Cargando…
Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats
The opioid antagonist naltrexone (NTX) decreases intake of preferred diets in rats at very low doses relative to doses needed to decrease intake of “bland” laboratory chow. In the absence of an opioid agonist, NTX is not discriminable using operant techniques. In the current study, we found that rat...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912831/ https://www.ncbi.nlm.nih.gov/pubmed/35267900 http://dx.doi.org/10.3390/nu14050926 |
_version_ | 1784667257481199616 |
---|---|
author | Jewett, David C. Liyanagamage, Donisha S. N. K. Avond, Mark A. Vanden Anderson, Molly A. B. Twaroski, Kyleigh A. Marek, Morgan A. James, Kimberly F. Pal, Tapasya Klockars, Anica Olszewski, Pawel K. Levine, Allen S. |
author_facet | Jewett, David C. Liyanagamage, Donisha S. N. K. Avond, Mark A. Vanden Anderson, Molly A. B. Twaroski, Kyleigh A. Marek, Morgan A. James, Kimberly F. Pal, Tapasya Klockars, Anica Olszewski, Pawel K. Levine, Allen S. |
author_sort | Jewett, David C. |
collection | PubMed |
description | The opioid antagonist naltrexone (NTX) decreases intake of preferred diets in rats at very low doses relative to doses needed to decrease intake of “bland” laboratory chow. In the absence of an opioid agonist, NTX is not discriminable using operant techniques. In the current study, we found that rats given intermittent access to a 25% sucrose solution learned to discriminate between various naltrexone doses and saline. None of the rats given only water learned to discriminate between naltrexone and saline. When access to the sucrose solution was discontinued for 14 days, the rats lost the ability to discriminate between NTX and saline. We also studied the changes of c-Fos IR in selected brain regions in rats treated with saline versus NTX that were drinking water or 25% sucrose. An injection of NTX or saline resulted in a significant drug, diet, and interaction effect in various brain regions associated with feeding behavior, particularly the amygdala, accumbens, and hypothalamic sites. Thus, we found that ingestion of a sucrose solution results in the ability of rats to reliably discriminate naltrexone administration. In addition, sucrose and naltrexone altered c-Fos IR in an interactive fashion in brain regions known to be involved in ingestion behavior. |
format | Online Article Text |
id | pubmed-8912831 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89128312022-03-11 Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats Jewett, David C. Liyanagamage, Donisha S. N. K. Avond, Mark A. Vanden Anderson, Molly A. B. Twaroski, Kyleigh A. Marek, Morgan A. James, Kimberly F. Pal, Tapasya Klockars, Anica Olszewski, Pawel K. Levine, Allen S. Nutrients Article The opioid antagonist naltrexone (NTX) decreases intake of preferred diets in rats at very low doses relative to doses needed to decrease intake of “bland” laboratory chow. In the absence of an opioid agonist, NTX is not discriminable using operant techniques. In the current study, we found that rats given intermittent access to a 25% sucrose solution learned to discriminate between various naltrexone doses and saline. None of the rats given only water learned to discriminate between naltrexone and saline. When access to the sucrose solution was discontinued for 14 days, the rats lost the ability to discriminate between NTX and saline. We also studied the changes of c-Fos IR in selected brain regions in rats treated with saline versus NTX that were drinking water or 25% sucrose. An injection of NTX or saline resulted in a significant drug, diet, and interaction effect in various brain regions associated with feeding behavior, particularly the amygdala, accumbens, and hypothalamic sites. Thus, we found that ingestion of a sucrose solution results in the ability of rats to reliably discriminate naltrexone administration. In addition, sucrose and naltrexone altered c-Fos IR in an interactive fashion in brain regions known to be involved in ingestion behavior. MDPI 2022-02-22 /pmc/articles/PMC8912831/ /pubmed/35267900 http://dx.doi.org/10.3390/nu14050926 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jewett, David C. Liyanagamage, Donisha S. N. K. Avond, Mark A. Vanden Anderson, Molly A. B. Twaroski, Kyleigh A. Marek, Morgan A. James, Kimberly F. Pal, Tapasya Klockars, Anica Olszewski, Pawel K. Levine, Allen S. Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats |
title | Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats |
title_full | Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats |
title_fullStr | Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats |
title_full_unstemmed | Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats |
title_short | Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats |
title_sort | chronic intermittent sucrose consumption facilitates the ability to discriminate opioid receptor blockade with naltrexone in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912831/ https://www.ncbi.nlm.nih.gov/pubmed/35267900 http://dx.doi.org/10.3390/nu14050926 |
work_keys_str_mv | AT jewettdavidc chronicintermittentsucroseconsumptionfacilitatestheabilitytodiscriminateopioidreceptorblockadewithnaltrexoneinrats AT liyanagamagedonishasnk chronicintermittentsucroseconsumptionfacilitatestheabilitytodiscriminateopioidreceptorblockadewithnaltrexoneinrats AT avondmarkavanden chronicintermittentsucroseconsumptionfacilitatestheabilitytodiscriminateopioidreceptorblockadewithnaltrexoneinrats AT andersonmollyab chronicintermittentsucroseconsumptionfacilitatestheabilitytodiscriminateopioidreceptorblockadewithnaltrexoneinrats AT twaroskikyleigha chronicintermittentsucroseconsumptionfacilitatestheabilitytodiscriminateopioidreceptorblockadewithnaltrexoneinrats AT marekmorgana chronicintermittentsucroseconsumptionfacilitatestheabilitytodiscriminateopioidreceptorblockadewithnaltrexoneinrats AT jameskimberlyf chronicintermittentsucroseconsumptionfacilitatestheabilitytodiscriminateopioidreceptorblockadewithnaltrexoneinrats AT paltapasya chronicintermittentsucroseconsumptionfacilitatestheabilitytodiscriminateopioidreceptorblockadewithnaltrexoneinrats AT klockarsanica chronicintermittentsucroseconsumptionfacilitatestheabilitytodiscriminateopioidreceptorblockadewithnaltrexoneinrats AT olszewskipawelk chronicintermittentsucroseconsumptionfacilitatestheabilitytodiscriminateopioidreceptorblockadewithnaltrexoneinrats AT levineallens chronicintermittentsucroseconsumptionfacilitatestheabilitytodiscriminateopioidreceptorblockadewithnaltrexoneinrats |