Cargando…

Hackflex: low-cost, high-throughput, Illumina Nextera Flex library construction

We developed a low-cost method for the production of Illumina-compatible sequencing libraries that allows up to 14 times more libraries for high-throughput Illumina sequencing to be generated for the same cost. We call this new method Hackflex. The quality of library preparation was tested by constr...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gaio, Daniela, Anantanawat, Kay, To, Joyce, Liu, Michael, Monahan, Leigh, Darling, Aaron E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Microbiology Society 2022
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914357/ https://www.ncbi.nlm.nih.gov/pubmed/35014949 http://dx.doi.org/10.1099/mgen.0.000744

Ejemplares similares

Improved workflows for high throughput library preparation using the transposome-based nextera system
por: Lamble, Sarah, et al.
Publicado: (2013)

Assessment of insert sizes and adapter content in fastq data from NexteraXT libraries
por: Turner, Frances S.
Publicado: (2014)

NextClip: an analysis and read preparation tool for Nextera Long Mate Pair libraries
por: Leggett, Richard M., et al.
Publicado: (2014)

Evaluation of a high-throughput, cost-effective Illumina library preparation kit
por: Tvedte, Eric S., et al.
Publicado: (2021)

Post-weaning shifts in microbiome composition and metabolism revealed by over 25 000 pig gut metagenome-assembled genomes
por: Gaio, Daniela, et al.
Publicado: (2021)

A High-Throughput Method for Illumina RNA-Seq Library Preparation
por: Kumar, Ravi, et al.
Publicado: (2012)

Phylogenetic diversity analysis of shotgun metagenomic reads describes gut microbiome development and treatment effects in the post-weaned pig
por: Gaio, Daniela, et al.
Publicado: (2022)

Cross-site comparison of ribosomal depletion kits for Illumina RNAseq library construction
por: Herbert, Zachary T., et al.
Publicado: (2018)

An improved method of constructing degradome library suitable for sequencing using Illumina platform
por: Li, Yong-Fang, et al.
Publicado: (2019)

Paired-end sequencing of Fosmid libraries by Illumina
por: Williams, Louise J.S., et al.
Publicado: (2012)

Use of the melting curve assay as a means for high-throughput quantification of Illumina sequencing libraries
por: Shinozuka, Hiroshi, et al.
Publicado: (2016)

NxRepair: error correction in de novo sequence assembly using Nextera mate pairs
por: Murphy, Rebecca R., et al.
Publicado: (2015)

A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand-Specific Multiplex RNA-Seq
por: Wang, Lin, et al.
Publicado: (2011)

A large-scale metagenomic survey dataset of the post-weaning piglet gut lumen
por: Gaio, Daniela, et al.
Publicado: (2021)

Correction: A Low-Cost Library Construction Protocol and Data Analysis Pipeline for Illumina-Based Strand-Specific Multiplex RNA-Seq
por: Wang, Lin, et al.
Publicado: (2011)

Analyzing and minimizing bias in Illumina sequencing libraries
por: Aird, Daniel, et al.
Publicado: (2010)

Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
por: Aird, Daniel, et al.
Publicado: (2011)

Multiplexed Illumina sequencing libraries from picogram quantities of DNA
por: Bowman, Sarah K, et al.
Publicado: (2013)

AlienTrimmer removes adapter oligonucleotides with high sensitivity in short-insert paired-end reads. Commentary on Turner (2014) Assessment of insert sizes and adapter content in FASTQ data from NexteraXT libraries
por: Criscuolo, Alexis, et al.
Publicado: (2014)

Viral Metagenomics: Analysis of Begomoviruses by Illumina High-Throughput Sequencing
por: Idris, Ali, et al.
Publicado: (2014)

Assessing Illumina technology for the high-throughput sequencing of bacteriophage genomes
por: Rihtman, Branko, et al.
Publicado: (2016)

Adobe Flex 3: using Flex with data services : developing Flex applications with BlazeDS
Publicado: (2008)

A novel ultra high-throughput 16S rRNA gene amplicon sequencing library preparation method for the Illumina HiSeq platform
por: de Muinck, Eric J., et al.
Publicado: (2017)

Adobe Flex 3: importing skins into Flex builder
Publicado: (2008)

A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq
por: Burke, Catherine M., et al.
Publicado: (2016)

Fiber flex
por: Nyberg, Kara A.
Publicado: (2005)

Flex & bison /
por: Levine, John R.
Publicado: (2009)

Flex on Java
por: Allmon, B J, et al.
Publicado: (2011)

Flex & Bison
por: Levine, John
Publicado: (2009)

ReSeq simulates realistic Illumina high-throughput sequencing data
por: Schmeing, Stephan, et al.
Publicado: (2021)

Optimizing illumina next-generation sequencing library preparation for extremely at-biased genomes
por: Oyola, Samuel O, et al.
Publicado: (2012)

Illumina Library Preparation for Sequencing the GC-Rich Fraction of Heterogeneous Genomic DNA
por: Tilak, Marie-Ka, et al.
Publicado: (2018)

Evaluation of the MGISEQ-2000 Sequencing Platform for Illumina Target Capture Sequencing Libraries
por: Lang, Jidong, et al.
Publicado: (2021)

The efficiency of Nextera XT tagmentation depends on G and C bases in the binding motif leading to uneven coverage in bacterial species with low and neutral GC-content
por: Segerman, Bo, et al.
Publicado: (2022)

Flex early evaluation: assessing Flex and your project needs
por: O'Reilly
Publicado: (2007)

A cost-effective method for Illumina small RNA-Seq library preparation using T4 RNA ligase 1 adenylated adapters
por: Chen, Yun-Ru, et al.
Publicado: (2012)

Flex 3 component solutions: build amazing interfaces with Flex components
por: Herrington, Jack
Publicado: (2008)

Adobe Flex Builder 3: using Adobe Flex Builder 3
Publicado: (2008)

Adobe Flex 3: creating and extending Adobe Flex 3 components
Publicado: (2008)

Adobe Flex 3: building and deploying Adobe Flex 3 applications
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_buau79kf3k6o8os2j6v5mg169i