An optimized protocol for in vitro and in cellulo structural determination of the multi-tRNA synthetase complex by cross-linking mass spectrometry

Despite recent advances in structural determination of individual proteins, elucidating the 3-dimensional architecture of large, multiprotein complexes remains challenging, partly because of issues related to structural integrity during purification. Here, we describe a protocol to determine the 3-d...

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Detalles Bibliográficos
Autores principales: Khan, Krishnendu, Baleanu-Gogonea, Camelia, Willard, Belinda, Gogonea, Valentin, Fox, Paul L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914369/
https://www.ncbi.nlm.nih.gov/pubmed/35284842
http://dx.doi.org/10.1016/j.xpro.2022.101201

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914369/
https://www.ncbi.nlm.nih.gov/pubmed/35284842
http://dx.doi.org/10.1016/j.xpro.2022.101201

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