Cargando…
Dynamics of long‐term continuous culture of Limnospira indica in an air‐lift photobioreactor
MELiSSA (Microecological Life Support System Alternative) is a developing technology for regenerative life support to enable long‐term human missions in Space and has developed a demonstration Pilot Plant. One of the components of the MELiSSA Pilot Plant system is an 83L external loop air‐lift photo...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913870/ https://www.ncbi.nlm.nih.gov/pubmed/34342154 http://dx.doi.org/10.1111/1751-7915.13882 |
_version_ | 1784667553135591424 |
---|---|
author | Garcia‐Gragera, David Peiro, Enrique Arnau, Carolina Cornet, Jean‐François Dussap, Claude‐Gilles Godia, Francesc |
author_facet | Garcia‐Gragera, David Peiro, Enrique Arnau, Carolina Cornet, Jean‐François Dussap, Claude‐Gilles Godia, Francesc |
author_sort | Garcia‐Gragera, David |
collection | PubMed |
description | MELiSSA (Microecological Life Support System Alternative) is a developing technology for regenerative life support to enable long‐term human missions in Space and has developed a demonstration Pilot Plant. One of the components of the MELiSSA Pilot Plant system is an 83L external loop air‐lift photobioreactor (PBR) where Limnospira indica (previously named Arthrospira sp. PC8005) is axenically cultivated in a continuous operation mode for long‐periods. Its mission is to provide O(2) and consume CO(2) while producing edible material. Biological and process characterization of this PBR is performed by analysing the effect of two main variables, dilution rate (D) and PFD (Photon Flux Density) illumination. A maximum oxygen productivity ([Formula: see text]) of 1.35 mmol l(−1) h(−1) is obtained at a D of 0.025 h(−1) and PFD of 930 µmol m(−2) s(−1). Photoinhibition can occur when a 1 g l(−1) cell density culture is exposed to PFD higher than 1700 µmol m(−2) s(−1). This process is reversible if the illumination is returned to dim light (150 µmol m(−2) s(−1)), proving the cell adaptability and capacity to respond at different illumination conditions. Influence of light intensity in cell composition is also described. Specific photon flux density (qPFD) has a direct effect on phycobiliproteins and chlorophyll content causing a decrease of 62.5% and 47.8%, respectively, when qPFD increases from 6.1 to 19.2 µmol g(−1) s(−1). The same trend is observed for proteins and the opposite for carbohydrate content. Morphological and spiral structural features of L. indica are studied by confocal microscopy, and size distribution parameters are quantified. A direct effect between trichome width and CDW/OD ratio is observed. Changes in size distribution are not correlated with environmental factors, further confirms the adaptation capacity of the cells. The systematic analysis performed provides valuable insights to understand the key performance criteria of continuous culture in air‐lift PBRs. |
format | Online Article Text |
id | pubmed-8913870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89138702022-03-17 Dynamics of long‐term continuous culture of Limnospira indica in an air‐lift photobioreactor Garcia‐Gragera, David Peiro, Enrique Arnau, Carolina Cornet, Jean‐François Dussap, Claude‐Gilles Godia, Francesc Microb Biotechnol Research Articles MELiSSA (Microecological Life Support System Alternative) is a developing technology for regenerative life support to enable long‐term human missions in Space and has developed a demonstration Pilot Plant. One of the components of the MELiSSA Pilot Plant system is an 83L external loop air‐lift photobioreactor (PBR) where Limnospira indica (previously named Arthrospira sp. PC8005) is axenically cultivated in a continuous operation mode for long‐periods. Its mission is to provide O(2) and consume CO(2) while producing edible material. Biological and process characterization of this PBR is performed by analysing the effect of two main variables, dilution rate (D) and PFD (Photon Flux Density) illumination. A maximum oxygen productivity ([Formula: see text]) of 1.35 mmol l(−1) h(−1) is obtained at a D of 0.025 h(−1) and PFD of 930 µmol m(−2) s(−1). Photoinhibition can occur when a 1 g l(−1) cell density culture is exposed to PFD higher than 1700 µmol m(−2) s(−1). This process is reversible if the illumination is returned to dim light (150 µmol m(−2) s(−1)), proving the cell adaptability and capacity to respond at different illumination conditions. Influence of light intensity in cell composition is also described. Specific photon flux density (qPFD) has a direct effect on phycobiliproteins and chlorophyll content causing a decrease of 62.5% and 47.8%, respectively, when qPFD increases from 6.1 to 19.2 µmol g(−1) s(−1). The same trend is observed for proteins and the opposite for carbohydrate content. Morphological and spiral structural features of L. indica are studied by confocal microscopy, and size distribution parameters are quantified. A direct effect between trichome width and CDW/OD ratio is observed. Changes in size distribution are not correlated with environmental factors, further confirms the adaptation capacity of the cells. The systematic analysis performed provides valuable insights to understand the key performance criteria of continuous culture in air‐lift PBRs. John Wiley and Sons Inc. 2021-08-02 /pmc/articles/PMC8913870/ /pubmed/34342154 http://dx.doi.org/10.1111/1751-7915.13882 Text en © 2021 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Garcia‐Gragera, David Peiro, Enrique Arnau, Carolina Cornet, Jean‐François Dussap, Claude‐Gilles Godia, Francesc Dynamics of long‐term continuous culture of Limnospira indica in an air‐lift photobioreactor |
title | Dynamics of long‐term continuous culture of Limnospira indica in an air‐lift photobioreactor |
title_full | Dynamics of long‐term continuous culture of Limnospira indica in an air‐lift photobioreactor |
title_fullStr | Dynamics of long‐term continuous culture of Limnospira indica in an air‐lift photobioreactor |
title_full_unstemmed | Dynamics of long‐term continuous culture of Limnospira indica in an air‐lift photobioreactor |
title_short | Dynamics of long‐term continuous culture of Limnospira indica in an air‐lift photobioreactor |
title_sort | dynamics of long‐term continuous culture of limnospira indica in an air‐lift photobioreactor |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8913870/ https://www.ncbi.nlm.nih.gov/pubmed/34342154 http://dx.doi.org/10.1111/1751-7915.13882 |
work_keys_str_mv | AT garciagrageradavid dynamicsoflongtermcontinuouscultureoflimnospiraindicainanairliftphotobioreactor AT peiroenrique dynamicsoflongtermcontinuouscultureoflimnospiraindicainanairliftphotobioreactor AT arnaucarolina dynamicsoflongtermcontinuouscultureoflimnospiraindicainanairliftphotobioreactor AT cornetjeanfrancois dynamicsoflongtermcontinuouscultureoflimnospiraindicainanairliftphotobioreactor AT dussapclaudegilles dynamicsoflongtermcontinuouscultureoflimnospiraindicainanairliftphotobioreactor AT godiafrancesc dynamicsoflongtermcontinuouscultureoflimnospiraindicainanairliftphotobioreactor |