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The sampling efficiencies of commercial nasopharyngeal swabs

Upper respiratory tract samples are the most commonly used samples for coronavirus disease 2019 (COVID-19) diagnosis. The samples collected from the nasopharynx are preferred for viral nucleic acids detection. Commercial nasopharyngeal swabs (NPSs) are the major factor that influences the sampling q...

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Detalles Bibliográficos
Autores principales: Wang, Ying, Wang, Geng, Zhang, Lulu, Ren, Lili, Wang, Jianwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chinese Medical Association Publishing House. Published by Elsevier BV. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912975/
https://www.ncbi.nlm.nih.gov/pubmed/35291338
http://dx.doi.org/10.1016/j.bsheal.2022.03.007
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author Wang, Ying
Wang, Geng
Zhang, Lulu
Ren, Lili
Wang, Jianwei
author_facet Wang, Ying
Wang, Geng
Zhang, Lulu
Ren, Lili
Wang, Jianwei
author_sort Wang, Ying
collection PubMed
description Upper respiratory tract samples are the most commonly used samples for coronavirus disease 2019 (COVID-19) diagnosis. The samples collected from the nasopharynx are preferred for viral nucleic acids detection. Commercial nasopharyngeal swabs (NPSs) are the major factor that influences the sampling quality. We here evaluated the acceptability and efficiency of NPSs from five manufacturers by examining the concentration of glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) retrieved from the swabs using the RT-PCR method. Significant different concentrations of GAPDH were detected, ranged from 4.36 × 10(8) copies/mL to 6.98 × 10(10) copies/mL among the five swabs (p < 0.05). The designation of the swab head, with or without tip expansion, had limited influence on the collection efficiency. The discrepancy among the NPSs emphasized the improvement of the swab head material.
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spelling pubmed-89129752022-03-11 The sampling efficiencies of commercial nasopharyngeal swabs Wang, Ying Wang, Geng Zhang, Lulu Ren, Lili Wang, Jianwei Biosaf Health Article Upper respiratory tract samples are the most commonly used samples for coronavirus disease 2019 (COVID-19) diagnosis. The samples collected from the nasopharynx are preferred for viral nucleic acids detection. Commercial nasopharyngeal swabs (NPSs) are the major factor that influences the sampling quality. We here evaluated the acceptability and efficiency of NPSs from five manufacturers by examining the concentration of glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH) retrieved from the swabs using the RT-PCR method. Significant different concentrations of GAPDH were detected, ranged from 4.36 × 10(8) copies/mL to 6.98 × 10(10) copies/mL among the five swabs (p < 0.05). The designation of the swab head, with or without tip expansion, had limited influence on the collection efficiency. The discrepancy among the NPSs emphasized the improvement of the swab head material. Chinese Medical Association Publishing House. Published by Elsevier BV. 2022-04 2022-03-11 /pmc/articles/PMC8912975/ /pubmed/35291338 http://dx.doi.org/10.1016/j.bsheal.2022.03.007 Text en © 2022 Chinese Medical Association Publishing House. Published by Elsevier BV. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Wang, Ying
Wang, Geng
Zhang, Lulu
Ren, Lili
Wang, Jianwei
The sampling efficiencies of commercial nasopharyngeal swabs
title The sampling efficiencies of commercial nasopharyngeal swabs
title_full The sampling efficiencies of commercial nasopharyngeal swabs
title_fullStr The sampling efficiencies of commercial nasopharyngeal swabs
title_full_unstemmed The sampling efficiencies of commercial nasopharyngeal swabs
title_short The sampling efficiencies of commercial nasopharyngeal swabs
title_sort sampling efficiencies of commercial nasopharyngeal swabs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8912975/
https://www.ncbi.nlm.nih.gov/pubmed/35291338
http://dx.doi.org/10.1016/j.bsheal.2022.03.007
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