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GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate
GATA4/5/6 transcription factors play essential, conserved roles in heart development. To understand how GATA4/5/6 modulates the mesoderm-to-cardiac fate transition, we labeled, isolated, and performed single-cell gene expression analysis on cells that express gata5 at precardiac time points spanning...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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American Association for the Advancement of Science
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8916722/ https://www.ncbi.nlm.nih.gov/pubmed/35275720 http://dx.doi.org/10.1126/sciadv.abg0834 |
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author | Song, Mengyi Yuan, Xuefei Racioppi, Claudia Leslie, Meaghan Stutt, Nathan Aleksandrova, Anastasiia Christiaen, Lionel Wilson, Michael D. Scott, Ian C. |
author_facet | Song, Mengyi Yuan, Xuefei Racioppi, Claudia Leslie, Meaghan Stutt, Nathan Aleksandrova, Anastasiia Christiaen, Lionel Wilson, Michael D. Scott, Ian C. |
author_sort | Song, Mengyi |
collection | PubMed |
description | GATA4/5/6 transcription factors play essential, conserved roles in heart development. To understand how GATA4/5/6 modulates the mesoderm-to-cardiac fate transition, we labeled, isolated, and performed single-cell gene expression analysis on cells that express gata5 at precardiac time points spanning zebrafish gastrulation to somitogenesis. We found that most mesendoderm-derived lineages had dynamic gata5/6 expression. In the absence of Gata5/6, the population structure of mesendoderm-derived cells was substantially altered. In addition to the expected absence of cardiac mesoderm, we confirmed a concomitant expansion of cranial-pharyngeal mesoderm. Moreover, Gata5/6 loss led to extensive changes in chromatin accessibility near cardiac and pharyngeal genes. Functional analyses in zebrafish and the tunicate Ciona, which has a single GATA4/5/6 homolog, revealed that GATA4/5/6 acts upstream of tbx1 to exert essential and cell-autonomous roles in promoting cardiac and inhibiting pharyngeal mesoderm identity. Overall, cardiac and pharyngeal mesoderm fate choices are achieved through an evolutionarily conserved GATA4/5/6 regulatory network. |
format | Online Article Text |
id | pubmed-8916722 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-89167222022-03-21 GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate Song, Mengyi Yuan, Xuefei Racioppi, Claudia Leslie, Meaghan Stutt, Nathan Aleksandrova, Anastasiia Christiaen, Lionel Wilson, Michael D. Scott, Ian C. Sci Adv Biomedicine and Life Sciences GATA4/5/6 transcription factors play essential, conserved roles in heart development. To understand how GATA4/5/6 modulates the mesoderm-to-cardiac fate transition, we labeled, isolated, and performed single-cell gene expression analysis on cells that express gata5 at precardiac time points spanning zebrafish gastrulation to somitogenesis. We found that most mesendoderm-derived lineages had dynamic gata5/6 expression. In the absence of Gata5/6, the population structure of mesendoderm-derived cells was substantially altered. In addition to the expected absence of cardiac mesoderm, we confirmed a concomitant expansion of cranial-pharyngeal mesoderm. Moreover, Gata5/6 loss led to extensive changes in chromatin accessibility near cardiac and pharyngeal genes. Functional analyses in zebrafish and the tunicate Ciona, which has a single GATA4/5/6 homolog, revealed that GATA4/5/6 acts upstream of tbx1 to exert essential and cell-autonomous roles in promoting cardiac and inhibiting pharyngeal mesoderm identity. Overall, cardiac and pharyngeal mesoderm fate choices are achieved through an evolutionarily conserved GATA4/5/6 regulatory network. American Association for the Advancement of Science 2022-03-11 /pmc/articles/PMC8916722/ /pubmed/35275720 http://dx.doi.org/10.1126/sciadv.abg0834 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Song, Mengyi Yuan, Xuefei Racioppi, Claudia Leslie, Meaghan Stutt, Nathan Aleksandrova, Anastasiia Christiaen, Lionel Wilson, Michael D. Scott, Ian C. GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate |
title | GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate |
title_full | GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate |
title_fullStr | GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate |
title_full_unstemmed | GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate |
title_short | GATA4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate |
title_sort | gata4/5/6 family transcription factors are conserved determinants of cardiac versus pharyngeal mesoderm fate |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8916722/ https://www.ncbi.nlm.nih.gov/pubmed/35275720 http://dx.doi.org/10.1126/sciadv.abg0834 |
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