Cargando…

A protocol for extracting immunolabeled murine cardiomyocytes of high-quality RNA by laser capture microdissection

We developed a highly efficient, ultrashort immunohistochemistry-laser capture microdissection (IHC-LMD) protocol, which allows microdissection of up to 250 single cardiomyocytes. Before LMD, murine hearts are excised, snap-frozen, and cryosectioned. RNA isolated from LMD material is of high RNA qua...

Descripción completa

Detalles Bibliográficos
Autores principales:	Shaalan, Abeer K., Ellison-Hughes, Georgina M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8914385/ https://www.ncbi.nlm.nih.gov/pubmed/35284837 http://dx.doi.org/10.1016/j.xpro.2022.101231

Ejemplares similares

An Optimized Protocol of Laser Capture Microdissection for Tissue-Specific RNA Profiling in a Radish Tap Root
por: Choe, Goh, et al.
Publicado: (2020)

Multiplex immunofluorescence-guided laser capture microdissection for spatial transcriptomics of metastatic melanoma tissues
por: Martinek, Jan, et al.
Publicado: (2022)

Laser Capture Microdissection in Dentistry
por: Chokechanachaisakul, Uraiwan, et al.
Publicado: (2010)

Validation of Laser Capture Microdissection Protocol in Endometriosis Studies
por: da Costa, Katiane de Almeida, et al.
Publicado: (2019)

A Systematic Comparison of Protocols for Recovery of High-Quality RNA from Human Islets Extracted by Laser Capture Microdissection
por: Cefalo, Chiara M. A., et al.
Publicado: (2021)

An optimized protocol for isolation of high‐quality RNA through laser capture microdissection of leaf material
por: Hua, Lei, et al.
Publicado: (2019)

Laser Capture Microdissection Protocol for Xylem Tissues of Woody Plants
por: Blokhina, Olga, et al.
Publicado: (2017)

Hormone immunolabeling in resin-embedded Arabidopsis tissues
por: Herrera-Ubaldo, Humberto, et al.
Publicado: (2023)

Shape decomposition algorithms for laser capture microdissection
por: Selbach, Leonie, et al.
Publicado: (2021)

Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue
por: Kolijn, Kimberley, et al.
Publicado: (2016)

Laser capture microdissection in forensic research: a review
por: Vandewoestyne, Mado, et al.
Publicado: (2010)

Laser capture microdissection of gonads from juvenile zebrafish
por: Jørgensen, Anne, et al.
Publicado: (2009)

The invasive proteome of glioblastoma revealed by laser-capture microdissection
por: Daubon, Thomas, et al.
Publicado: (2019)

High quality RNA from multiple brain regions simultaneously acquired by laser capture microdissection
por: Wang, Wei-Zhi, et al.
Publicado: (2009)

Laser Microdissection-Mediated Isolation of Butterfly Wing Tissue for Spatial Transcriptomics
por: Banerjee, Tirtha Das, et al.
Publicado: (2022)

The Cerebral Microvasculature in Schizophrenia: A Laser Capture Microdissection Study
por: Harris, Laura W., et al.
Publicado: (2008)

Laser capture microdissection for transcriptomic profiles in human skin biopsies
por: Santoro, Silvia, et al.
Publicado: (2018)

Identification of Inner Mucus-Associated Bacteria by Laser Capture Microdissection
por: Chassaing, Benoit, et al.
Publicado: (2018)

Laser Capture Microdissection: A Gear for Pancreatic Cancer Research
por: Rao, Bhavana Hemantha, et al.
Publicado: (2022)

Preparation of the intact rodent organ of Corti for RNAscope and immunolabeling, confocal microscopy, and quantitative analysis
por: Reijntjes, Daniel O.J., et al.
Publicado: (2021)

Protocol for Single-Nucleus Transcriptomics of Diploid and Tetraploid Cardiomyocytes in Murine Hearts
por: Cui, Miao, et al.
Publicado: (2020)

Proteome analysis of human gastric cardia adenocarcinoma by laser capture microdissection
por: Cheng, Yan, et al.
Publicado: (2007)

DNA Profiling of Spermatozoa by Laser Capture Microdissection and Low Volume-PCR
por: Li, Cai-xia, et al.
Publicado: (2011)

Laser capture microdissection: from its principle to applications in research on neurodegeneration
por: Chung, Sook Hyun, et al.
Publicado: (2015)

A high-efficiency trichome collection system by laser capture microdissection
por: Qin, Wei, et al.
Publicado: (2022)

Laser Capture Microdissection of Pancreatic Acinar Cells to Identify Proteomic Alterations in a Murine Model of Caerulein-Induced Pancreatitis
por: Shapiro, John P, et al.
Publicado: (2017)

Optimization of laser capture microdissection and RNA amplification for gene expression profiling of prostate cancer
por: Kube, Dagmar M, et al.
Publicado: (2007)

An Optimised Protocol Harnessing Laser Capture Microdissection for Transcriptomic Analysis on Matched Primary and Metastatic Colorectal Tumours
por: Ong, Chin-Ann J., et al.
Publicado: (2020)

Freeze-fracture replica immunolabelling reveals caveolin-1 in the human cardiomyocyte plasma membrane
por: Robenek, Horst, et al.
Publicado: (2008)

An improved procedure for isolation of high-quality RNA from nematode-infected Arabidopsis roots through laser capture microdissection
por: Anjam, Muhammad Shahzad, et al.
Publicado: (2016)

PAXgene-fixed paraffin-embedded sample is applicable to laser capture microdissection with well-balanced RNA quality and tissue morphology
por: Yamazaki, Masaki, et al.
Publicado: (2018)

Laser capture microdissection enables cellular and molecular studies of tooth root development
por: Sun, Jian-Xun, et al.
Publicado: (2012)

Characterization of Glomerular Diseases Using Proteomic Analysis of Laser Capture Microdissected Glomeruli
por: Satoskar, AA, et al.
Publicado: (2012)

Proteomics Pipeline for Biomarker Discovery of Laser Capture Microdissected Breast Cancer Tissue
por: Liu, Ning Qing, et al.
Publicado: (2012)

Quantitative Proteomics in Laser Capture Microdissected Sleep Nuclei From Rat Brain
por: Miller, Ronald A., et al.
Publicado: (2014)

Implementation and evaluation of amyloidosis subtyping by laser-capture microdissection and tandem mass spectrometry
por: Mollee, Peter, et al.
Publicado: (2016)

Fluorescence-based cell-specific detection for laser-capture microdissection in human brain
por: Rocco, Brad R., et al.
Publicado: (2017)

Laser-Assisted Microdissection
por: Oberbeck, Denesa
Publicado: (2008)

Use of laser microdissection in the analysis of renal-infiltrating T cells in murine lupus
por: Guo, Zhentao, et al.
Publicado: (2014)

Optimizing Laser Capture Microdissection Protocol for Isolating Zone-Specific Cell Populations from Mandibular Condylar Cartilage
por: Basudan, Aisha M., et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_nkqe652vdrnvelf3mlf452l6bs