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Epigenetic Dysregulation of BRDT Gene in Testis Tissues of Infertile Men: Case-Control Study
OBJECTIVE: Bromodomain testis associated (BRDT), a testis-specific member of the Bromo- and Extra-Terrminal domain (BET) protein family, is involved in spermatogenesis and, more specifically, chromatin remodeling. In the post-meiotic spermatogenic cells, BRDT protein binds to the hyperacetylated his...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918272/ https://www.ncbi.nlm.nih.gov/pubmed/35279966 http://dx.doi.org/10.22074/cellj.2022.7724 |
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author | Kohandani, Fereshteh Jazireian, Parham Favaedi, Raha Sadighi Gilani, Mohammad Ali Moshtaghioun, Seyed Mohammad Shahhoseini, Maryam |
author_facet | Kohandani, Fereshteh Jazireian, Parham Favaedi, Raha Sadighi Gilani, Mohammad Ali Moshtaghioun, Seyed Mohammad Shahhoseini, Maryam |
author_sort | Kohandani, Fereshteh |
collection | PubMed |
description | OBJECTIVE: Bromodomain testis associated (BRDT), a testis-specific member of the Bromo- and Extra-Terrminal domain (BET) protein family, is involved in spermatogenesis and, more specifically, chromatin remodeling. In the post-meiotic spermatogenic cells, BRDT protein binds to the hyperacetylated histones and facilitates their replacement with transition proteins (TPs), particularly protamines, which are essential for chromatin condensation. The current research was conducted to assess the expression and epigenetic profile of BRDT in the testis tissues of infertile men. MATERIALS AND METHODS: In this case-control study, three groups were included: positive control group: obstructive azoospermia (OA, n=10), round spermatid maturation arrest group (SMA, n=10) and negative control group: sertoli cell- only syndrome (SCOS, n=10). Using quantitative real-time polymerase chain reaction (PCR), the expression profile of BRDT was generated. Also, ChIP-real time PCR was used to measure the following histone marks: H3K9ac, H3K9me3, H3K4me3, H3K27me3 on the promoter region of BRDT. RESULTS: Our data indicated that BRDT expression decreased in the SMA group in comparison with the positive control group and this finding is in line with the ChIP results obtained in this group. CONCLUSION: Based on these data, we postulate that BRDT gene has a vital role in the spermatogenesis and its decreased expression due to an aberrant epigenetic signaling might be associated with male infertility. |
format | Online Article Text |
id | pubmed-8918272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-89182722022-04-22 Epigenetic Dysregulation of BRDT Gene in Testis Tissues of Infertile Men: Case-Control Study Kohandani, Fereshteh Jazireian, Parham Favaedi, Raha Sadighi Gilani, Mohammad Ali Moshtaghioun, Seyed Mohammad Shahhoseini, Maryam Cell J Original Article OBJECTIVE: Bromodomain testis associated (BRDT), a testis-specific member of the Bromo- and Extra-Terrminal domain (BET) protein family, is involved in spermatogenesis and, more specifically, chromatin remodeling. In the post-meiotic spermatogenic cells, BRDT protein binds to the hyperacetylated histones and facilitates their replacement with transition proteins (TPs), particularly protamines, which are essential for chromatin condensation. The current research was conducted to assess the expression and epigenetic profile of BRDT in the testis tissues of infertile men. MATERIALS AND METHODS: In this case-control study, three groups were included: positive control group: obstructive azoospermia (OA, n=10), round spermatid maturation arrest group (SMA, n=10) and negative control group: sertoli cell- only syndrome (SCOS, n=10). Using quantitative real-time polymerase chain reaction (PCR), the expression profile of BRDT was generated. Also, ChIP-real time PCR was used to measure the following histone marks: H3K9ac, H3K9me3, H3K4me3, H3K27me3 on the promoter region of BRDT. RESULTS: Our data indicated that BRDT expression decreased in the SMA group in comparison with the positive control group and this finding is in line with the ChIP results obtained in this group. CONCLUSION: Based on these data, we postulate that BRDT gene has a vital role in the spermatogenesis and its decreased expression due to an aberrant epigenetic signaling might be associated with male infertility. Royan Institute 2022-02 2022-02-21 /pmc/articles/PMC8918272/ /pubmed/35279966 http://dx.doi.org/10.22074/cellj.2022.7724 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. https://creativecommons.org/licenses/by-nc/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial 3.0 (CC BY-NC 3.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kohandani, Fereshteh Jazireian, Parham Favaedi, Raha Sadighi Gilani, Mohammad Ali Moshtaghioun, Seyed Mohammad Shahhoseini, Maryam Epigenetic Dysregulation of BRDT Gene in Testis Tissues of Infertile Men: Case-Control Study |
title | Epigenetic Dysregulation of BRDT Gene in Testis Tissues of
Infertile Men: Case-Control Study |
title_full | Epigenetic Dysregulation of BRDT Gene in Testis Tissues of
Infertile Men: Case-Control Study |
title_fullStr | Epigenetic Dysregulation of BRDT Gene in Testis Tissues of
Infertile Men: Case-Control Study |
title_full_unstemmed | Epigenetic Dysregulation of BRDT Gene in Testis Tissues of
Infertile Men: Case-Control Study |
title_short | Epigenetic Dysregulation of BRDT Gene in Testis Tissues of
Infertile Men: Case-Control Study |
title_sort | epigenetic dysregulation of brdt gene in testis tissues of
infertile men: case-control study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8918272/ https://www.ncbi.nlm.nih.gov/pubmed/35279966 http://dx.doi.org/10.22074/cellj.2022.7724 |
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