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Methods for the Comprehensive in vivo Analysis of Energy Flux, Fluid Homeostasis, Blood Pressure, and Ventilatory Function in Rodents

Cardiovascular disease represents the leading cause of death in the United States, and metabolic diseases such as obesity represent the primary impediment to improving cardiovascular health. Rodent (mouse and rat) models are widely used to model cardiometabolic disease, and as a result, there is inc...

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Autores principales: Reho, John J., Nakagawa, Pablo, Mouradian, Gary C., Grobe, Connie C., Saravia, Fatima L., Burnett, Colin M. L., Kwitek, Anne E., Kirby, John R., Segar, Jeffrey L., Hodges, Matthew R., Sigmund, Curt D., Grobe, Justin L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914175/
https://www.ncbi.nlm.nih.gov/pubmed/35283781
http://dx.doi.org/10.3389/fphys.2022.855054
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author Reho, John J.
Nakagawa, Pablo
Mouradian, Gary C.
Grobe, Connie C.
Saravia, Fatima L.
Burnett, Colin M. L.
Kwitek, Anne E.
Kirby, John R.
Segar, Jeffrey L.
Hodges, Matthew R.
Sigmund, Curt D.
Grobe, Justin L.
author_facet Reho, John J.
Nakagawa, Pablo
Mouradian, Gary C.
Grobe, Connie C.
Saravia, Fatima L.
Burnett, Colin M. L.
Kwitek, Anne E.
Kirby, John R.
Segar, Jeffrey L.
Hodges, Matthew R.
Sigmund, Curt D.
Grobe, Justin L.
author_sort Reho, John J.
collection PubMed
description Cardiovascular disease represents the leading cause of death in the United States, and metabolic diseases such as obesity represent the primary impediment to improving cardiovascular health. Rodent (mouse and rat) models are widely used to model cardiometabolic disease, and as a result, there is increasing interest in the development of accurate and precise methodologies with sufficiently high resolution to dissect mechanisms controlling cardiometabolic physiology in these small organisms. Further, there is great utility in the development of centralized core facilities furnished with high-throughput equipment configurations and staffed with professional content experts to guide investigators and ensure the rigor and reproducibility of experimental endeavors. Here, we outline the array of specialized equipment and approaches that are employed within the Comprehensive Rodent Metabolic Phenotyping Core (CRMPC) and our collaborating laboratories within the Departments of Physiology, Pediatrics, Microbiology & Immunology, and Biomedical Engineering at the Medical College of Wisconsin (MCW), for the detailed mechanistic dissection of cardiometabolic function in mice and rats. We highlight selected methods for the analysis of body composition and fluid compartmentalization, electrolyte accumulation and flux, energy accumulation and flux, physical activity, ingestive behaviors, ventilatory function, blood pressure, heart rate, autonomic function, and assessment and manipulation of the gut microbiota. Further, we include discussion of the advantages and disadvantages of these approaches for their use with rodent models, and considerations for experimental designs using these methods.
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spelling pubmed-89141752022-03-12 Methods for the Comprehensive in vivo Analysis of Energy Flux, Fluid Homeostasis, Blood Pressure, and Ventilatory Function in Rodents Reho, John J. Nakagawa, Pablo Mouradian, Gary C. Grobe, Connie C. Saravia, Fatima L. Burnett, Colin M. L. Kwitek, Anne E. Kirby, John R. Segar, Jeffrey L. Hodges, Matthew R. Sigmund, Curt D. Grobe, Justin L. Front Physiol Physiology Cardiovascular disease represents the leading cause of death in the United States, and metabolic diseases such as obesity represent the primary impediment to improving cardiovascular health. Rodent (mouse and rat) models are widely used to model cardiometabolic disease, and as a result, there is increasing interest in the development of accurate and precise methodologies with sufficiently high resolution to dissect mechanisms controlling cardiometabolic physiology in these small organisms. Further, there is great utility in the development of centralized core facilities furnished with high-throughput equipment configurations and staffed with professional content experts to guide investigators and ensure the rigor and reproducibility of experimental endeavors. Here, we outline the array of specialized equipment and approaches that are employed within the Comprehensive Rodent Metabolic Phenotyping Core (CRMPC) and our collaborating laboratories within the Departments of Physiology, Pediatrics, Microbiology & Immunology, and Biomedical Engineering at the Medical College of Wisconsin (MCW), for the detailed mechanistic dissection of cardiometabolic function in mice and rats. We highlight selected methods for the analysis of body composition and fluid compartmentalization, electrolyte accumulation and flux, energy accumulation and flux, physical activity, ingestive behaviors, ventilatory function, blood pressure, heart rate, autonomic function, and assessment and manipulation of the gut microbiota. Further, we include discussion of the advantages and disadvantages of these approaches for their use with rodent models, and considerations for experimental designs using these methods. Frontiers Media S.A. 2022-02-25 /pmc/articles/PMC8914175/ /pubmed/35283781 http://dx.doi.org/10.3389/fphys.2022.855054 Text en Copyright © 2022 Reho, Nakagawa, Mouradian, Grobe, Saravia, Burnett, Kwitek, Kirby, Segar, Hodges, Sigmund and Grobe. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Reho, John J.
Nakagawa, Pablo
Mouradian, Gary C.
Grobe, Connie C.
Saravia, Fatima L.
Burnett, Colin M. L.
Kwitek, Anne E.
Kirby, John R.
Segar, Jeffrey L.
Hodges, Matthew R.
Sigmund, Curt D.
Grobe, Justin L.
Methods for the Comprehensive in vivo Analysis of Energy Flux, Fluid Homeostasis, Blood Pressure, and Ventilatory Function in Rodents
title Methods for the Comprehensive in vivo Analysis of Energy Flux, Fluid Homeostasis, Blood Pressure, and Ventilatory Function in Rodents
title_full Methods for the Comprehensive in vivo Analysis of Energy Flux, Fluid Homeostasis, Blood Pressure, and Ventilatory Function in Rodents
title_fullStr Methods for the Comprehensive in vivo Analysis of Energy Flux, Fluid Homeostasis, Blood Pressure, and Ventilatory Function in Rodents
title_full_unstemmed Methods for the Comprehensive in vivo Analysis of Energy Flux, Fluid Homeostasis, Blood Pressure, and Ventilatory Function in Rodents
title_short Methods for the Comprehensive in vivo Analysis of Energy Flux, Fluid Homeostasis, Blood Pressure, and Ventilatory Function in Rodents
title_sort methods for the comprehensive in vivo analysis of energy flux, fluid homeostasis, blood pressure, and ventilatory function in rodents
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914175/
https://www.ncbi.nlm.nih.gov/pubmed/35283781
http://dx.doi.org/10.3389/fphys.2022.855054
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