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Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats

The opioid antagonist naltrexone (NTX) decreases intake of preferred diets in rats at very low doses relative to doses needed to decrease intake of “bland” laboratory chow. In the absence of an opioid agonist, NTX is not discriminable using operant techniques. In the current study, we found that rat...

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Autores principales: Jewett, David C., Liyanagamage, Donisha S. N. K., Avond, Mark A. Vanden, Anderson, Molly A. B., Twaroski, Kyleigh A., Marek, Morgan A., James, Kimberly F., Pal, Tapasya, Klockars, Anica, Olszewski, Pawel K., Levine, Allen S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912831/
https://www.ncbi.nlm.nih.gov/pubmed/35267900
http://dx.doi.org/10.3390/nu14050926
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author Jewett, David C.
Liyanagamage, Donisha S. N. K.
Avond, Mark A. Vanden
Anderson, Molly A. B.
Twaroski, Kyleigh A.
Marek, Morgan A.
James, Kimberly F.
Pal, Tapasya
Klockars, Anica
Olszewski, Pawel K.
Levine, Allen S.
author_facet Jewett, David C.
Liyanagamage, Donisha S. N. K.
Avond, Mark A. Vanden
Anderson, Molly A. B.
Twaroski, Kyleigh A.
Marek, Morgan A.
James, Kimberly F.
Pal, Tapasya
Klockars, Anica
Olszewski, Pawel K.
Levine, Allen S.
author_sort Jewett, David C.
collection PubMed
description The opioid antagonist naltrexone (NTX) decreases intake of preferred diets in rats at very low doses relative to doses needed to decrease intake of “bland” laboratory chow. In the absence of an opioid agonist, NTX is not discriminable using operant techniques. In the current study, we found that rats given intermittent access to a 25% sucrose solution learned to discriminate between various naltrexone doses and saline. None of the rats given only water learned to discriminate between naltrexone and saline. When access to the sucrose solution was discontinued for 14 days, the rats lost the ability to discriminate between NTX and saline. We also studied the changes of c-Fos IR in selected brain regions in rats treated with saline versus NTX that were drinking water or 25% sucrose. An injection of NTX or saline resulted in a significant drug, diet, and interaction effect in various brain regions associated with feeding behavior, particularly the amygdala, accumbens, and hypothalamic sites. Thus, we found that ingestion of a sucrose solution results in the ability of rats to reliably discriminate naltrexone administration. In addition, sucrose and naltrexone altered c-Fos IR in an interactive fashion in brain regions known to be involved in ingestion behavior.
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spelling pubmed-89128312022-03-11 Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats Jewett, David C. Liyanagamage, Donisha S. N. K. Avond, Mark A. Vanden Anderson, Molly A. B. Twaroski, Kyleigh A. Marek, Morgan A. James, Kimberly F. Pal, Tapasya Klockars, Anica Olszewski, Pawel K. Levine, Allen S. Nutrients Article The opioid antagonist naltrexone (NTX) decreases intake of preferred diets in rats at very low doses relative to doses needed to decrease intake of “bland” laboratory chow. In the absence of an opioid agonist, NTX is not discriminable using operant techniques. In the current study, we found that rats given intermittent access to a 25% sucrose solution learned to discriminate between various naltrexone doses and saline. None of the rats given only water learned to discriminate between naltrexone and saline. When access to the sucrose solution was discontinued for 14 days, the rats lost the ability to discriminate between NTX and saline. We also studied the changes of c-Fos IR in selected brain regions in rats treated with saline versus NTX that were drinking water or 25% sucrose. An injection of NTX or saline resulted in a significant drug, diet, and interaction effect in various brain regions associated with feeding behavior, particularly the amygdala, accumbens, and hypothalamic sites. Thus, we found that ingestion of a sucrose solution results in the ability of rats to reliably discriminate naltrexone administration. In addition, sucrose and naltrexone altered c-Fos IR in an interactive fashion in brain regions known to be involved in ingestion behavior. MDPI 2022-02-22 /pmc/articles/PMC8912831/ /pubmed/35267900 http://dx.doi.org/10.3390/nu14050926 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jewett, David C.
Liyanagamage, Donisha S. N. K.
Avond, Mark A. Vanden
Anderson, Molly A. B.
Twaroski, Kyleigh A.
Marek, Morgan A.
James, Kimberly F.
Pal, Tapasya
Klockars, Anica
Olszewski, Pawel K.
Levine, Allen S.
Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats
title Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats
title_full Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats
title_fullStr Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats
title_full_unstemmed Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats
title_short Chronic Intermittent Sucrose Consumption Facilitates the Ability to Discriminate Opioid Receptor Blockade with Naltrexone in Rats
title_sort chronic intermittent sucrose consumption facilitates the ability to discriminate opioid receptor blockade with naltrexone in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912831/
https://www.ncbi.nlm.nih.gov/pubmed/35267900
http://dx.doi.org/10.3390/nu14050926
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