Cargando…
Comparative assessment of the cell-surface antigens and gene expression profiles of the gingival tissue biomarkers in subjects with fixed functional and removable functional orthodontic appliances
BACKGROUND: This study aimed to examine the cellular components of the gingiva during orthodontic treatment with fixed and removable appliances. The cellular and molecular cues of pathologies of the gingival tissue associated with the use of different orthodontic appliances could be studied. MATERIA...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913387/ https://www.ncbi.nlm.nih.gov/pubmed/35280575 http://dx.doi.org/10.1016/j.sjbs.2021.10.047 |
_version_ | 1784667427059007488 |
---|---|
author | Baeshen, Hosam Ali Alsulaimani, Fahad F. Awadh, Wael Ageeli, Maram Ahmed Abullais, Shahabe Saquib Alqahtani, Nabeeh Abdullah Alshahrani, Mohammad Y. Hosmani, Jagadish Patil, Shankargouda |
author_facet | Baeshen, Hosam Ali Alsulaimani, Fahad F. Awadh, Wael Ageeli, Maram Ahmed Abullais, Shahabe Saquib Alqahtani, Nabeeh Abdullah Alshahrani, Mohammad Y. Hosmani, Jagadish Patil, Shankargouda |
author_sort | Baeshen, Hosam Ali |
collection | PubMed |
description | BACKGROUND: This study aimed to examine the cellular components of the gingiva during orthodontic treatment with fixed and removable appliances. The cellular and molecular cues of pathologies of the gingival tissue associated with the use of different orthodontic appliances could be studied. MATERIALS AND METHODS: Tissue samples of gingiva were received from healthy patients undergoing gingivectomy for aesthetic purpose and from patients with fixed and removable functional orthodontic appliances undergoing gingivectomy for gingival overgrowth. The collected samples were stored in a sterile container with phosphate-buffered saline and to carry out further processes it was transported to the laboratory. RESULTS: Cells positive for ECAD and NCAD were found to be increased in fixed appliances where as CD90 and CD105 positive cells showed no significant difference in all the three groups. CD24 and CD146 positive cells were increased significantly in removable and fixed than normal whereas CD133 positive cells were decreased in removable and fixed than normal. CD44 positive cells showed no noticeable change in all three groups. The gene expression levels of KRT5, SOX2, NANOG, and CXCL5 were found to be significantly increased in removable and fixed appliance groups. However, KRT8, CXCL10, and TIMP1 were increased only in fixed appliance group but CXCL10 showed decreased expression in removable appliance group. KRT6A, MYC, and MMP9 were decreased in fixed appliance group whereas MYC and MMP9 were increased in removable appliance group. KRT6A, KRT8, and TIMP1 showed no significant difference in removable appliance group. CONCLUSION: This study demonstrated essential roles of various genes, showing their contribution in regulating cell proliferation and migration in both the removable and fixed functional appliances. |
format | Online Article Text |
id | pubmed-8913387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-89133872022-03-12 Comparative assessment of the cell-surface antigens and gene expression profiles of the gingival tissue biomarkers in subjects with fixed functional and removable functional orthodontic appliances Baeshen, Hosam Ali Alsulaimani, Fahad F. Awadh, Wael Ageeli, Maram Ahmed Abullais, Shahabe Saquib Alqahtani, Nabeeh Abdullah Alshahrani, Mohammad Y. Hosmani, Jagadish Patil, Shankargouda Saudi J Biol Sci Original Article BACKGROUND: This study aimed to examine the cellular components of the gingiva during orthodontic treatment with fixed and removable appliances. The cellular and molecular cues of pathologies of the gingival tissue associated with the use of different orthodontic appliances could be studied. MATERIALS AND METHODS: Tissue samples of gingiva were received from healthy patients undergoing gingivectomy for aesthetic purpose and from patients with fixed and removable functional orthodontic appliances undergoing gingivectomy for gingival overgrowth. The collected samples were stored in a sterile container with phosphate-buffered saline and to carry out further processes it was transported to the laboratory. RESULTS: Cells positive for ECAD and NCAD were found to be increased in fixed appliances where as CD90 and CD105 positive cells showed no significant difference in all the three groups. CD24 and CD146 positive cells were increased significantly in removable and fixed than normal whereas CD133 positive