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RNA-Seq is not required to determine stable reference genes for qPCR normalization

Assessment of differential gene expression by qPCR is heavily influenced by the choice of reference genes. Although numerous statistical approaches have been proposed to determine the best reference genes, they can give rise to conflicting results depending on experimental conditions. Hence, recent...

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Detalles Bibliográficos
Autores principales: Sampathkumar, Nirmal Kumar, Sundaram, Venkat Krishnan, Danthi, Prakroothi S., Barakat, Rasha, Solomon, Shiden, Mondal, Mrityunjoy, Carre, Ivo, El Jalkh, Tatiana, Padilla-Ferrer, Aïda, Grenier, Julien, Massaad, Charbel, Mitchell, Jacqueline C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912902/
https://www.ncbi.nlm.nih.gov/pubmed/35226660
http://dx.doi.org/10.1371/journal.pcbi.1009868

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