A protocol for extracting immunolabeled murine cardiomyocytes of high-quality RNA by laser capture microdissection

We developed a highly efficient, ultrashort immunohistochemistry-laser capture microdissection (IHC-LMD) protocol, which allows microdissection of up to 250 single cardiomyocytes. Before LMD, murine hearts are excised, snap-frozen, and cryosectioned. RNA isolated from LMD material is of high RNA qua...

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Detalles Bibliográficos
Autores principales: Shaalan, Abeer K., Ellison-Hughes, Georgina M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914385/
https://www.ncbi.nlm.nih.gov/pubmed/35284837
http://dx.doi.org/10.1016/j.xpro.2022.101231

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914385/
https://www.ncbi.nlm.nih.gov/pubmed/35284837
http://dx.doi.org/10.1016/j.xpro.2022.101231

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