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Deep tissue localization and sensing using optical microcavity probes

Optical microcavities and microlasers were recently introduced as probes inside living cells and tissues. Their main advantages are spectrally narrow emission lines and high sensitivity to the environment. Despite numerous novel methods for optical imaging in strongly scattering biological tissues,...

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Autores principales: Kavčič, Aljaž, Garvas, Maja, Marinčič, Matevž, Unger, Katrin, Coclite, Anna Maria, Majaron, Boris, Humar, Matjaž
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8917156/
https://www.ncbi.nlm.nih.gov/pubmed/35277496
http://dx.doi.org/10.1038/s41467-022-28904-6
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author Kavčič, Aljaž
Garvas, Maja
Marinčič, Matevž
Unger, Katrin
Coclite, Anna Maria
Majaron, Boris
Humar, Matjaž
author_facet Kavčič, Aljaž
Garvas, Maja
Marinčič, Matevž
Unger, Katrin
Coclite, Anna Maria
Majaron, Boris
Humar, Matjaž
author_sort Kavčič, Aljaž
collection PubMed
description Optical microcavities and microlasers were recently introduced as probes inside living cells and tissues. Their main advantages are spectrally narrow emission lines and high sensitivity to the environment. Despite numerous novel methods for optical imaging in strongly scattering biological tissues, imaging at single-cell resolution beyond the ballistic light transport regime remains very challenging. Here, we show that optical microcavity probes embedded inside cells enable three-dimensional localization and tracking of individual cells over extended time periods, as well as sensing of their environment, at depths well beyond the light transport length. This is achieved by utilizing unique spectral features of the whispering-gallery modes, which are unaffected by tissue scattering, absorption, and autofluorescence. In addition, microcavities can be functionalized for simultaneous sensing of various parameters, such as temperature or pH value, which extends their versatility beyond the capabilities of standard fluorescent labels.
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spelling pubmed-89171562022-04-01 Deep tissue localization and sensing using optical microcavity probes Kavčič, Aljaž Garvas, Maja Marinčič, Matevž Unger, Katrin Coclite, Anna Maria Majaron, Boris Humar, Matjaž Nat Commun Article Optical microcavities and microlasers were recently introduced as probes inside living cells and tissues. Their main advantages are spectrally narrow emission lines and high sensitivity to the environment. Despite numerous novel methods for optical imaging in strongly scattering biological tissues, imaging at single-cell resolution beyond the ballistic light transport regime remains very challenging. Here, we show that optical microcavity probes embedded inside cells enable three-dimensional localization and tracking of individual cells over extended time periods, as well as sensing of their environment, at depths well beyond the light transport length. This is achieved by utilizing unique spectral features of the whispering-gallery modes, which are unaffected by tissue scattering, absorption, and autofluorescence. In addition, microcavities can be functionalized for simultaneous sensing of various parameters, such as temperature or pH value, which extends their versatility beyond the capabilities of standard fluorescent labels. Nature Publishing Group UK 2022-03-11 /pmc/articles/PMC8917156/ /pubmed/35277496 http://dx.doi.org/10.1038/s41467-022-28904-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kavčič, Aljaž
Garvas, Maja
Marinčič, Matevž
Unger, Katrin
Coclite, Anna Maria
Majaron, Boris
Humar, Matjaž
Deep tissue localization and sensing using optical microcavity probes
title Deep tissue localization and sensing using optical microcavity probes
title_full Deep tissue localization and sensing using optical microcavity probes
title_fullStr Deep tissue localization and sensing using optical microcavity probes
title_full_unstemmed Deep tissue localization and sensing using optical microcavity probes
title_short Deep tissue localization and sensing using optical microcavity probes
title_sort deep tissue localization and sensing using optical microcavity probes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8917156/
https://www.ncbi.nlm.nih.gov/pubmed/35277496
http://dx.doi.org/10.1038/s41467-022-28904-6
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