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Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re‐establishment ability

Faecal microbiota transplantation is an emerging medical concept for the treatment of gastrointestinal diseases. This concept, however, has disadvantages as low storability of stool and intensive donor screening. A solution to overcome these problems would be the preservation of an in vitro microbio...

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Autores principales: Haindl, Regina, Totzauer, Lisa, Kulozik, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913864/
https://www.ncbi.nlm.nih.gov/pubmed/35124900
http://dx.doi.org/10.1111/1751-7915.14007
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author Haindl, Regina
Totzauer, Lisa
Kulozik, Ulrich
author_facet Haindl, Regina
Totzauer, Lisa
Kulozik, Ulrich
author_sort Haindl, Regina
collection PubMed
description Faecal microbiota transplantation is an emerging medical concept for the treatment of gastrointestinal diseases. This concept, however, has disadvantages as low storability of stool and intensive donor screening. A solution to overcome these problems would be the preservation of an in vitro microbiota through freeze–drying. However, the influence of the entire preservation process, including cultivation and lyophilization, has not been assessed so far. In this study, the influences of the process steps cultivation, drying and re‐cultivation were determined with cell count, production of metabolites, microbial composition and diversity in the system as evaluation criteria. All pH conditions resulted in stable, culturable communities after re‐cultivation. Cell count, richness, diversity and microbial composition were affected by freeze–drying, but these effects were reversible and vanished during re‐cultivation. Hence, the re‐cultivated system did not differ from the system before drying. The metabolism, measured by short‐chain fatty acids as indicators, showed slight changes due to natural dynamics. Consequently, the cultivation prior to drying was identified to have more influence than the drying itself on the preservation process and therefore the biggest potential for optimization. Hence, the highest similarity with the initial stool sample was obtained with pH 6.0 ‐ 6.5 during cultivation.
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spelling pubmed-89138642022-03-17 Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re‐establishment ability Haindl, Regina Totzauer, Lisa Kulozik, Ulrich Microb Biotechnol Research Articles Faecal microbiota transplantation is an emerging medical concept for the treatment of gastrointestinal diseases. This concept, however, has disadvantages as low storability of stool and intensive donor screening. A solution to overcome these problems would be the preservation of an in vitro microbiota through freeze–drying. However, the influence of the entire preservation process, including cultivation and lyophilization, has not been assessed so far. In this study, the influences of the process steps cultivation, drying and re‐cultivation were determined with cell count, production of metabolites, microbial composition and diversity in the system as evaluation criteria. All pH conditions resulted in stable, culturable communities after re‐cultivation. Cell count, richness, diversity and microbial composition were affected by freeze–drying, but these effects were reversible and vanished during re‐cultivation. Hence, the re‐cultivated system did not differ from the system before drying. The metabolism, measured by short‐chain fatty acids as indicators, showed slight changes due to natural dynamics. Consequently, the cultivation prior to drying was identified to have more influence than the drying itself on the preservation process and therefore the biggest potential for optimization. Hence, the highest similarity with the initial stool sample was obtained with pH 6.0 ‐ 6.5 during cultivation. John Wiley and Sons Inc. 2022-02-06 /pmc/articles/PMC8913864/ /pubmed/35124900 http://dx.doi.org/10.1111/1751-7915.14007 Text en © 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Haindl, Regina
Totzauer, Lisa
Kulozik, Ulrich
Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re‐establishment ability
title Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re‐establishment ability
title_full Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re‐establishment ability
title_fullStr Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re‐establishment ability
title_full_unstemmed Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re‐establishment ability
title_short Preservation by lyophilization of a human intestinal microbiota: influence of the cultivation pH on the drying outcome and re‐establishment ability
title_sort preservation by lyophilization of a human intestinal microbiota: influence of the cultivation ph on the drying outcome and re‐establishment ability
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913864/
https://www.ncbi.nlm.nih.gov/pubmed/35124900
http://dx.doi.org/10.1111/1751-7915.14007
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