

Ellagic Acid Alleviates Diquat-Induced Jejunum Oxidative Stress in C57BL/6 Mice through Activating Nrf2 Mediated Signaling Pathway

Ellagic acid (EA) is the main constituent found in pomegranate rind, which has anti-inflammatory and antioxidant effects. However, whether EA can alleviate diquat-induced oxidative stress is still unknown. Here, the effects and mechanisms of EA on jejunum oxidative stress induced by diquat was inves...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Xiangyu, Wang, Shilan, Wu, Yujun, Liu, Xiaoyi, Wang, Junjun, Han, Dandan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912502/
https://www.ncbi.nlm.nih.gov/pubmed/35268077
http://dx.doi.org/10.3390/nu14051103
_version_ 1784667150552662016
author Zhang, Xiangyu
Wang, Shilan
Wu, Yujun
Liu, Xiaoyi
Wang, Junjun
Han, Dandan
author_facet Zhang, Xiangyu
Wang, Shilan
Wu, Yujun
Liu, Xiaoyi
Wang, Junjun
Han, Dandan
author_sort Zhang, Xiangyu
collection PubMed
description Ellagic acid (EA) is the main constituent found in pomegranate rind, which has anti-inflammatory and antioxidant effects. However, whether EA can alleviate diquat-induced oxidative stress is still unknown. Here, the effects and mechanisms of EA on jejunum oxidative stress induced by diquat was investigated. Oxidative stress was induced in mice by administrating diquat (25 mg/kg body weight) followed by treatment with 100 mg/kg body weight EA for 5 days. Results showed that oral administration of EA significantly ameliorated diquat-induced weight loss and oxidative stress (p < 0.05) evidenced by reduced ROS production in the jejunum. Furthermore, EA up-regulated the mRNA expression of the antioxidant enzymes (Nrf2, GPX1 and HO-1) when mice were challenged with diquat, compared with the diquat group (p < 0.05). Importantly, pharmacological inhibition of Nrf2 by ML385 counteracted the EA-mediated alleviation of jejunum oxidative stress, as evidence by body weight and ROS production. Also, immunohistochemistry staining confirmed the markedly decreased jejunal Nrf2 expression. The up-regulated effect on NQO1 and HO-1 mRNA expression induced by EA was diminished in mice treated with ML385 (p < 0.05). Together, our results demonstrated that therapeutic and preventative EA treatment was effective in reducing weight loss and oxidative stress induced by diquat through the Nrf2 mediated signaling pathway.
format Online
Article
Text
id pubmed-8912502
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89125022022-03-11 Ellagic Acid Alleviates Diquat-Induced Jejunum Oxidative Stress in C57BL/6 Mice through Activating Nrf2 Mediated Signaling Pathway Zhang, Xiangyu Wang, Shilan Wu, Yujun Liu, Xiaoyi Wang, Junjun Han, Dandan Nutrients Article Ellagic acid (EA) is the main constituent found in pomegranate rind, which has anti-inflammatory and antioxidant effects. However, whether EA can alleviate diquat-induced oxidative stress is still unknown. Here, the effects and mechanisms of EA on jejunum oxidative stress induced by diquat was investigated. Oxidative stress was induced in mice by administrating diquat (25 mg/kg body weight) followed by treatment with 100 mg/kg body weight EA for 5 days. Results showed that oral administration of EA significantly ameliorated diquat-induced weight loss and oxidative stress (p < 0.05) evidenced by reduced ROS production in the jejunum. Furthermore, EA up-regulated the mRNA expression of the antioxidant enzymes (Nrf2, GPX1 and HO-1) when mice were challenged with diquat, compared with the diquat group (p < 0.05). Importantly, pharmacological inhibition of Nrf2 by ML385 counteracted the EA-mediated alleviation of jejunum oxidative stress, as evidence by body weight and ROS production. Also, immunohistochemistry staining confirmed the markedly decreased jejunal Nrf2 expression. The up-regulated effect on NQO1 and HO-1 mRNA expression induced by EA was diminished in mice treated with ML385 (p < 0.05). Together, our results demonstrated that therapeutic and preventative EA treatment was effective in reducing weight loss and oxidative stress induced by diquat through the Nrf2 mediated signaling pathway. MDPI 2022-03-05 /pmc/articles/PMC8912502/ /pubmed/35268077 http://dx.doi.org/10.3390/nu14051103 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Xiangyu
Wang, Shilan
Wu, Yujun
Liu, Xiaoyi
Wang, Junjun
Han, Dandan
Ellagic Acid Alleviates Diquat-Induced Jejunum Oxidative Stress in C57BL/6 Mice through Activating Nrf2 Mediated Signaling Pathway
title Ellagic Acid Alleviates Diquat-Induced Jejunum Oxidative Stress in C57BL/6 Mice through Activating Nrf2 Mediated Signaling Pathway
title_full Ellagic Acid Alleviates Diquat-Induced Jejunum Oxidative Stress in C57BL/6 Mice through Activating Nrf2 Mediated Signaling Pathway
title_fullStr Ellagic Acid Alleviates Diquat-Induced Jejunum Oxidative Stress in C57BL/6 Mice through Activating Nrf2 Mediated Signaling Pathway
title_full_unstemmed Ellagic Acid Alleviates Diquat-Induced Jejunum Oxidative Stress in C57BL/6 Mice through Activating Nrf2 Mediated Signaling Pathway
title_short Ellagic Acid Alleviates Diquat-Induced Jejunum Oxidative Stress in C57BL/6 Mice through Activating Nrf2 Mediated Signaling Pathway
title_sort ellagic acid alleviates diquat-induced jejunum oxidative stress in c57bl/6 mice through activating nrf2 mediated signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912502/
https://www.ncbi.nlm.nih.gov/pubmed/35268077
http://dx.doi.org/10.3390/nu14051103
work_keys_str_mv AT zhangxiangyu ellagicacidalleviatesdiquatinducedjejunumoxidativestressinc57bl6micethroughactivatingnrf2mediatedsignalingpathway
AT wangshilan ellagicacidalleviatesdiquatinducedjejunumoxidativestressinc57bl6micethroughactivatingnrf2mediatedsignalingpathway
AT wuyujun ellagicacidalleviatesdiquatinducedjejunumoxidativestressinc57bl6micethroughactivatingnrf2mediatedsignalingpathway
AT liuxiaoyi ellagicacidalleviatesdiquatinducedjejunumoxidativestressinc57bl6micethroughactivatingnrf2mediatedsignalingpathway
AT wangjunjun ellagicacidalleviatesdiquatinducedjejunumoxidativestressinc57bl6micethroughactivatingnrf2mediatedsignalingpathway
AT handandan ellagicacidalleviatesdiquatinducedjejunumoxidativestressinc57bl6micethroughactivatingnrf2mediatedsignalingpathway