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Protocol to measure inter-centrosome distance in adherent cells using epifluorescence microscopy
We present here a protocol to assay the centrosome separation events at late-G2 phase of the cell cycle by immunofluorescence microscopy. We describe the steps required for imaging and measurement of inter-centrosome distance. Here, we use GAS2L1 as an example, but the protocol can be used to test a...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914381/ https://www.ncbi.nlm.nih.gov/pubmed/35284844 http://dx.doi.org/10.1016/j.xpro.2022.101227 |