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Degradation and de novo formation of nine major glucose degradation products during storage of peritoneal dialysis fluids

Reactive glucose degradation products (GDPs) are formed during heat sterilization of glucose-containing peritoneal dialysis fluids (PDFs) and may induce adverse clinical effects. Long periods of storage and/or transport of PDFs before use may lead to de novo formation or degradation of GDPs. Therefo...

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Autores principales: Gensberger-Reigl, Sabrina, Weigel, Ingrid, Stützer, Joachim, Auditore, Andrea, Nikolaus, Tim, Pischetsrieder, Monika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8917136/
https://www.ncbi.nlm.nih.gov/pubmed/35277529
http://dx.doi.org/10.1038/s41598-022-08123-1
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author Gensberger-Reigl, Sabrina
Weigel, Ingrid
Stützer, Joachim
Auditore, Andrea
Nikolaus, Tim
Pischetsrieder, Monika
author_facet Gensberger-Reigl, Sabrina
Weigel, Ingrid
Stützer, Joachim
Auditore, Andrea
Nikolaus, Tim
Pischetsrieder, Monika
author_sort Gensberger-Reigl, Sabrina
collection PubMed
description Reactive glucose degradation products (GDPs) are formed during heat sterilization of glucose-containing peritoneal dialysis fluids (PDFs) and may induce adverse clinical effects. Long periods of storage and/or transport of PDFs before use may lead to de novo formation or degradation of GDPs. Therefore, the present study quantified the GDP profiles of single- and double-chamber PDFs during storage. Glucosone, 3-deoxyglucosone (3-DG), 3-deoxygalactosone (3-DGal), 3,4-dideoxyglucosone-3-ene (3,4-DGE), glyoxal, methylglyoxal (MGO), acetaldehyde, formaldehyde, and 5-hydroxymethylfurfural (5-HMF) were quantified by two validated UHPLC-DAD methods after derivatization with o-phenylenediamine (dicarbonyls) or 2,4-dinitrophenylhydrazine (monocarbonyls). The PDFs were stored at 50 °C for 0, 1, 2, 4, 13, and 26 weeks. The total GDP concentration of single-chamber PDFs did not change considerably during storage (496.6 ± 16.0 µM, 0 weeks; 519.1 ± 13.1 µM, 26 weeks), but individual GDPs were affected differently. 3-DG (− 82.6 µM) and 3-DGal (− 71.3 µM) were degraded, whereas 5-HMF (+ 161.7 µM), glyoxal (+ 32.2 µM), and formaldehyde (+ 12.4 µM) accumulated between 0 and 26 weeks. Acetaldehyde, glucosone, MGO, and 3,4-DGE showed time-dependent formation and degradation. The GDP concentrations in double-chamber fluids were generally lower and differently affected by storage. In conclusion, the changes of GDP concentrations during storage should be considered for the evaluation of clinical effects of PDFs.
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spelling pubmed-89171362022-03-14 Degradation and de novo formation of nine major glucose degradation products during storage of peritoneal dialysis fluids Gensberger-Reigl, Sabrina Weigel, Ingrid Stützer, Joachim Auditore, Andrea Nikolaus, Tim Pischetsrieder, Monika Sci Rep Article Reactive glucose degradation products (GDPs) are formed during heat sterilization of glucose-containing peritoneal dialysis fluids (PDFs) and may induce adverse clinical effects. Long periods of storage and/or transport of PDFs before use may lead to de novo formation or degradation of GDPs. Therefore, the present study quantified the GDP profiles of single- and double-chamber PDFs during storage. Glucosone, 3-deoxyglucosone (3-DG), 3-deoxygalactosone (3-DGal), 3,4-dideoxyglucosone-3-ene (3,4-DGE), glyoxal, methylglyoxal (MGO), acetaldehyde, formaldehyde, and 5-hydroxymethylfurfural (5-HMF) were quantified by two validated UHPLC-DAD methods after derivatization with o-phenylenediamine (dicarbonyls) or 2,4-dinitrophenylhydrazine (monocarbonyls). The PDFs were stored at 50 °C for 0, 1, 2, 4, 13, and 26 weeks. The total GDP concentration of single-chamber PDFs did not change considerably during storage (496.6 ± 16.0 µM, 0 weeks; 519.1 ± 13.1 µM, 26 weeks), but individual GDPs were affected differently. 3-DG (− 82.6 µM) and 3-DGal (− 71.3 µM) were degraded, whereas 5-HMF (+ 161.7 µM), glyoxal (+ 32.2 µM), and formaldehyde (+ 12.4 µM) accumulated between 0 and 26 weeks. Acetaldehyde, glucosone, MGO, and 3,4-DGE showed time-dependent formation and degradation. The GDP concentrations in double-chamber fluids were generally lower and differently affected by storage. In conclusion, the changes of GDP concentrations during storage should be considered for the evaluation of clinical effects of PDFs. Nature Publishing Group UK 2022-03-11 /pmc/articles/PMC8917136/ /pubmed/35277529 http://dx.doi.org/10.1038/s41598-022-08123-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Gensberger-Reigl, Sabrina
Weigel, Ingrid
Stützer, Joachim
Auditore, Andrea
Nikolaus, Tim
Pischetsrieder, Monika
Degradation and de novo formation of nine major glucose degradation products during storage of peritoneal dialysis fluids
title Degradation and de novo formation of nine major glucose degradation products during storage of peritoneal dialysis fluids
title_full Degradation and de novo formation of nine major glucose degradation products during storage of peritoneal dialysis fluids
title_fullStr Degradation and de novo formation of nine major glucose degradation products during storage of peritoneal dialysis fluids
title_full_unstemmed Degradation and de novo formation of nine major glucose degradation products during storage of peritoneal dialysis fluids
title_short Degradation and de novo formation of nine major glucose degradation products during storage of peritoneal dialysis fluids
title_sort degradation and de novo formation of nine major glucose degradation products during storage of peritoneal dialysis fluids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8917136/
https://www.ncbi.nlm.nih.gov/pubmed/35277529
http://dx.doi.org/10.1038/s41598-022-08123-1
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