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Optimization of cationic polymer-mediated transfection for RNA interference

Transfection efficiency was estimated to optimize the conditions for RNA interference (RNAi), including transfection time, validity, and nucleic acid concentration and type, using the EZ Trans Cell Reagent, a cationic polymer. An shRNA against GFP was designed and transfected into cells using the EZ...

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Detalles Bibliográficos
Autores principales:	Fan, Xiaojie, Yang, Jingnan, Wu, Guangyao, Wang, Meiyi, Cheng, Xiaoxia, Liu, Chang, Liu, Qian, Wen, Yanan, Meng, Shuangshuang, Wang, Zhenxing, Lin, Xuhong, An, Lei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Sociedade Brasileira de Genética 2022
Materias:	Cellular, Molecular and Developmental Genetics
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915406/ https://www.ncbi.nlm.nih.gov/pubmed/35275159 http://dx.doi.org/10.1590/1678-4685-GMB-2021-0237

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