Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat–Thinopyrum intermedium and wheat–Thinopyrum ponticum substitution lines

BACKGROUND: Owing to their excellent resistance to abiotic and biotic stress, Thinopyrum intermedium (2n = 6x = 42, JJJ(s)J(s)StSt) and Th. ponticum (2n = 10x = 70) are both widely utilized in wheat germplasm innovation programs. Disomic substitution lines (DSLs) carrying one pair of alien chromosom...

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Autores principales: Wang, Siwen, Wang, Changyou, Feng, Xianbo, Zhao, Jixin, Deng, Pingchuan, Wang, Yajuan, Zhang, Hong, Liu, Xinlun, Li, Tingdong, Chen, Chunhuan, Wang, Baotong, Ji, Wanquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8917741/
https://www.ncbi.nlm.nih.gov/pubmed/35279089
http://dx.doi.org/10.1186/s12870-022-03496-x
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author Wang, Siwen
Wang, Changyou
Feng, Xianbo
Zhao, Jixin
Deng, Pingchuan
Wang, Yajuan
Zhang, Hong
Liu, Xinlun
Li, Tingdong
Chen, Chunhuan
Wang, Baotong
Ji, Wanquan
author_facet Wang, Siwen
Wang, Changyou
Feng, Xianbo
Zhao, Jixin
Deng, Pingchuan
Wang, Yajuan
Zhang, Hong
Liu, Xinlun
Li, Tingdong
Chen, Chunhuan
Wang, Baotong
Ji, Wanquan
author_sort Wang, Siwen
collection PubMed
description BACKGROUND: Owing to their excellent resistance to abiotic and biotic stress, Thinopyrum intermedium (2n = 6x = 42, JJJ(s)J(s)StSt) and Th. ponticum (2n = 10x = 70) are both widely utilized in wheat germplasm innovation programs. Disomic substitution lines (DSLs) carrying one pair of alien chromosomes are valuable bridge materials for transmission of novel genes, fluorescence in situ hybridization (FISH) karyotype construction and specific molecular marker development. RESULTS: Six wheat–Thinopyrum DSLs derived from crosses between Abbondanza nullisomic lines (2n = 40) and two octoploid Trititrigia lines (2n = 8x = 56), were characterized by sequential FISH–genome in situ hybridization (GISH), multicolor GISH (mc-GISH), and an analysis of the wheat 15 K SNP array combined with molecular marker selection. ES-9 (DS2St (2A)) and ES-10 (DS3St (3D)) are wheat–Th. ponticum DSLs, while ES-23 (DS2St (2A)), ES-24 (DS3St (3D)), ES-25(DS2St (2B)), and ES-26 (DS2St (2D)) are wheat–Th. intermedium DSLs. ES-9, ES-23, ES-25 and ES-26 conferred high thousand-kernel weight and stripe rust resistance at adult stages, while ES-10 and ES-24 were highly resistant to stripe rust at all stages. Furthermore, cytological analysis showed that the alien chromosomes belonging to the same homoeologous group (2 or 3) derived from different donors carried the same FISH karyotype and could form a bivalent. Based on specific-locus amplified fragment sequencing (SLAF-seq), two 2St-chromosome-specific markers (PTH-005 and PTH-013) and two 3St-chromosome-specific markers (PTH-113 and PTH-135) were developed. CONCLUSIONS: The six wheat–Thinopyrum DSLs conferring stripe rust resistance can be used as bridging parents for transmission of valuable resistance genes. The utility of PTH-113 and PTH-135 in a BC1F2 population showed that the newly developed markers could be useful tools for efficient identification of St chromosomes in a common wheat background. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03496-x.
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spelling pubmed-89177412022-03-21 Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat–Thinopyrum intermedium and wheat–Thinopyrum ponticum substitution lines Wang, Siwen Wang, Changyou Feng, Xianbo Zhao, Jixin Deng, Pingchuan Wang, Yajuan Zhang, Hong Liu, Xinlun Li, Tingdong Chen, Chunhuan Wang, Baotong Ji, Wanquan BMC Plant Biol Research BACKGROUND: Owing to their excellent resistance to abiotic and biotic stress, Thinopyrum intermedium (2n = 6x = 42, JJJ(s)J(s)StSt) and Th. ponticum (2n = 10x = 70) are both widely utilized in wheat germplasm innovation programs. Disomic substitution lines (DSLs) carrying one pair of alien chromosomes are valuable bridge materials for transmission of novel genes, fluorescence in situ hybridization (FISH) karyotype construction and specific molecular marker development. RESULTS: Six wheat–Thinopyrum DSLs derived from crosses between Abbondanza nullisomic lines (2n = 40) and two octoploid Trititrigia lines (2n = 8x = 56), were characterized by sequential FISH–genome in situ hybridization (GISH), multicolor GISH (mc-GISH), and an analysis of the wheat 15 K SNP array combined with molecular marker selection. ES-9 (DS2St (2A)) and ES-10 (DS3St (3D)) are wheat–Th. ponticum DSLs, while ES-23 (DS2St (2A)), ES-24 (DS3St (3D)), ES-25(DS2St (2B)), and ES-26 (DS2St (2D)) are wheat–Th. intermedium DSLs. ES-9, ES-23, ES-25 and ES-26 conferred high thousand-kernel weight and stripe rust resistance at adult stages, while ES-10 and ES-24 were highly resistant to stripe rust at all stages. Furthermore, cytological analysis showed that the alien chromosomes belonging to the same homoeologous group (2 or 3) derived from different donors carried the same FISH karyotype and could form a bivalent. Based on specific-locus amplified fragment sequencing (SLAF-seq), two 2St-chromosome-specific markers (PTH-005 and PTH-013) and two 3St-chromosome-specific markers (PTH-113 and PTH-135) were developed. CONCLUSIONS: The six wheat–Thinopyrum DSLs conferring stripe rust resistance can be used as bridging parents for transmission of valuable resistance genes. The utility of PTH-113 and PTH-135 in a BC1F2 population showed that the newly developed markers could be useful tools for efficient identification of St chromosomes in a common wheat background. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-022-03496-x. BioMed Central 2022-03-12 /pmc/articles/PMC8917741/ /pubmed/35279089 http://dx.doi.org/10.1186/s12870-022-03496-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wang, Siwen
Wang, Changyou
Feng, Xianbo
Zhao, Jixin
Deng, Pingchuan
Wang, Yajuan
Zhang, Hong
Liu, Xinlun
Li, Tingdong
Chen, Chunhuan
Wang, Baotong
Ji, Wanquan
Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat–Thinopyrum intermedium and wheat–Thinopyrum ponticum substitution lines
title Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat–Thinopyrum intermedium and wheat–Thinopyrum ponticum substitution lines
title_full Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat–Thinopyrum intermedium and wheat–Thinopyrum ponticum substitution lines
title_fullStr Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat–Thinopyrum intermedium and wheat–Thinopyrum ponticum substitution lines
title_full_unstemmed Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat–Thinopyrum intermedium and wheat–Thinopyrum ponticum substitution lines
title_short Molecular cytogenetics and development of St-chromosome-specific molecular markers of novel stripe rust resistant wheat–Thinopyrum intermedium and wheat–Thinopyrum ponticum substitution lines
title_sort molecular cytogenetics and development of st-chromosome-specific molecular markers of novel stripe rust resistant wheat–thinopyrum intermedium and wheat–thinopyrum ponticum substitution lines
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8917741/
https://www.ncbi.nlm.nih.gov/pubmed/35279089
http://dx.doi.org/10.1186/s12870-022-03496-x
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