Folic acid perfusion administration reduced abdominal fat deposition in starter Arbor Acres broilers

With intensive selection for meat production in broilers, excessive fat accumulation is also accompanied and causes economic concerns. Folic acid was reported to be involved in lipid metabolism. The present study was conducted to investigate the role of folic acid in reducing abdominal fat deposition. A total of 105 one-day-old healthy Arbor Acres broilers were randomly distributed into 3 treatments, including the control (Con), saline-perfusion group (NS), and folic acid perfusion group (FA). The growth performance, biochemical characteristics in serum, and lipid metabolism in the liver and abdominal fat tissues were evaluated. Results have shown that folic acid significantly reduced abdominal fat percentage (P < 0.05) and had no effects on BW, ADFI, ADG, and FCR (P > 0.05). Serum triglycerides (TG), total cholesterol (TC), and alanine aminotransferase (ALT) levels were lower in FA group but albumin concentration was higher (P < 0.05). Hepatic ACC, SCD, ELOVL6, PI3K, LDLR, HMGCR, and ABCA1 mRNA abundance were all down-regulated in FA group (P < 0.05) when compared with the Con and NS groups, while CPT1 and PPARα were not affected. In addition, MTTP mRNA abundance was higher in the liver of birds subjected to folic acid (P < 0.05). There was no difference about TG deposition in the liver among all groups based on hematoxylin−eosin (HE) and Oil Red O staining. On the other hand, ELOVL6, PPARγ, IGF1, and TGFβ2 expression were notably decreased in the abdominal fat in FA group (P < 0.05). In conclusion, our data demonstrated that folic acid has reduced abdominal fat percentage by decreasing hepatic lipogenesis and suppressing adipocytes proliferation and differentiation. And the inhibiting effect of adipocytes might be mediated by IGF1 and TGFβ2 down-regulation.