cells were decreased in removable and fixed than normal. CD44 positive cells showed no noticeable change in all three groups. The gene expression levels of KRT5, SOX2, NANOG, and CXCL5 were found to be significantly increased in removable and fixed appliance groups. However, KRT8, CXCL10, and TIMP1 were increased only in fixed appliance group but CXCL10 showed decreased expression in removable appliance group. KRT6A, MYC, and MMP9 were decreased in fixed appliance group whereas MYC and MMP9 were increased in removable appliance group. KRT6A, KRT8, and TIMP1 showed no significant difference in removable appliance group. CONCLUSION: This study demonstrated essential roles of various genes, showing their contribution in regulating cell proliferation and migration in both the removable and fixed functional appliances. Elsevier 2022-03 2021-10-25 /pmc/articles/PMC8913387/ /pubmed/35280575 http://dx.doi.org/10.1016/j.sjbs.2021.10.047 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Baeshen, Hosam Ali Alsulaimani, Fahad F. Awadh, Wael Ageeli, Maram Ahmed Abullais, Shahabe Saquib Alqahtani, Nabeeh Abdullah Alshahrani, Mohammad Y. Hosmani, Jagadish Patil, Shankargouda Comparative assessment of the cell-surface antigens and gene expression profiles of the gingival tissue biomarkers in subjects with fixed functional and removable functional orthodontic appliances |
title | Comparative assessment of the cell-surface antigens and gene expression profiles of the gingival tissue biomarkers in subjects with fixed functional and removable functional orthodontic appliances |
title_full | Comparative assessment of the cell-surface antigens and gene expression profiles of the gingival tissue biomarkers in subjects with fixed functional and removable functional orthodontic appliances |
title_fullStr | Comparative assessment of the cell-surface antigens and gene expression profiles of the gingival tissue biomarkers in subjects with fixed functional and removable functional orthodontic appliances |
title_full_unstemmed | Comparative assessment of the cell-surface antigens and gene expression profiles of the gingival tissue biomarkers in subjects with fixed functional and removable functional orthodontic appliances |
title_short | Comparative assessment of the cell-surface antigens and gene expression profiles of the gingival tissue biomarkers in subjects with fixed functional and removable functional orthodontic appliances |
title_sort | comparative assessment of the cell-surface antigens and gene expression profiles of the gingival tissue biomarkers in subjects with fixed functional and removable functional orthodontic appliances |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913387/ https://www.ncbi.nlm.nih.gov/pubmed/35280575 http://dx.doi.org/10.1016/j.sjbs.2021.10.047 |
work_keys_str_mv | AT baeshenhosamali comparativeassessmentofthecellsurfaceantigensandgeneexpressionprofilesofthegingivaltissuebiomarkersinsubjectswithfixedfunctionalandremovablefunctionalorthodonticappliances AT alsulaimanifahadf comparativeassessmentofthecellsurfaceantigensandgeneexpressionprofilesofthegingivaltissuebiomarkersinsubjectswithfixedfunctionalandremovablefunctionalorthodonticappliances AT awadhwael comparativeassessmentofthecellsurfaceantigensandgeneexpressionprofilesofthegingivaltissuebiomarkersinsubjectswithfixedfunctionalandremovablefunctionalorthodonticappliances AT ageelimaramahmed comparativeassessmentofthecellsurfaceantigensandgeneexpressionprofilesofthegingivaltissuebiomarkersinsubjectswithfixedfunctionalandremovablefunctionalorthodonticappliances AT abullaisshahabesaquib comparativeassessmentofthecellsurfaceantigensandgeneexpressionprofilesofthegingivaltissuebiomarkersinsubjectswithfixedfunctionalandremovablefunctionalorthodonticappliances AT alqahtaninabeehabdullah comparativeassessmentofthecellsurfaceantigensandgeneexpressionprofilesofthegingivaltissuebiomarkersinsubjectswithfixedfunctionalandremovablefunctionalorthodonticappliances AT alshahranimohammady comparativeassessmentofthecellsurfaceantigensandgeneexpressionprofilesofthegingivaltissuebiomarkersinsubjectswithfixedfunctionalandremovablefunctionalorthodonticappliances AT hosmanijagadish comparativeassessmentofthecellsurfaceantigensandgeneexpressionprofilesofthegingivaltissuebiomarkersinsubjectswithfixedfunctionalandremovablefunctionalorthodonticappliances AT patilshankargouda comparativeassessmentofthecellsurfaceantigensandgeneexpressionprofilesofthegingivaltissuebiomarkersinsubjectswithfixedfunctionalandremovablefunctionalorthodonticappliances |