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Antigenic and conserved peptides from diverse Helicobacter pylori antigens
Since the revolutionary finding of Helicobacter pylori as a common bacterial infection, that a high research effort for its eradication has been conducted. Epitope based-vaccine presents advantages over protein-based, as they can be designed to contain epitopes from diverse proteins, therefore, more...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Netherlands
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8916697/ https://www.ncbi.nlm.nih.gov/pubmed/35277779 http://dx.doi.org/10.1007/s10529-022-03238-x |
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author | Calado, Cecília R. C. |
author_facet | Calado, Cecília R. C. |
author_sort | Calado, Cecília R. C. |
collection | PubMed |
description | Since the revolutionary finding of Helicobacter pylori as a common bacterial infection, that a high research effort for its eradication has been conducted. Epitope based-vaccine presents advantages over protein-based, as they can be designed to contain epitopes from diverse proteins, therefore, more easily representing the immune-variability of the bacterial population, while minimizing the toxicity associated to some whole proteins. In the present work, an iterative method, to design antigenic and conserved B-epitopes from diverse virulent factors of H. pylori, was established. The method considered the trade-off between epitopes antigenicity and conservation among the bacterial population. For the method validation, five virulent factors from H. pylori were selected. From each virulent factor, two epitopes were predicted, each with twelve residues of aminoacids. The corresponding ten peptides were synthesised and evaluated by enzyme-linked immunosorbent assay using polyclonal antibodies raised against a specific H. pylori strain. All ten peptides were recognised by the antibodies and were consequently antigenic and conserved. This result could strongly contribute to the design of a multivalent epitope-based vaccine, representing the immunogenetic variability within the bacterial population, leading to a sustained and effective immunogenic protection. |
format | Online Article Text |
id | pubmed-8916697 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-89166972022-03-14 Antigenic and conserved peptides from diverse Helicobacter pylori antigens Calado, Cecília R. C. Biotechnol Lett Original Research Paper Since the revolutionary finding of Helicobacter pylori as a common bacterial infection, that a high research effort for its eradication has been conducted. Epitope based-vaccine presents advantages over protein-based, as they can be designed to contain epitopes from diverse proteins, therefore, more easily representing the immune-variability of the bacterial population, while minimizing the toxicity associated to some whole proteins. In the present work, an iterative method, to design antigenic and conserved B-epitopes from diverse virulent factors of H. pylori, was established. The method considered the trade-off between epitopes antigenicity and conservation among the bacterial population. For the method validation, five virulent factors from H. pylori were selected. From each virulent factor, two epitopes were predicted, each with twelve residues of aminoacids. The corresponding ten peptides were synthesised and evaluated by enzyme-linked immunosorbent assay using polyclonal antibodies raised against a specific H. pylori strain. All ten peptides were recognised by the antibodies and were consequently antigenic and conserved. This result could strongly contribute to the design of a multivalent epitope-based vaccine, representing the immunogenetic variability within the bacterial population, leading to a sustained and effective immunogenic protection. Springer Netherlands 2022-03-11 2022 /pmc/articles/PMC8916697/ /pubmed/35277779 http://dx.doi.org/10.1007/s10529-022-03238-x Text en © The Author(s), under exclusive licence to Springer Nature B.V. 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Research Paper Calado, Cecília R. C. Antigenic and conserved peptides from diverse Helicobacter pylori antigens |
title | Antigenic and conserved peptides from diverse Helicobacter pylori antigens |
title_full | Antigenic and conserved peptides from diverse Helicobacter pylori antigens |
title_fullStr | Antigenic and conserved peptides from diverse Helicobacter pylori antigens |
title_full_unstemmed | Antigenic and conserved peptides from diverse Helicobacter pylori antigens |
title_short | Antigenic and conserved peptides from diverse Helicobacter pylori antigens |
title_sort | antigenic and conserved peptides from diverse helicobacter pylori antigens |
topic | Original Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8916697/ https://www.ncbi.nlm.nih.gov/pubmed/35277779 http://dx.doi.org/10.1007/s10529-022-03238-x |
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