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An optimized measles virus glycoprotein-pseudotyped lentiviral vector production system to promote efficient transduction of human primary B cells
Measles virus envelope pseudotyped LV (MV-LV) can achieve high B cell transduction rates (up to 50%), but suffers from low titers. To overcome current limitations, we developed an optimized MV-LV production protocol that achieved consistent B cell transduction efficiency up to 75%. We detail this pr...
Autores principales: | Vamva, Eirini, Ozog, Stosh, Verhoeyen, Els, James, Richard G., Rawlings, David J., Torbett, Bruce E. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914380/ https://www.ncbi.nlm.nih.gov/pubmed/35284833 http://dx.doi.org/10.1016/j.xpro.2022.101228 |
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