Cargando…
Genetic Variants miR-126, miR-146a, miR-196a2, and miR-499 in Polycystic Ovary Syndrome
Introduction: Alterations in certain microRNAs (miRNAs) and their target genes have reported in polycystic ovary syndrome (PCOS) and other disease of the female reproductive system, and so may be potential biomarkers. We hypothesised alterations in the prevalence of four miRNAs single nucleotide pol...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915673/ https://www.ncbi.nlm.nih.gov/pubmed/35996522 http://dx.doi.org/10.3389/bjbs.2021.10209 |
_version_ | 1784668091170422784 |
---|---|
author | Li, R. Yu, Y. Jaafar, S. O. Baghchi, B. Farsimadan, M. Arabipour, I. Vaziri, H. |
author_facet | Li, R. Yu, Y. Jaafar, S. O. Baghchi, B. Farsimadan, M. Arabipour, I. Vaziri, H. |
author_sort | Li, R. |
collection | PubMed |
description | Introduction: Alterations in certain microRNAs (miRNAs) and their target genes have reported in polycystic ovary syndrome (PCOS) and other disease of the female reproductive system, and so may be potential biomarkers. We hypothesised alterations in the prevalence of four miRNAs single nucleotide polymorphism (SNP) variants miR-126 rs4636297, miR-146a rs2910164, miR-196a2 rs11614913, and miR-499 rs3746444 in women with PCOS in comparison to healthy controls. Methods: SNPs in the four miRNAs were determined in 385 patients and 385 controls by standard RT-PCR techniques. Results: SNPs in miR-126 and miR-246a were significant linked with PCOS under the allelic, dominant, co-dominant, and recessive models (all p ≤ 0.01). The SNP in miR-499 was linked to PCOS in allelic (T, p = 0.002), dominant (p = 0.035) and recessive (p = 0.003) models. The SNPs -196a was significant linked to PCOS only in the recessive model (p = 0.037). Combining these SNPs in miR-499, mi146a, miR-196a and miR-126 respectively into allele haplotypes found highly significant odds ratios (95% CI) of 0.40 (0.29–0.54) (p < 0.001) for the C-G-C-G haplotype, and 0.46 (0.30–0.70) (p = 0.002) for the C-C-C-A haplotype (p = 0.002) for PCOS. Conclusion: Single SNPs and haplotype combinations in certain SNPs in miR-126, miR-146a, miR-196a2 and miR-499 are strongly linked to PCOS, and so may be useful predictors of this condition. |
format | Online Article Text |
id | pubmed-8915673 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89156732022-03-15 Genetic Variants miR-126, miR-146a, miR-196a2, and miR-499 in Polycystic Ovary Syndrome Li, R. Yu, Y. Jaafar, S. O. Baghchi, B. Farsimadan, M. Arabipour, I. Vaziri, H. Br J Biomed Sci Health Archive Introduction: Alterations in certain microRNAs (miRNAs) and their target genes have reported in polycystic ovary syndrome (PCOS) and other disease of the female reproductive system, and so may be potential biomarkers. We hypothesised alterations in the prevalence of four miRNAs single nucleotide polymorphism (SNP) variants miR-126 rs4636297, miR-146a rs2910164, miR-196a2 rs11614913, and miR-499 rs3746444 in women with PCOS in comparison to healthy controls. Methods: SNPs in the four miRNAs were determined in 385 patients and 385 controls by standard RT-PCR techniques. Results: SNPs in miR-126 and miR-246a were significant linked with PCOS under the allelic, dominant, co-dominant, and recessive models (all p ≤ 0.01). The SNP in miR-499 was linked to PCOS in allelic (T, p = 0.002), dominant (p = 0.035) and recessive (p = 0.003) models. The SNPs -196a was significant linked to PCOS only in the recessive model (p = 0.037). Combining these SNPs in miR-499, mi146a, miR-196a and miR-126 respectively into allele haplotypes found highly significant odds ratios (95% CI) of 0.40 (0.29–0.54) (p < 0.001) for the C-G-C-G haplotype, and 0.46 (0.30–0.70) (p = 0.002) for the C-C-C-A haplotype (p = 0.002) for PCOS. Conclusion: Single SNPs and haplotype combinations in certain SNPs in miR-126, miR-146a, miR-196a2 and miR-499 are strongly linked to PCOS, and so may be useful predictors of this condition. Frontiers Media S.A. 2022-01-07 /pmc/articles/PMC8915673/ /pubmed/35996522 http://dx.doi.org/10.3389/bjbs.2021.10209 Text en Copyright © 2022 Li, Yu, Jaafar, Baghchi, Farsimadan, Arabipour and Vaziri. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Health Archive Li, R. Yu, Y. Jaafar, S. O. Baghchi, B. Farsimadan, M. Arabipour, I. Vaziri, H. Genetic Variants miR-126, miR-146a, miR-196a2, and miR-499 in Polycystic Ovary Syndrome |
title | Genetic Variants miR-126, miR-146a, miR-196a2, and miR-499 in Polycystic Ovary Syndrome |
title_full | Genetic Variants miR-126, miR-146a, miR-196a2, and miR-499 in Polycystic Ovary Syndrome |
title_fullStr | Genetic Variants miR-126, miR-146a, miR-196a2, and miR-499 in Polycystic Ovary Syndrome |
title_full_unstemmed | Genetic Variants miR-126, miR-146a, miR-196a2, and miR-499 in Polycystic Ovary Syndrome |
title_short | Genetic Variants miR-126, miR-146a, miR-196a2, and miR-499 in Polycystic Ovary Syndrome |
title_sort | genetic variants mir-126, mir-146a, mir-196a2, and mir-499 in polycystic ovary syndrome |
topic | Health Archive |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915673/ https://www.ncbi.nlm.nih.gov/pubmed/35996522 http://dx.doi.org/10.3389/bjbs.2021.10209 |
work_keys_str_mv | AT lir geneticvariantsmir126mir146amir196a2andmir499inpolycysticovarysyndrome AT yuy geneticvariantsmir126mir146amir196a2andmir499inpolycysticovarysyndrome AT jaafarso geneticvariantsmir126mir146amir196a2andmir499inpolycysticovarysyndrome AT baghchib geneticvariantsmir126mir146amir196a2andmir499inpolycysticovarysyndrome AT farsimadanm geneticvariantsmir126mir146amir196a2andmir499inpolycysticovarysyndrome AT arabipouri geneticvariantsmir126mir146amir196a2andmir499inpolycysticovarysyndrome AT vazirih geneticvariantsmir126mir146amir196a2andmir499inpolycysticovarysyndrome |