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Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset
Genetic modification of nonhuman primate (NHP) zygotes is a useful method for the development of NHP models of human diseases. This review summarizes the recent advances in the development of assisted reproductive and genetic manipulation techniques in NHP, providing the basis for the generation of...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918153/ https://www.ncbi.nlm.nih.gov/pubmed/33693670 http://dx.doi.org/10.1093/ilar/ilab002 |
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author | Park, Jung Eun Sasaki, Erika |
author_facet | Park, Jung Eun Sasaki, Erika |
author_sort | Park, Jung Eun |
collection | PubMed |
description | Genetic modification of nonhuman primate (NHP) zygotes is a useful method for the development of NHP models of human diseases. This review summarizes the recent advances in the development of assisted reproductive and genetic manipulation techniques in NHP, providing the basis for the generation of genetically modified NHP disease models. In this study, we review assisted reproductive techniques, including ovarian stimulation, in vitro maturation of oocytes, in vitro fertilization, embryo culture, embryo transfer, and intracytoplasmic sperm injection protocols in marmosets. Furthermore, we review genetic manipulation techniques, including transgenic strategies, target gene knock-out and knock-in using gene editing protocols, and newly developed gene-editing approaches that may potentially impact the production of genetically manipulated NHP models. We further discuss the progress of assisted reproductive and genetic manipulation techniques in NHP; future prospects on genetically modified NHP models for biomedical research are also highlighted. |
format | Online Article Text |
id | pubmed-8918153 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-89181532022-03-14 Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset Park, Jung Eun Sasaki, Erika ILAR J Review Genetic modification of nonhuman primate (NHP) zygotes is a useful method for the development of NHP models of human diseases. This review summarizes the recent advances in the development of assisted reproductive and genetic manipulation techniques in NHP, providing the basis for the generation of genetically modified NHP disease models. In this study, we review assisted reproductive techniques, including ovarian stimulation, in vitro maturation of oocytes, in vitro fertilization, embryo culture, embryo transfer, and intracytoplasmic sperm injection protocols in marmosets. Furthermore, we review genetic manipulation techniques, including transgenic strategies, target gene knock-out and knock-in using gene editing protocols, and newly developed gene-editing approaches that may potentially impact the production of genetically manipulated NHP models. We further discuss the progress of assisted reproductive and genetic manipulation techniques in NHP; future prospects on genetically modified NHP models for biomedical research are also highlighted. Oxford University Press 2021-03-08 /pmc/articles/PMC8918153/ /pubmed/33693670 http://dx.doi.org/10.1093/ilar/ilab002 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the National Academies of Sciences, Engineering, and Medicine. All rights reserved. For permissions, please email: journals.permissions@oup.com https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Review Park, Jung Eun Sasaki, Erika Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset |
title | Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset |
title_full | Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset |
title_fullStr | Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset |
title_full_unstemmed | Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset |
title_short | Assisted Reproductive Techniques and Genetic Manipulation in the Common Marmoset |
title_sort | assisted reproductive techniques and genetic manipulation in the common marmoset |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918153/ https://www.ncbi.nlm.nih.gov/pubmed/33693670 http://dx.doi.org/10.1093/ilar/ilab002 |
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