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Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish

During cardiac development, endocardial cells (EdCs) produce growth factors to promote myocardial morphogenesis and growth. In particular, EdCs produce neuregulin which is required for ventricular cardiomyocytes (CMs) to seed the multicellular ridges known as trabeculae. Defects in neuregulin signal...

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Autores principales: Qi, Jialing, Rittershaus, Annegret, Priya, Rashmi, Mansingh, Shivani, Stainier, Didier YR, Helker, Christian SM
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8916774/
https://www.ncbi.nlm.nih.gov/pubmed/35225788
http://dx.doi.org/10.7554/eLife.73231
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author Qi, Jialing
Rittershaus, Annegret
Priya, Rashmi
Mansingh, Shivani
Stainier, Didier YR
Helker, Christian SM
author_facet Qi, Jialing
Rittershaus, Annegret
Priya, Rashmi
Mansingh, Shivani
Stainier, Didier YR
Helker, Christian SM
author_sort Qi, Jialing
collection PubMed
description During cardiac development, endocardial cells (EdCs) produce growth factors to promote myocardial morphogenesis and growth. In particular, EdCs produce neuregulin which is required for ventricular cardiomyocytes (CMs) to seed the multicellular ridges known as trabeculae. Defects in neuregulin signaling, or in endocardial sprouting toward CMs, cause hypotrabeculation. However, the mechanisms underlying endocardial sprouting remain largely unknown. Here, we first show by live imaging in zebrafish embryos that EdCs interact with CMs via dynamic membrane protrusions. After touching CMs, these protrusions remain in close contact with their target despite the vigorous cardiac contractions. Loss of the CM-derived peptide Apelin, or of the Apelin receptor, which is expressed in EdCs, leads to reduced endocardial sprouting and hypotrabeculation. Mechanistically, neuregulin signaling requires endocardial protrusions to induce extracellular signal-regulated kinase (Erk) activity in CMs and trigger their delamination. Altogether, these data show that Apelin signaling-dependent endocardial protrusions modulate CM behavior during trabeculation.
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spelling pubmed-89167742022-03-12 Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish Qi, Jialing Rittershaus, Annegret Priya, Rashmi Mansingh, Shivani Stainier, Didier YR Helker, Christian SM eLife Cell Biology During cardiac development, endocardial cells (EdCs) produce growth factors to promote myocardial morphogenesis and growth. In particular, EdCs produce neuregulin which is required for ventricular cardiomyocytes (CMs) to seed the multicellular ridges known as trabeculae. Defects in neuregulin signaling, or in endocardial sprouting toward CMs, cause hypotrabeculation. However, the mechanisms underlying endocardial sprouting remain largely unknown. Here, we first show by live imaging in zebrafish embryos that EdCs interact with CMs via dynamic membrane protrusions. After touching CMs, these protrusions remain in close contact with their target despite the vigorous cardiac contractions. Loss of the CM-derived peptide Apelin, or of the Apelin receptor, which is expressed in EdCs, leads to reduced endocardial sprouting and hypotrabeculation. Mechanistically, neuregulin signaling requires endocardial protrusions to induce extracellular signal-regulated kinase (Erk) activity in CMs and trigger their delamination. Altogether, these data show that Apelin signaling-dependent endocardial protrusions modulate CM behavior during trabeculation. eLife Sciences Publications, Ltd 2022-02-28 /pmc/articles/PMC8916774/ /pubmed/35225788 http://dx.doi.org/10.7554/eLife.73231 Text en © 2022, Qi et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Qi, Jialing
Rittershaus, Annegret
Priya, Rashmi
Mansingh, Shivani
Stainier, Didier YR
Helker, Christian SM
Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish
title Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish
title_full Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish
title_fullStr Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish
title_full_unstemmed Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish
title_short Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish
title_sort apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8916774/
https://www.ncbi.nlm.nih.gov/pubmed/35225788
http://dx.doi.org/10.7554/eLife.73231
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