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Tunable and scalable fabrication of block copolymer-based 3D polymorphic artificial cell membrane array
Owing to their excellent durability, tunable physical properties, and biofunctionality, block copolymer-based membranes provide a platform for various biotechnological applications. However, conventional approaches for fabricating block copolymer membranes produce only planar or suspended polymersom...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913694/ https://www.ncbi.nlm.nih.gov/pubmed/35273189 http://dx.doi.org/10.1038/s41467-022-28960-y |
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author | Kang, Dong-Hyun Han, Won Bae Il Ryu, Hyun Kim, Nam Hyuk Kim, Tae Young Choi, Nakwon Kang, Ji Yoon Yu, Yeon Gyu Kim, Tae Song |
author_facet | Kang, Dong-Hyun Han, Won Bae Il Ryu, Hyun Kim, Nam Hyuk Kim, Tae Young Choi, Nakwon Kang, Ji Yoon Yu, Yeon Gyu Kim, Tae Song |
author_sort | Kang, Dong-Hyun |
collection | PubMed |
description | Owing to their excellent durability, tunable physical properties, and biofunctionality, block copolymer-based membranes provide a platform for various biotechnological applications. However, conventional approaches for fabricating block copolymer membranes produce only planar or suspended polymersome structures, which limits their utilization. This study is the first to demonstrate that an electric-field-assisted self-assembly technique can allow controllable and scalable fabrication of 3-dimensional block copolymer artificial cell membranes (3DBCPMs) immobilized on predefined locations. Topographically and chemically structured microwell array templates facilitate uniform patterning of block copolymers and serve as reactors for the effective growth of 3DBCPMs. Modulating the concentration of the block copolymer and the amplitude/frequency of the electric field generates 3DBCPMs with diverse shapes, controlled sizes, and high stability (100% survival over 50 days). In vitro protein–membrane assays and mimicking of human intestinal organs highlight the potential of 3DBCPMs for a variety of biological applications such as artificial cells, cell-mimetic biosensors, and bioreactors. |
format | Online Article Text |
id | pubmed-8913694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-89136942022-04-01 Tunable and scalable fabrication of block copolymer-based 3D polymorphic artificial cell membrane array Kang, Dong-Hyun Han, Won Bae Il Ryu, Hyun Kim, Nam Hyuk Kim, Tae Young Choi, Nakwon Kang, Ji Yoon Yu, Yeon Gyu Kim, Tae Song Nat Commun Article Owing to their excellent durability, tunable physical properties, and biofunctionality, block copolymer-based membranes provide a platform for various biotechnological applications. However, conventional approaches for fabricating block copolymer membranes produce only planar or suspended polymersome structures, which limits their utilization. This study is the first to demonstrate that an electric-field-assisted self-assembly technique can allow controllable and scalable fabrication of 3-dimensional block copolymer artificial cell membranes (3DBCPMs) immobilized on predefined locations. Topographically and chemically structured microwell array templates facilitate uniform patterning of block copolymers and serve as reactors for the effective growth of 3DBCPMs. Modulating the concentration of the block copolymer and the amplitude/frequency of the electric field generates 3DBCPMs with diverse shapes, controlled sizes, and high stability (100% survival over 50 days). In vitro protein–membrane assays and mimicking of human intestinal organs highlight the potential of 3DBCPMs for a variety of biological applications such as artificial cells, cell-mimetic biosensors, and bioreactors. Nature Publishing Group UK 2022-03-10 /pmc/articles/PMC8913694/ /pubmed/35273189 http://dx.doi.org/10.1038/s41467-022-28960-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kang, Dong-Hyun Han, Won Bae Il Ryu, Hyun Kim, Nam Hyuk Kim, Tae Young Choi, Nakwon Kang, Ji Yoon Yu, Yeon Gyu Kim, Tae Song Tunable and scalable fabrication of block copolymer-based 3D polymorphic artificial cell membrane array |
title | Tunable and scalable fabrication of block copolymer-based 3D polymorphic artificial cell membrane array |
title_full | Tunable and scalable fabrication of block copolymer-based 3D polymorphic artificial cell membrane array |
title_fullStr | Tunable and scalable fabrication of block copolymer-based 3D polymorphic artificial cell membrane array |
title_full_unstemmed | Tunable and scalable fabrication of block copolymer-based 3D polymorphic artificial cell membrane array |
title_short | Tunable and scalable fabrication of block copolymer-based 3D polymorphic artificial cell membrane array |
title_sort | tunable and scalable fabrication of block copolymer-based 3d polymorphic artificial cell membrane array |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913694/ https://www.ncbi.nlm.nih.gov/pubmed/35273189 http://dx.doi.org/10.1038/s41467-022-28960-y |
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